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Title: Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase

Authors:
; ; ; ; ; ; ORCiD logo; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
NSFNIH
OSTI Identifier:
1503697
Resource Type:
Journal Article
Journal Name:
Communications Biology
Additional Journal Information:
Journal Volume: 1; Journal Issue: 1; Journal ID: ISSN 2399-3642
Publisher:
Springer Nature
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Maheshwari, Sweta, Shimokawa, Chizu, Rudzka, Katarzyna, Kline, Chelsey D., Eipper, Betty A., Mains, Richard E., Gabelli, Sandra B., Blackburn, Ninian, and Amzel, L. Mario. Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase. United States: N. p., 2018. Web. doi:10.1038/s42003-018-0082-y.
Maheshwari, Sweta, Shimokawa, Chizu, Rudzka, Katarzyna, Kline, Chelsey D., Eipper, Betty A., Mains, Richard E., Gabelli, Sandra B., Blackburn, Ninian, & Amzel, L. Mario. Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase. United States. doi:10.1038/s42003-018-0082-y.
Maheshwari, Sweta, Shimokawa, Chizu, Rudzka, Katarzyna, Kline, Chelsey D., Eipper, Betty A., Mains, Richard E., Gabelli, Sandra B., Blackburn, Ninian, and Amzel, L. Mario. Mon . "Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase". United States. doi:10.1038/s42003-018-0082-y.
@article{osti_1503697,
title = {Effects of copper occupancy on the conformational landscape of peptidylglycine α-hydroxylating monooxygenase},
author = {Maheshwari, Sweta and Shimokawa, Chizu and Rudzka, Katarzyna and Kline, Chelsey D. and Eipper, Betty A. and Mains, Richard E. and Gabelli, Sandra B. and Blackburn, Ninian and Amzel, L. Mario},
abstractNote = {},
doi = {10.1038/s42003-018-0082-y},
journal = {Communications Biology},
issn = {2399-3642},
number = 1,
volume = 1,
place = {United States},
year = {2018},
month = {6}
}

Works referenced in this record:

Peptide amidation: Evidence for multiple molecular forms of the amidating enzyme
journal, August 1988

  • Bradbury, Alan F.; Smyth, Derek G.
  • Biochemical and Biophysical Research Communications, Vol. 154, Issue 3
  • DOI: 10.1016/0006-291X(88)90280-X

Peptide amidation
journal, January 1991


Peptidylglycine α-amidating monooxygenase: A multifunctional protein with catalytic, processing, and routing domains
journal, April 1993

  • Eipper, Betty A.; Milgram, Sharon L.; Jean Husten, E.
  • Protein Science, Vol. 2, Issue 4
  • DOI: 10.1002/pro.5560020401

New insights into copper monooxygenases and peptide amidation: structure, mechanism and function
journal, August 2000

  • Prigge, S. T.; Mains, R. E.; Eipper, B. A.
  • Cellular and Molecular Life Sciences, Vol. 57, Issue 8
  • DOI: 10.1007/PL00000763

Early eukaryotic origins for cilia-associated bioactive peptide-amidating activity
journal, January 2016

  • Kumar, Dhivya; Blaby-Haas, Crysten E.; Merchant, Sabeeha S.
  • Journal of Cell Science, Vol. 129, Issue 5
  • DOI: 10.1242/jcs.177410

A "Neural" Enzyme in Nonbilaterian Animals and Algae: Preneural Origins for Peptidylglycine  -Amidating Monooxygenase
journal, April 2012

  • Attenborough, R. M. F.; Hayward, D. C.; Kitahara, M. V.
  • Molecular Biology and Evolution, Vol. 29, Issue 10
  • DOI: 10.1093/molbev/mss114

Alternative mRNA splicing generates multiple forms of peptidyl-glycine alpha-amidating monooxygenase in rat atrium.
journal, January 1989

