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Title: Molecular response of poplar to single and combined ozone and drought

Abstract

High concentration in ground-level ozone (O 3) and water deficit affect forest ecosystems service. Earlier we found intercellular CO 2 concentration and isoprene emission were affected by the combination of O 3 and drought, but the molecular mechanisms controlling these phenotypes are still open questions. In this study, we examined the stomatal conductance (g s) and transcriptome changes in an O 3-sensitive hybrid poplar exposed to two O 3 levels [charcoal-filtered ambient air (CF) and non-filtered ambient air plus 40 ppb (NF40)] and two water conditions [well-watered (W) and moderate drought (D)]. NF40 reduced the g s more under D than W. We identified the differentially expressed genes (DEGs) from pairwise comparisons and found the poplar's molecular response to drought was counteracted by elevated O 3. From nine clusters obtained through K-means clustering, 12 core transcription factors were identified. DEGs involved in isoprene biosynthesis and phytohormones signal pathways indicate the molecular response and stomatal closure of poplar under O 3 and/or drought might be through MEP/DOXP and ABA-dependent pathways. In addition, 102 Helitrons capturing DEGs were involved in response to O3 and/or drought and related with ABA-dependent pathway. This integrated analysis provides multi-dimensional insights to understand the molecular response to themore » combination of O 3 and drought.« less

Authors:
ORCiD logo [1];  [2];  [3]; ORCiD logo [3];  [2]
  1. Chinese Academy of Sciences (CAS), Beijing (China); Chinese Academy of Forestry, Beijing (China); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
  2. Chinese Academy of Sciences (CAS), Beijing (China)
  3. Chinese Academy of Forestry, Beijing (China)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1503995
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Science of the Total Environment
Additional Journal Information:
Journal Volume: 655; Journal Issue: C; Journal ID: ISSN 0048-9697
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Climate change; Drought; Ground-level ozone; Poplar; Transcriptome; Helitrons; Protein-protein interaction (PPI) network

Citation Formats

Zhang, Jin, Gao, Feng, Jia, Huixia, Hu, Jianjun, and Feng, Zhaozhong. Molecular response of poplar to single and combined ozone and drought. United States: N. p., 2018. Web. doi:10.1016/j.scitotenv.2018.11.195.
Zhang, Jin, Gao, Feng, Jia, Huixia, Hu, Jianjun, & Feng, Zhaozhong. Molecular response of poplar to single and combined ozone and drought. United States. doi:10.1016/j.scitotenv.2018.11.195.
Zhang, Jin, Gao, Feng, Jia, Huixia, Hu, Jianjun, and Feng, Zhaozhong. Thu . "Molecular response of poplar to single and combined ozone and drought". United States. doi:10.1016/j.scitotenv.2018.11.195.
@article{osti_1503995,
title = {Molecular response of poplar to single and combined ozone and drought},
author = {Zhang, Jin and Gao, Feng and Jia, Huixia and Hu, Jianjun and Feng, Zhaozhong},
abstractNote = {High concentration in ground-level ozone (O3) and water deficit affect forest ecosystems service. Earlier we found intercellular CO2 concentration and isoprene emission were affected by the combination of O3 and drought, but the molecular mechanisms controlling these phenotypes are still open questions. In this study, we examined the stomatal conductance (gs) and transcriptome changes in an O3-sensitive hybrid poplar exposed to two O3 levels [charcoal-filtered ambient air (CF) and non-filtered ambient air plus 40 ppb (NF40)] and two water conditions [well-watered (W) and moderate drought (D)]. NF40 reduced the gs more under D than W. We identified the differentially expressed genes (DEGs) from pairwise comparisons and found the poplar's molecular response to drought was counteracted by elevated O3. From nine clusters obtained through K-means clustering, 12 core transcription factors were identified. DEGs involved in isoprene biosynthesis and phytohormones signal pathways indicate the molecular response and stomatal closure of poplar under O3 and/or drought might be through MEP/DOXP and ABA-dependent pathways. In addition, 102 Helitrons capturing DEGs were involved in response to O3 and/or drought and related with ABA-dependent pathway. This integrated analysis provides multi-dimensional insights to understand the molecular response to the combination of O3 and drought.},
doi = {10.1016/j.scitotenv.2018.11.195},
journal = {Science of the Total Environment},
issn = {0048-9697},
number = C,
volume = 655,
place = {United States},
year = {2018},
month = {11}
}

Journal Article:
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