Cell type-resolved human lung lipidome reveals cellular cooperation in lung function

Kyle, Jennifer E.; Clair, Geremy; Bandyopadhyay, Gautam; Misra, Ravi S.; Zink, Erika M.; Bloodsworth, Kent J.; Shukla, Anil K.; Du, Yina; Lillis, Jacquelyn; Myers, Jason R.; Ashton, John; Bushnell, Timothy; Cochran, Matthew; Deutsch, Gail; Baker, Erin S.; Carson, James P.; Mariani, Thomas J.; Xu, Yan; Whitsett, Jeffrey A.; Pryhuber, Gloria; Ansong, Charles

doi:10.1038/s41598-018-31640-x

Title: Cell type-resolved human lung lipidome reveals cellular cooperation in lung function

Journal Article · Fri Sep 07 00:00:00 EDT 2018 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-018-31640-x· OSTI ID:1503561

Kyle, Jennifer E.; Clair, Geremy; Bandyopadhyay, Gautam; Misra, Ravi S.; Zink, Erika M.; Bloodsworth, Kent J.; Shukla, Anil K.; Du, Yina; Lillis, Jacquelyn;

; Ashton, John; Bushnell, Timothy; Cochran, Matthew; Deutsch, Gail; Baker, Erin S.; Carson, James P.; Mariani, Thomas J.; Xu, Yan; Whitsett, Jeffrey A.; Pryhuber, Gloria more »

Cell type-resolved proteome analyses of the brain, heart and liver have been reported, however a similar effort on the lipidome is currently lacking. Here we applied liquid chromatography-tandem mass spectrometry to characterize the lipidome of major lung cell types isolated from human donors, representing the first lipidome map of any organ. We coupled this with cell type-resolved proteomics of the same samples (available at Lungmap.net). Complementary proteomics analyses substantiated the functional identity of the isolated cells. Lipidomics analyses showed significant variations in the lipidome across major human lung cell types, with differences most evident at the subclass and intra-subclass (i.e. total carbon length of the fatty acid chains) level. Further, lipidomic signatures revealed an overarching posture of high cellular cooperation within the human lung to support critical functions. Our complementary cell type-resolved lipid and protein datasets serve as a rich resource for analyses of human lung function.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1503561

Report Number(s):: PNNL-SA-132404

Journal Information:: Scientific Reports, Vol. 8, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing Group

Country of Publication:: United States

Language:: English

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