Discovery of Macrocyclic Inhibitors of Apurinic/Apyrimidinic Endonuclease 1
- Boston Univ., MA (United States). Center for Molecular Discovery (BU-CMD)
- Boston Univ., MA (United States)
- Indiana Univ., Indianapolis, IN (United States). School of Medicine
- Indiana Univ., Indianapolis, IN (United States). School of Medicine, Herman B. Wells Center for Pediatric Research
- Boston Univ., MA (United States). Center for Molecular Discovery (BU-CMD); Emory Univ., Atlanta, GA (United States)
- Indiana Univ., Indianapolis, IN (United States). School of Medicine; Purdue Univ., Indianapolis, IN (United States). Purdue School of Science
Apurinic/apyrimidinic endonuclease 1 (APE1) is an essential base excision repair enzyme that is upregulated in a number of cancers, contributes to resistance of tumors treated with DNA-alkylating or -oxidizing agents, and has recently been identified as an important therapeutic target. Here in this work, we identified hot spots for binding of small organic molecules experimentally in high resolution crystal structures of APE1 and computationally through the use of FTMAP analysis (http://ftmap.bu.edu/). Guided by these hot spots, a library of drug-like macrocycles was docked and then screened for inhibition of APE1 endonuclease activity. In an iterative process, hot-spot-guided docking, characterization of inhibition of APE1 endonuclease, and cytotoxicity of cancer cells were used to design next generation macrocycles. To assess target selectivity in cells, selected macrocycles were analyzed for modulation of DNA damage. Taken together, our studies suggest that macrocycles represent a promising class of compounds for inhibition of APE1 in cancer cells.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- National Institutes of Health (NIH); Earl and Betty Herr Professor in Pediatric Oncology Research; Jeff Gordon Children’s Foundation; Riley Children’s Foundation; USDOE
- Grant/Contract Number:
- R01CA205166; R01CA167291; R24 GM111625; R35 GM118078
- OSTI ID:
- 1502255
- Journal Information:
- Journal of Medicinal Chemistry, Vol. 62, Issue 4; ISSN 0022-2623
- Publisher:
- American Chemical Society (ACS)Copyright Statement
- Country of Publication:
- United States
- Language:
- ENGLISH
Web of Science
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