Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells

Dou, Maowei; Zhu, Ying; Liyu, Andrey; Liang, Yiran; Chen, Jing; Piehowski, Paul D.; Xu, Kerui; Zhao, Rui; Moore, Ronald J.; Atkinson, Mark A.; Mathews, Clayton E.; Qian, Wei-Jun; Kelly, Ryan T.

doi:10.1039/C8SC02680G

Title: Nanowell-mediated two-dimensional liquid chromatography enables deep proteome profiling of <1000 mammalian cells

Journal Article · Mon Jan 01 00:00:00 EST 2018 · Chemical Science

DOI:https://doi.org/10.1039/C8SC02680G· OSTI ID:1503526

^[1];

^[1]; Liyu, Andrey ^[1]; Liang, Yiran ^[1]; Chen, Jing ^[2]; Piehowski, Paul D. ^[3]; Xu, Kerui ^[1]; Zhao, Rui ^[1]; Moore, Ronald J. ^[3]; Atkinson, Mark A. ^[2]; Mathews, Clayton E. ^[2];

^[3];

^[1]

Environmental Molecular Sciences Laboratory; Pacific Northwest National Laboratory; Richland; USA
Department of Pathology; Immunology and Laboratory Medicine; University of Florida; Gainesville; USA
Biological Sciences Division; Pacific Northwest National Laboratory; Richland; USA

Miniaturized multidimensional peptide separations can greatly increase the coverage in proteome profiling for trace samples.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1503526

Report Number(s):: PNNL-SA-133217; CSHCBM

Journal Information:: Chemical Science, Vol. 9, Issue 34; ISSN 2041-6520

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

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Proteome analysis of tissues by mass spectrometry Dapic, Irena; Baljeu‐Neuman, Lucia; Uwugiaren, Naomi Mass Spectrometry Reviews, Vol. 38, Issue 4-5 https://doi.org/10.1002/mas.21598	journal	August 2019
Nanowell-mediated multidimensional separations combining nanoLC with SLIM IM-MS for rapid, high-peak-capacity proteomic analyses Dou, Maowei; Chouinard, Christopher D.; Zhu, Ying Analytical and Bioanalytical Chemistry, Vol. 411, Issue 21 https://doi.org/10.1007/s00216-018-1452-5	journal	November 2018
New mass spectrometry technologies contributing towards comprehensive and high throughput omics analyses of single cells Couvillion, Sneha P.; Zhu, Ying; Nagy, Gabe The Analyst, Vol. 144, Issue 3 https://doi.org/10.1039/c8an01574k	journal	January 2019
The role of proteomics in assessing beta-cell dysfunction and death in type 1 diabetes Nakayasu, Ernesto S.; Qian, Wei-Jun; Evans-Molina, Carmella Expert Review of Proteomics, Vol. 16, Issue 7 https://doi.org/10.1080/14789450.2019.1634548	journal	June 2019

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