Short papers on current state of sequencing, metagenomics, and RNAseq for diagnostics
This report contains three papers on the current state of sequencing, metagenomics, and RNAseq for diagnostics. 1) There are many factors that must be evaluated before ascertaining what type of technology is most cost effective, and/or most technically effective. In general, long read technologies are primarily used for in field/in situ sequencing and for closing isolate genomes, while short read sequencing lends itself to rapid high quality determination of strain identity and to applications that require high depth, such as metagenomes and counting applications, like in RNAseq. 2) RNAseq can provide some important insights into the biology of active infections and provide advances in diagnostics approaches. Depth of sequencing coverage is critical to determine gene expression profiles. Full use of RNAseq data from both host and pathogen(s) for diagnostics and informed treatment options will require further investigation. 3) RNAseq can provide some important insights into the biology of active infections and provide advances in diagnostics approaches. Depth of sequencing coverage is critical to determine gene expression profiles. Full use of RNAseq data from both host and pathogen(s) for diagnostics and informed treatment options will require further investigation.
- Research Organization:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Organization:
- Defense Threat Reduction Agency (DTRA)
- DOE Contract Number:
- 89233218CNA000001
- OSTI ID:
- 1503174
- Report Number(s):
- LA-UR-19-22515
- Country of Publication:
- United States
- Language:
- English
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