Salinivirga fredricksonii gen. nov., sp. nov., a heterotrophic halophile isolated from a photosynthetic mat, a member of a novel lineage (Salinarimonadaceae fam. nov.) within the order Rhizobiales, and reclassification of the genus Salinarimonas Liu et al. 2010 into Salinarimonadaceae

Journal Article · Tue May 01 00:00:00 EDT 2018 · International Journal of Systematic and Evolutionary Microbiology

DOI:https://doi.org/10.1099/ijsem.0.002715· OSTI ID:1503504

Cole, Jessica K. ^[1]; Morton, Beau R. ^[2]; Cardamone, Hayley C. ^[3]; Lake, Hannah R. R. ^[4]; Dohnalkova, Alice C. ^[5]; Kim, Young-Mo ^[6]; Kyle, Jennifer E. ^[6]; Maezato, Yukari ^[7]; Dana, Karl L. ^[8]; Metz, Thomas O. ^[6]; Romine, Margaret F. ^[6]; Nelson, William C. ^[6]; Lindemann, Stephen R. ^[9]

---

1. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 2​Scientific and Computing Operations, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA
2. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 3​Risk and Decision Sciences, Energy and Environment Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
3. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; †​Present address: Center for Infectious Disease Research, Seattle, WA, USA.
4. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
5. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 5​Environmental Dynamics and Simulations, Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA
6. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA
7. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; ‡​Present address: U.S. Naval Research Laboratory, Washington, DC, USA.
8. 4​Chemical, Biological, and Physical Sciences Division, National Security Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; §​Present address: Nova Research, Inc., Alexandria, VA, USA.
9. 1​Biological Sciences Division, Fundamental and Computational Sciences Directorate, Pacific Northwest National Laboratory, Richland, WA, USA; 6​Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, West Lafayette, IN, USA; 7​Department of Nutrition Science, Purdue University, West Lafayette, IN, USA

A halophilic bacterial strain, HL-109T, was isolated from the unicyanobacterial consortium UCC-O, which was obtained from the photosynthetic mat of Hot Lake (Washington, USA). A polyphasic approach using phenotypic, genotypic, and chemotaxonomic data was used to classify the strain within the order “Rhizobiales”. The organism stains Gram negative and is a moderate thermophile with an optimum of 45 °C. It is an obligate aerobe, heterotrophic, and a halophile that grows in both NaCl and MgSO4 brines. The novel isolate has a polymorphic cellular morphology of short rods with occasional branching and cells were monotrichous. The major fatty acids detected were C18:1, C18:0, C16:0, and C18:cyc. Phylogenetic analysis of the 16S rRNA gene placed the strain in the order “Rhizobiales” and it shares 94% identity to its nearest relative, Salinarimonas ramus. Morphological, chemotaxonomic, and phylogenetic results did not affiliate the novel organism with any of the families in the “Rhizobiales”; therefore, HL-109T is representative of a new lineage, which we propose to be Salinivirga fredricksonii gen. nov, sp. nov., Salinivirgaceae fam. nov., with the type strain of Salinivirga fredricksonii gen. nov. sp. nov. as HL-109T (JCM TBDT and DSMZ TBDT). In addition, examination of the phylogenetics of the strain HL-109T and its nearest relatives, Salinarimonas ramus and S. rosea, demonstrate that these halophiles form a clade distinct from the families of the “Rhizobiales”. We thus recommend transferring the genus Salinarimonas to join the novel family Salinivirgaceae.

• Research Organization: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 1503504
• Report Number(s): PNNL-SA-118460
• Journal Information: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, Issue 5; ISSN 1466-5026
• Publisher: International Union of Microbiological Societies
• Country of Publication: United States
• Language: English