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Title: Transcriptome analysis of rubber biosynthesis in guayule (Parthenium argentatum gray)

Abstract

BACKGROUND: Natural rubber is currently produced nearly exclusively from latex of the Para rubber tree, Hevea brasiliensis. The desire to reduce the environmental cost of rubber production, fears of pathogen susceptibility in clonal Hevea plantations, volatility in the price of natural rubber, and increasing labor costs have motivated efforts to diversify the supply of natural rubber by developing alternative rubber crops such as guayule (Parthenium argentatum Gray). In Hevea, latex is produced as an exudate following wounding while in guayule, rubber is deposited within the cortical parenchyma and its production is strongly influenced by environmental conditions. RESULTS: To better understand the enzymology and regulation of guayule rubber biosynthesis and to identify genes with potential uses in the improvement of rubber yields, we conducted de novo transcriptome assembly and differential gene expression analyses of this process in guayule. This analysis supports a role for rubber in the defense against pathogens, identified new enzymes potentially involved in the biosynthesis of rubber as well as transcription factors specifically expressed in rubber-producing tissues. CONCLUSIONS: Data presented here will be useful in the improvement of guayule as an alternative source of natural rubber and in better understanding the biosynthesis of this critical polymer. In particular,more » some of the candidate transcription factors are likely to control the rubber biosynthesis pathway and are good targets for molecular breeding or engineering of guayule plants with higher and more consistent production of rubber.« less

Authors:
; ORCiD logo
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1502858
Alternate Identifier(s):
OSTI ID: 1498691
Report Number(s):
LBNL-CRADA-2001189
Journal ID: ISSN 1471-2229; ark:/13030/qt7qb3z866
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
BMC Plant Biology
Additional Journal Information:
Journal Volume: 19; Journal Issue: 1; Journal ID: ISSN 1471-2229
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Stonebloom, Solomon H., and Scheller, Henrik Vibe. Transcriptome analysis of rubber biosynthesis in guayule (Parthenium argentatum gray). United States: N. p., 2019. Web. doi:10.1186/s12870-019-1669-2.
Stonebloom, Solomon H., & Scheller, Henrik Vibe. Transcriptome analysis of rubber biosynthesis in guayule (Parthenium argentatum gray). United States. doi:10.1186/s12870-019-1669-2.
Stonebloom, Solomon H., and Scheller, Henrik Vibe. Tue . "Transcriptome analysis of rubber biosynthesis in guayule (Parthenium argentatum gray)". United States. doi:10.1186/s12870-019-1669-2. https://www.osti.gov/servlets/purl/1502858.
@article{osti_1502858,
title = {Transcriptome analysis of rubber biosynthesis in guayule (Parthenium argentatum gray)},
author = {Stonebloom, Solomon H. and Scheller, Henrik Vibe},
abstractNote = {BACKGROUND: Natural rubber is currently produced nearly exclusively from latex of the Para rubber tree, Hevea brasiliensis. The desire to reduce the environmental cost of rubber production, fears of pathogen susceptibility in clonal Hevea plantations, volatility in the price of natural rubber, and increasing labor costs have motivated efforts to diversify the supply of natural rubber by developing alternative rubber crops such as guayule (Parthenium argentatum Gray). In Hevea, latex is produced as an exudate following wounding while in guayule, rubber is deposited within the cortical parenchyma and its production is strongly influenced by environmental conditions. RESULTS: To better understand the enzymology and regulation of guayule rubber biosynthesis and to identify genes with potential uses in the improvement of rubber yields, we conducted de novo transcriptome assembly and differential gene expression analyses of this process in guayule. This analysis supports a role for rubber in the defense against pathogens, identified new enzymes potentially involved in the biosynthesis of rubber as well as transcription factors specifically expressed in rubber-producing tissues. CONCLUSIONS: Data presented here will be useful in the improvement of guayule as an alternative source of natural rubber and in better understanding the biosynthesis of this critical polymer. In particular, some of the candidate transcription factors are likely to control the rubber biosynthesis pathway and are good targets for molecular breeding or engineering of guayule plants with higher and more consistent production of rubber.},
doi = {10.1186/s12870-019-1669-2},
journal = {BMC Plant Biology},
issn = {1471-2229},
number = 1,
volume = 19,
place = {United States},
year = {2019},
month = {2}
}

Journal Article:
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Figures / Tables:

Figure 1 Figure 1: Verification of rubber biosynthesis induction in guayule plants by exposure to simulated winter conditions for 2 months. Washed rubber particle extractions from plants grown in simulated summer (a) and winter (b) conditions. c Quantitative RT-PCR of rubber biosynthetic enzymes (AOS, alllene oxide synthase; CPT, cis prenyl transferase; FPS,more » farnesyl diphosphate synthase; SRPP, small rubber particle protein). Significant differences between induced and control are shown (t-test, * p < 0.01, ** p < 0.001)« less

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Works referenced in this record:

Transcriptome and gene expression analysis in cold-acclimated guayule (Parthenium argentatum) rubber-producing tissue
journal, July 2012


    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.