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Title: Salinivirga fredricksonii gen. nov., sp. nov., a heterotrophic halophile isolated from a photosynthetic mat, a member of a novel lineage (Salinarimonadaceae fam. nov.) within the order Rhizobiales, and reclassification of the genus Salinarimonas Liu et al. 2010 into Salinarimonadaceae

Abstract

A halophilic bacterial strain, HL-109T, was isolated from the unicyanobacterial consortium UCC-O, which was obtained from the photosynthetic mat of Hot Lake (Washington, USA). A polyphasic approach using phenotypic, genotypic, and chemotaxonomic data was used to classify the strain within the order “Rhizobiales”. The organism stains Gram negative and is a moderate thermophile with an optimum of 45 °C. It is an obligate aerobe, heterotrophic, and a halophile that grows in both NaCl and MgSO4 brines. The novel isolate has a polymorphic cellular morphology of short rods with occasional branching and cells were monotrichous. The major fatty acids detected were C18:1, C18:0, C16:0, and C18:cyc. Phylogenetic analysis of the 16S rRNA gene placed the strain in the order “Rhizobiales” and it shares 94% identity to its nearest relative, Salinarimonas ramus. Morphological, chemotaxonomic, and phylogenetic results did not affiliate the novel organism with any of the families in the “Rhizobiales”; therefore, HL-109T is representative of a new lineage, which we propose to be Salinivirga fredricksonii gen. nov, sp. nov., Salinivirgaceae fam. nov., with the type strain of Salinivirga fredricksonii gen. nov. sp. nov. as HL-109T (JCM TBDT and DSMZ TBDT). In addition, examination of the phylogenetics of the strain HL-109T andmore » its nearest relatives, Salinarimonas ramus and S. rosea, demonstrate that these halophiles form a clade distinct from the families of the “Rhizobiales”. We thus recommend transferring the genus Salinarimonas to join the novel family Salinivirgaceae.« less

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [6];  [7];  [8];  [6];  [6];  [6];  [9]
  1. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 2​Scientific and Computing Operations, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA
  2. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 3​Risk and Decision Sciences, Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
  3. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; †​Present address: Center for Infectious Disease Research, Seattle, WA, USA.
  4. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
  5. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 5​Environmental Dynamics and Simulations, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA
  6. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
  7. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; ‡​Present address: U.S. Naval Research Laboratory, Washington, DC, USA.
  8. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; §​Present address: Nova Research, Inc., Alexandria, VA, USA.
  9. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 6​Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN, USA; 7​Department of Nutrition Science, Purdue University, West Lafayette, IN, USA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1503504
Report Number(s):
PNNL-SA-118460
Journal ID: ISSN 1466-5026
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
International Journal of Systematic and Evolutionary Microbiology
Additional Journal Information:
Journal Volume: 68; Journal Issue: 5; Journal ID: ISSN 1466-5026
Publisher:
International Union of Microbiological Societies
Country of Publication:
United States
Language:
English

Citation Formats

Cole, Jessica K., Morton, Beau R., Cardamone, Hayley C., Lake, Hannah R. R., Dohnalkova, Alice C., Kim, Young-Mo, Kyle, Jennifer E., Maezato, Yukari, Dana, Karl L., Metz, Thomas O., Romine, Margaret F., Nelson, William C., and Lindemann, Stephen R. Salinivirga fredricksonii gen. nov., sp. nov., a heterotrophic halophile isolated from a photosynthetic mat, a member of a novel lineage (Salinarimonadaceae fam. nov.) within the order Rhizobiales, and reclassification of the genus Salinarimonas Liu et al. 2010 into Salinarimonadaceae. United States: N. p., 2018. Web. doi:10.1099/ijsem.0.002715.
Cole, Jessica K., Morton, Beau R., Cardamone, Hayley C., Lake, Hannah R. R., Dohnalkova, Alice C., Kim, Young-Mo, Kyle, Jennifer E., Maezato, Yukari, Dana, Karl L., Metz, Thomas O., Romine, Margaret F., Nelson, William C., & Lindemann, Stephen R. Salinivirga fredricksonii gen. nov., sp. nov., a heterotrophic halophile isolated from a photosynthetic mat, a member of a novel lineage (Salinarimonadaceae fam. nov.) within the order Rhizobiales, and reclassification of the genus Salinarimonas Liu et al. 2010 into Salinarimonadaceae. United States. doi:10.1099/ijsem.0.002715.
Cole, Jessica K., Morton, Beau R., Cardamone, Hayley C., Lake, Hannah R. R., Dohnalkova, Alice C., Kim, Young-Mo, Kyle, Jennifer E., Maezato, Yukari, Dana, Karl L., Metz, Thomas O., Romine, Margaret F., Nelson, William C., and Lindemann, Stephen R. Tue . "Salinivirga fredricksonii gen. nov., sp. nov., a heterotrophic halophile isolated from a photosynthetic mat, a member of a novel lineage (Salinarimonadaceae fam. nov.) within the order Rhizobiales, and reclassification of the genus Salinarimonas Liu et al. 2010 into Salinarimonadaceae". United States. doi:10.1099/ijsem.0.002715.
@article{osti_1503504,
title = {Salinivirga fredricksonii gen. nov., sp. nov., a heterotrophic halophile isolated from a photosynthetic mat, a member of a novel lineage (Salinarimonadaceae fam. nov.) within the order Rhizobiales, and reclassification of the genus Salinarimonas Liu et al. 2010 into Salinarimonadaceae},
author = {Cole, Jessica K. and Morton, Beau R. and Cardamone, Hayley C. and Lake, Hannah R. R. and Dohnalkova, Alice C. and Kim, Young-Mo and Kyle, Jennifer E. and Maezato, Yukari and Dana, Karl L. and Metz, Thomas O. and Romine, Margaret F. and Nelson, William C. and Lindemann, Stephen R.},
abstractNote = {A halophilic bacterial strain, HL-109T, was isolated from the unicyanobacterial consortium UCC-O, which was obtained from the photosynthetic mat of Hot Lake (Washington, USA). A polyphasic approach using phenotypic, genotypic, and chemotaxonomic data was used to classify the strain within the order “Rhizobiales”. The organism stains Gram negative and is a moderate thermophile with an optimum of 45 °C. It is an obligate aerobe, heterotrophic, and a halophile that grows in both NaCl and MgSO4 brines. The novel isolate has a polymorphic cellular morphology of short rods with occasional branching and cells were monotrichous. The major fatty acids detected were C18:1, C18:0, C16:0, and C18:cyc. Phylogenetic analysis of the 16S rRNA gene placed the strain in the order “Rhizobiales” and it shares 94% identity to its nearest relative, Salinarimonas ramus. Morphological, chemotaxonomic, and phylogenetic results did not affiliate the novel organism with any of the families in the “Rhizobiales”; therefore, HL-109T is representative of a new lineage, which we propose to be Salinivirga fredricksonii gen. nov, sp. nov., Salinivirgaceae fam. nov., with the type strain of Salinivirga fredricksonii gen. nov. sp. nov. as HL-109T (JCM TBDT and DSMZ TBDT). In addition, examination of the phylogenetics of the strain HL-109T and its nearest relatives, Salinarimonas ramus and S. rosea, demonstrate that these halophiles form a clade distinct from the families of the “Rhizobiales”. We thus recommend transferring the genus Salinarimonas to join the novel family Salinivirgaceae.},
doi = {10.1099/ijsem.0.002715},
journal = {International Journal of Systematic and Evolutionary Microbiology},
issn = {1466-5026},
number = 5,
volume = 68,
place = {United States},
year = {2018},
month = {5}
}