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Title: X-ray diffraction reveals blunt-force loading threshold for nanoscopic structural change in ex vivo neuronal tissues

Abstract

An ex vivo blunt-force loading experiment is disclosed that may, in the future, provide insight into the molecular structural changes occurring in load-induced conditions such as traumatic brain injury (TBI). TBI appears to manifest in changes in multiple structures and elements within the brain and nervous system. Individuals with a TBI may suffer from cognitive and/or behavioral impairments which can adversely affect their quality of life. Information on the injury threshold of tissue loading for mammalian neurons is critical in the development of a quantified neuronal-level dose-response model. Such a model could aid in the discovery of enhanced methods for TBI detection, treatment and prevention. At the present time, thresholds of mechanical load leading to direct force-coupled nanostructural changes in neurons are unknown. In this study, we make use of the fact that changes in the structure and periodicity of myelin may indicate neurological damage and can be detected with X-ray diffraction (XRD). XRD allows access to a nanoscopic resolution range not readily achieved by alternative methods, nor does the experimental methodology require chemical sample fixation. In this study, XRD was used to evaluate the affects of controlled mechanical loading on myelin packing structure in ex vivo optic nervemore » samples. By using a series of crush tests on isolated optic nerves a quantified baseline for mechanical load was found to induce changes in the packing structure of myelin. To the authors' knowledge, this is the first report of its kind.« less

Authors:
 [1]; ORCiD logo [1];  [2];  [1];  [1];  [1];  [3];  [2];  [2]
  1. Illinois Inst. of Technology, Chicago, IL (United States)
  2. Argonne National Lab. (ANL), Lemont, IL (United States)
  3. Army Research Lab., Adelphi, MD (United States)
Publication Date:
Research Org.:
Argonne National Laboratory (ANL), Lemont, IL (United States)
Sponsoring Org.:
National Institutes of Health (NIH); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1504478
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Journal of Synchrotron Radiation (Online)
Additional Journal Information:
Journal Volume: 26; Journal Issue: 1; Journal ID: ISSN 1600-5775
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
73 NUCLEAR PHYSICS AND RADIATION PHYSICS; blunt-force; ex vivo; loading; myelin; x-ray diffraction

Citation Formats

Orgel, Joseph, Madhurapantula, Rama S., Eidsmore, Ashley, Wang, Meng, Dutov, Pavel, Modrich, Charles D., Antipova, Olga, McDonald, Jason, and Satapathy, Sikhanda. X-ray diffraction reveals blunt-force loading threshold for nanoscopic structural change in ex vivo neuronal tissues. United States: N. p., 2018. Web. doi:10.1107/S1600577518015035.
Orgel, Joseph, Madhurapantula, Rama S., Eidsmore, Ashley, Wang, Meng, Dutov, Pavel, Modrich, Charles D., Antipova, Olga, McDonald, Jason, & Satapathy, Sikhanda. X-ray diffraction reveals blunt-force loading threshold for nanoscopic structural change in ex vivo neuronal tissues. United States. doi:10.1107/S1600577518015035.
Orgel, Joseph, Madhurapantula, Rama S., Eidsmore, Ashley, Wang, Meng, Dutov, Pavel, Modrich, Charles D., Antipova, Olga, McDonald, Jason, and Satapathy, Sikhanda. Tue . "X-ray diffraction reveals blunt-force loading threshold for nanoscopic structural change in ex vivo neuronal tissues". United States. doi:10.1107/S1600577518015035.
@article{osti_1504478,
title = {X-ray diffraction reveals blunt-force loading threshold for nanoscopic structural change in ex vivo neuronal tissues},
author = {Orgel, Joseph and Madhurapantula, Rama S. and Eidsmore, Ashley and Wang, Meng and Dutov, Pavel and Modrich, Charles D. and Antipova, Olga and McDonald, Jason and Satapathy, Sikhanda},
abstractNote = {An ex vivo blunt-force loading experiment is disclosed that may, in the future, provide insight into the molecular structural changes occurring in load-induced conditions such as traumatic brain injury (TBI). TBI appears to manifest in changes in multiple structures and elements within the brain and nervous system. Individuals with a TBI may suffer from cognitive and/or behavioral impairments which can adversely affect their quality of life. Information on the injury threshold of tissue loading for mammalian neurons is critical in the development of a quantified neuronal-level dose-response model. Such a model could aid in the discovery of enhanced methods for TBI detection, treatment and prevention. At the present time, thresholds of mechanical load leading to direct force-coupled nanostructural changes in neurons are unknown. In this study, we make use of the fact that changes in the structure and periodicity of myelin may indicate neurological damage and can be detected with X-ray diffraction (XRD). XRD allows access to a nanoscopic resolution range not readily achieved by alternative methods, nor does the experimental methodology require chemical sample fixation. In this study, XRD was used to evaluate the affects of controlled mechanical loading on myelin packing structure in ex vivo optic nerve samples. By using a series of crush tests on isolated optic nerves a quantified baseline for mechanical load was found to induce changes in the packing structure of myelin. To the authors' knowledge, this is the first report of its kind.},
doi = {10.1107/S1600577518015035},
journal = {Journal of Synchrotron Radiation (Online)},
issn = {1600-5775},
number = 1,
volume = 26,
place = {United States},
year = {2018},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on October 23, 2019
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