Lactic acid production by pellet-form Rhizopus oryzae in a submerged system
Abstract
Rhizopus oryzae NRRL 395 produces optically pure L(+)-lactic acid that is highly preferred for the production of environmentally benign polymers. With xylose as the carbon source for cultivation, it can be self-immobilized as pellets with a size of about 1 mm. Repeated usage of the same pellets by transferring them into fresh media every time when the glucose was exhausted over a period of 22 d yielded 1742 or 2001 g/L lactic acid total (based on 100-mL working volume) depending on the media used. Lactic acid is known to be a strong inhibitor for both growth and production, and it can be removed continuously by the adsorption on the PVP resin. With the fermenter-adsorber system, the fermentation can be performed as effectively as the ones with added neutralizing agents, such as calcium carbonate and sodium hydroxide. One problem of the fermenter-adsorber system is that lower production was obtained than in shake flasks; hence, proper reactor design is necessary to improve the process.
- Authors:
-
- Purdue Univ., West Lafayette, IN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 150395
- Report Number(s):
- CONF-940526-
TRN: 95:007973-0007
- Resource Type:
- Conference
- Resource Relation:
- Conference: 16. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 9-13 May 1994; Other Information: PBD: 1995; Related Information: Is Part Of Sixteenth symposium on biotechnology for fuels and chemicals; Davison, B.H. [ed.] [Oak Ridge National Lab., TN (United States)]; Wyman, C.E. [ed.] [National Renewable Energy Lab., Golden, CO (United States)]; PB: 823 p.
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 55 BIOLOGY AND MEDICINE, BASIC STUDIES; LACTIC ACID; BIOSYNTHESIS; RHIZOPUS; IMMOBILIZED CELLS; ADSORPTION; FERMENTATION; GLUCOSE; POLYMERS; XYLOSE; PELLETS; BIOTECHNOLOGY
Citation Formats
Yang, C W, Lu, Z, and Tsao, G T. Lactic acid production by pellet-form Rhizopus oryzae in a submerged system. United States: N. p., 1995.
Web.
Yang, C W, Lu, Z, & Tsao, G T. Lactic acid production by pellet-form Rhizopus oryzae in a submerged system. United States.
Yang, C W, Lu, Z, and Tsao, G T. 1995.
"Lactic acid production by pellet-form Rhizopus oryzae in a submerged system". United States.
@article{osti_150395,
title = {Lactic acid production by pellet-form Rhizopus oryzae in a submerged system},
author = {Yang, C W and Lu, Z and Tsao, G T},
abstractNote = {Rhizopus oryzae NRRL 395 produces optically pure L(+)-lactic acid that is highly preferred for the production of environmentally benign polymers. With xylose as the carbon source for cultivation, it can be self-immobilized as pellets with a size of about 1 mm. Repeated usage of the same pellets by transferring them into fresh media every time when the glucose was exhausted over a period of 22 d yielded 1742 or 2001 g/L lactic acid total (based on 100-mL working volume) depending on the media used. Lactic acid is known to be a strong inhibitor for both growth and production, and it can be removed continuously by the adsorption on the PVP resin. With the fermenter-adsorber system, the fermentation can be performed as effectively as the ones with added neutralizing agents, such as calcium carbonate and sodium hydroxide. One problem of the fermenter-adsorber system is that lower production was obtained than in shake flasks; hence, proper reactor design is necessary to improve the process.},
doi = {},
url = {https://www.osti.gov/biblio/150395},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Dec 31 00:00:00 EST 1995},
month = {Sun Dec 31 00:00:00 EST 1995}
}