Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS

Zhu, Ying; Clair, Geremy; Chrisler, William B.; Shen, Yufeng; Zhao, Rui; Shukla, Anil K.; Moore, Ronald J.; Misra, Ravi S.; Pryhuber, Gloria S.; Smith, Richard D.; Ansong, Charles; Kelly, Ryan T.

doi:10.1002/anie.201802843

Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS

Journal Article · 14 June 2018 · Angewandte Chemie (International Edition)

DOI:https://doi.org/10.1002/anie.201802843· OSTI ID:1503622

^[1]; ^[2]; Chrisler, William B. ^[2]; Shen, Yufeng ^[2]; Zhao, Rui ^[1]; ^[2]; Moore, Ronald J. ^[2]; Misra, Ravi S. ^[3]; ^[3]; ^[2]; ^[2]; ^[1]

Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland WA 99354 USA
Biological Sciences Division, Pacific Northwest National Laboratory, Richland WA 99354 USA
Department of Pediatrics-Neonatology, University of Rochester Medical Center, Rochester NY 14642 USA

We report on the quantitative proteomic analysis of single mammalian cells. Fluorescence-activated cell sorting was used to deposit cells into a nanodroplet system for proteomic sample preparation. After processing, samples were analysed by ultrasensitive nanoLC-MS. Using this platform, an average of ~670 protein groups were identified from single HeLa cells and ~330 proteins were quantifiable. We demonstrated the single cell proteomics platform can be used to differentiate cell types from enzyme-dissociated primary cells from human lung and identify specific protein marker among them.

Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1503622

Report Number(s):: PNNL-SA-132946

Journal Information:: Angewandte Chemie (International Edition), Vol. 57, Issue 38; ISSN 1433-7851

Publisher:: Wiley

Country of Publication:: United States

Language:: English

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