  • Stoffers, D. A.; Green, C. B.; Eipper, B. A.
  • Proceedings of the National Academy of Sciences, Vol. 86, Issue 2
  • DOI: 10.1073/pnas.86.2.735

Coordination of peroxide to the CuM center of peptidylglycine α-hydroxylating monooxygenase (PHM): structural and computational study
journal, December 2012

  • Rudzka, Katarzyna; Moreno, Diego M.; Eipper, Betty
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 18, Issue 2
  • DOI: 10.1007/s00775-012-0967-z

The Catalytic Copper of Peptidylglycine α-Hydroxylating Monooxygenase also Plays a Critical Structural Role
journal, November 2005


Differential Reactivity between Two Copper Sites in Peptidylglycine α-Hydroxylating Monooxygenase
journal, November 2010

  • Chufán, Eduardo E.; Prigge, Sean T.; Siebert, Xavier
  • Journal of the American Chemical Society, Vol. 132, Issue 44
  • DOI: 10.1021/ja103117r

The copper centers of tyramine β-monooxygenase and its catalytic-site methionine variants: an X-ray absorption study
journal, June 2010

  • Hess, Corinna R.; Klinman, Judith P.; Blackburn, Ninian J.
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 15, Issue 8
  • DOI: 10.1007/s00775-010-0677-3

Evidence That Dioxygen and Substrate Activation Are Tightly Coupled in Dopamine β-Monooxygenase: IMPLICATIONS FOR THE REACTIVE OXYGEN SPECIES
journal, September 2003

  • Evans, John P.; Ahn, Kyunghye; Klinman, Judith P.
  • Journal of Biological Chemistry, Vol. 278, Issue 50
  • DOI: 10.1074/jbc.M300797200

The Catalytic Role of the Copper Ligand H172 of Peptidylglycine α-Hydroxylating Monooxygenase:  A Kinetic Study of the H172A Mutant
journal, December 2006

  • Evans, John P.; Blackburn, Ninian J.; Klinman, Judith P.
  • Biochemistry, Vol. 45, Issue 51
  • DOI: 10.1021/bi061734c

Substituted hippurates and hippurate analogs as substrates and inhibitors of peptidylglycine α-hydroxylating monooxygenase (PHM)
journal, December 2008

  • Merkler, David J.; Asser, Alexander S.; Baumgart, Laura E.
  • Bioorganic & Medicinal Chemistry, Vol. 16, Issue 23
  • DOI: 10.1016/j.bmc.2008.10.013

Probing the Peptidylglycine α-Hydroxylating Monooxygenase Active Site with Novel 4-Phenyl-3-butenoic Acid Based Inhibitors
journal, August 2010


Hydroxylase Activity of Met471Cys Tyramine β-Monooxygenase
journal, September 2008

  • Hess, Corinna R.; Wu, Zinian; Ng, Adora
  • Journal of the American Chemical Society, Vol. 130, Issue 36
  • DOI: 10.1021/ja800408h

Isocyanide binding to the copper(I) centers of the catalytic core of peptidylglycine monooxygenase (PHMcc)
journal, May 2001

  • Rhames, Francis C.; Murthy, Narasimha N.; Karlin, Kenneth D.
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 6, Issue 5-6
  • DOI: 10.1007/s007750100233

X-ray Absorption Edge Spectroscopy and Computational Studies on LCuO 2 Species:  Superoxide−Cu II versus Peroxide−Cu III Bonding
journal, June 2006

  • Sarangi, Ritimukta; Aboelella, Nermeen; Fujisawa, Kiyoshi
  • Journal of the American Chemical Society, Vol. 128, Issue 25
  • DOI: 10.1021/ja0615223

Lumenal Loop M672-P707 of the Menkes Protein (ATP7A) Transfers Copper to Peptidylglycine Monooxygenase
journal, June 2012

  • Otoikhian, Adenike; Barry, Amanda N.; Mayfield, Mary
  • Journal of the American Chemical Society, Vol. 134, Issue 25
  • DOI: 10.1021/ja301221s

