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Title: Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS

Abstract

We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was used to deposit cells into a nanodroplet system for proteomic sample preparation. After processing, samples were analysed by ultrasensitive nanoLC-MS. Using this platform, an average of ~670 protein groups were identified from single HeLa cells and ~330 proteins were quantifiable. We demonstrated the single cell proteomics platform can be used to differentiate cell types from enzyme-dissociated primary cells from human lung and identify specific protein marker among them.

Authors:
ORCiD logo [1]; ORCiD logo [2];  [2];  [2];  [1]; ORCiD logo [2];  [2];  [3]; ORCiD logo [3]; ORCiD logo [2]; ORCiD logo [2]; ORCiD logo [1]
  1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland WA 99354 USA
  2. Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA 99354 USA
  3. Department of Pediatrics-Neonatology, University of Rochester Medical Center, Rochester NY 14642 USA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1503622
Report Number(s):
PNNL-SA-132946
Journal ID: ISSN 1433-7851
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Angewandte Chemie (International Edition)
Additional Journal Information:
Journal Volume: 57; Journal Issue: 38; Journal ID: ISSN 1433-7851
Publisher:
Wiley
Country of Publication:
United States
Language:
English

Citation Formats

Zhu, Ying, Clair, Geremy, Chrisler, William B., Shen, Yufeng, Zhao, Rui, Shukla, Anil K., Moore, Ronald J., Misra, Ravi S., Pryhuber, Gloria S., Smith, Richard D., Ansong, Charles, and Kelly, Ryan T. Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS. United States: N. p., 2018. Web. doi:10.1002/anie.201802843.
Zhu, Ying, Clair, Geremy, Chrisler, William B., Shen, Yufeng, Zhao, Rui, Shukla, Anil K., Moore, Ronald J., Misra, Ravi S., Pryhuber, Gloria S., Smith, Richard D., Ansong, Charles, & Kelly, Ryan T. Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS. United States. doi:10.1002/anie.201802843.
Zhu, Ying, Clair, Geremy, Chrisler, William B., Shen, Yufeng, Zhao, Rui, Shukla, Anil K., Moore, Ronald J., Misra, Ravi S., Pryhuber, Gloria S., Smith, Richard D., Ansong, Charles, and Kelly, Ryan T. Thu . "Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS". United States. doi:10.1002/anie.201802843.
@article{osti_1503622,
title = {Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS},
author = {Zhu, Ying and Clair, Geremy and Chrisler, William B. and Shen, Yufeng and Zhao, Rui and Shukla, Anil K. and Moore, Ronald J. and Misra, Ravi S. and Pryhuber, Gloria S. and Smith, Richard D. and Ansong, Charles and Kelly, Ryan T.},
abstractNote = {We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was used to deposit cells into a nanodroplet system for proteomic sample preparation. After processing, samples were analysed by ultrasensitive nanoLC-MS. Using this platform, an average of ~670 protein groups were identified from single HeLa cells and ~330 proteins were quantifiable. We demonstrated the single cell proteomics platform can be used to differentiate cell types from enzyme-dissociated primary cells from human lung and identify specific protein marker among them.},
doi = {10.1002/anie.201802843},
journal = {Angewandte Chemie (International Edition)},
issn = {1433-7851},
number = 38,
volume = 57,
place = {United States},
year = {2018},
month = {6}
}

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