HHM Motif at the CuH-Site of Peptidylglycine Monooxygenase is a pH-Dependent Conformational Switch
journal, April 2013

  • Kline, Chelsey D.; Mayfield, Mary; Blackburn, Ninian J.
  • Biochemistry, Vol. 52, Issue 15
  • DOI: 10.1021/bi4002248

O2 activation by binuclear Cu sites: Noncoupled versus exchange coupled reaction mechanisms
journal, August 2004

  • Chen, P.; Solomon, E. I.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 36
  • DOI: 10.1073/pnas.0402114101

The Catalytic Mechanism of Peptidylglycine α-Hydroxylating Monooxygenase Investigated by Computer Simulation
journal, October 2006

  • Crespo, Alejandro; Martí, Marcelo A.; Roitberg, Adrian E.
  • Journal of the American Chemical Society, Vol. 128, Issue 39
  • DOI: 10.1021/ja062876x

Bifunctional Peptidylglycine α-Amidating Enzyme Requires 2 Copper Atoms for Maximum Activity
journal, May 1994

  • Kulathila, R.; Consalvo, A. P.; Fitzpatrick, P. F.
  • Archives of Biochemistry and Biophysics, Vol. 311, Issue 1
  • DOI: 10.1006/abbi.1994.1225

Peptidylglycine α-Hydroxylating Monooxygenase:  Active Site Residues, Disulfide Linkages, and a Two-Domain Model of the Catalytic Core
journal, September 1997

  • Kolhekar, Aparna S.; Keutmann, Henry T.; Mains, Richard E.
  • Biochemistry, Vol. 36, Issue 36
  • DOI: 10.1021/bi9708747

Identification of the Five Essential Histidine Residues for Peptidylglycine Monooxygenase
journal, January 1996

  • Yonekura, Hideto; Anzai, Takao; Kato, Ichiro
  • Biochemical and Biophysical Research Communications, Vol. 218, Issue 2
  • DOI: 10.1006/bbrc.1996.0088

Dioxygen Binds End-On to Mononuclear Copper in a Precatalytic Enzyme Complex
journal, May 2004


Identification in pituitary tissue of a peptide alpha-amidation activity that acts on glycine-extended peptides and requires molecular oxygen, copper, and ascorbic acid.
journal, August 1983

  • Eipper, B. A.; Mains, R. E.; Glembotski, C. C.
  • Proceedings of the National Academy of Sciences, Vol. 80, Issue 16
  • DOI: 10.1073/pnas.80.16.5144

Further Characterization of Peptidylglycine α-Amidating Monooxygenase from Bovine Neurointermediate Pituitary*
journal, April 1987

  • Murthy, Adavi S. N.; Keutmann, Henry T.; Eipper, Betty A.
  • Molecular Endocrinology, Vol. 1, Issue 4
  • DOI: 10.1210/mend-1-4-290

Crystallization of soluble proteins in vapor diffusion for x-ray crystallography
journal, June 2007


Plausible molecular mechanism for activation by fumarate and electron transfer of the dopamine β-mono-oxygenase reaction
journal, October 2002

  • Wimalasena, D. Shyamali; Jayatillake, Samantha P.; Haines, Donovan C.
  • Biochemical Journal, Vol. 367, Issue 1
  • DOI: 10.1042/bj20020216

The crystal structure of human dopamine β-hydroxylase at 2.9 Å resolution
journal, April 2016

  • Vendelboe, Trine V.; Harris, Pernille; Zhao, Yuguang
  • Science Advances, Vol. 2, Issue 4
  • DOI: 10.1126/sciadv.1500980

Hydrogen Tunneling in Peptidylglycine α-Hydroxylating Monooxygenase
journal, July 2002

  • Francisco, Wilson A.; Knapp, Michael J.; Blackburn, Ninian J.
  • Journal of the American Chemical Society, Vol. 124, Issue 28
  • DOI: 10.1021/ja025758s

Kinetic Mechanism and Intrinsic Isotope Effects for the Peptidylglycine α-Amidating Enzyme Reaction
journal, June 1998

  • Francisco, Wilson A.; Merkler, David J.; Blackburn, Ninian J.
  • Biochemistry, Vol. 37, Issue 22
  • DOI: 10.1021/bi973004y

Interdomain Long-Range Electron Transfer Becomes Rate-Limiting in the Y216A Variant of Tyramine β-Monooxygenase
journal, February 2013

  • Osborne, Robert L.; Zhu, Hui; Iavarone, Anthony T.
  • Biochemistry, Vol. 52, Issue 7
  • DOI: 10.1021/bi3013609

Supplying Copper to the Cuproenzyme Peptidylglycine α-Amidating Monooxygenase
journal, January 2003

  • El Meskini, Rajaâ; Culotta, Valeria Cizewski; Mains, Richard E.
  • Journal of Biological Chemistry, Vol. 278, Issue 14
  • DOI: 10.1074/jbc.M211413200

A Copper–Methionine Interaction Controls the pH-Dependent Activation of Peptidylglycine Monooxygenase
journal, December 2011

  • Bauman, Andrew T.; Broers, Brenda A.; Kline, Chelsey D.
  • Biochemistry, Vol. 50, Issue 50
  • DOI: 10.1021/bi201193j

The Catalytic Role of the Copper Ligand H172 of Peptidylglycine α-Hydroxylating Monooxygenase (PHM):  A Spectroscopic Study of the H172A Mutant
journal, November 2002

  • Jaron, Shulamit; Mains, Richard E.; Eipper, Betty A.
  • Biochemistry, Vol. 41, Issue 44
  • DOI: 10.1021/bi020404z

The Hydrogen Peroxide Reactivity of Peptidylglycine Monooxygenase Supports a Cu(II)-Superoxo Catalytic Intermediate
journal, January 2006


Metal Ions in Proteins: Structural and Functional Roles
journal, January 1987


pH-regulated metal–ligand switching in the HM loop of ATP7A: a new paradigm for metal transfer chemistry
journal, January 2016

  • Kline, Chelsey D.; Gambill, Benjamin F.; Mayfield, Mary
  • Metallomics, Vol. 8, Issue 8
  • DOI: 10.1039/C6MT00062B

Major changes in copper coordination accompany reduction of peptidylglycine monooxygenase: implications for electron transfer and the catalytic mechanism
journal, June 2000

  • Blackburn, Ninian J.; Rhames, Francis C.; Ralle, Martina
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 5, Issue 3
  • DOI: 10.1007/PL00010663

Large scale production of the copper enzyme peptidylglycine monooxygenase using an automated bioreactor
journal, January 2007

  • Bauman, Andrew T.; Ralle, Martina; Blackburn, Ninian J.
  • Protein Expression and Purification, Vol. 51, Issue 1
  • DOI: 10.1016/j.pep.2006.06.016

Features and development of Coot
journal, March 2010

  • Emsley, P.; Lohkamp, B.; Scott, W. G.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4
  • DOI: 10.1107/S0907444910007493

A graphical user interface to the CCP 4 program suite
journal, June 2003

  • Potterton, Elizabeth; Briggs, Peter; Turkenburg, Maria
  • Acta Crystallographica Section D Biological Crystallography, Vol. 59, Issue 7
  • DOI: 10.1107/S0907444903008126

Ongoing developments in CCP 4 for high-throughput structure determination
journal, October 2002

  • Winn, M. D.; Ashton, A. W.; Briggs, P. J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 58, Issue 11
  • DOI: 10.1107/S0907444902016116

MolProbity : all-atom structure validation for macromolecular crystallography
journal, December 2009

  • Chen, Vincent B.; Arendall, W. Bryan; Headd, Jeffrey J.
  • Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 1
  • DOI: 10.1107/S0907444909042073