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Title: High-resolution crystal structure of Streptococcus agalactiae glyceraldehyde-3-phosphate dehydrogenase

Abstract

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme that plays critical roles in bacterial pathogenesis in some pathogenic bacteria. In this study, the crystal structure of group B streptococcus GAPDH was determined at 1.36 Å resolution. The structure contained an asymmetric mixed holo tetramer, with two NAD ligands bound to two protomers. Further structural analysis identified interesting phosphate ion-binding sites, which shed light on its catalytic mechanism.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
FOREIGN
OSTI Identifier:
1502235
Resource Type:
Journal Article
Journal Name:
Acta Crystallographica. Section F, Structural Biology Communications
Additional Journal Information:
Journal Volume: 74; Journal Issue: 4; Journal ID: ISSN 2053-230X
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

Zhou, Kang, Fan, Xiaojiao, Li, Yuelong, Zhang, Caiying, and Jin, Tengchuan. High-resolution crystal structure of Streptococcus agalactiae glyceraldehyde-3-phosphate dehydrogenase. United States: N. p., 2018. Web. doi:10.1107/S2053230X18003801.
Zhou, Kang, Fan, Xiaojiao, Li, Yuelong, Zhang, Caiying, & Jin, Tengchuan. High-resolution crystal structure of Streptococcus agalactiae glyceraldehyde-3-phosphate dehydrogenase. United States. doi:10.1107/S2053230X18003801.
Zhou, Kang, Fan, Xiaojiao, Li, Yuelong, Zhang, Caiying, and Jin, Tengchuan. Fri . "High-resolution crystal structure of Streptococcus agalactiae glyceraldehyde-3-phosphate dehydrogenase". United States. doi:10.1107/S2053230X18003801.
@article{osti_1502235,
title = {High-resolution crystal structure of Streptococcus agalactiae glyceraldehyde-3-phosphate dehydrogenase},
author = {Zhou, Kang and Fan, Xiaojiao and Li, Yuelong and Zhang, Caiying and Jin, Tengchuan},
abstractNote = {Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme that plays critical roles in bacterial pathogenesis in some pathogenic bacteria. In this study, the crystal structure of group B streptococcus GAPDH was determined at 1.36 Å resolution. The structure contained an asymmetric mixed holo tetramer, with two NAD ligands bound to two protomers. Further structural analysis identified interesting phosphate ion-binding sites, which shed light on its catalytic mechanism.},
doi = {10.1107/S2053230X18003801},
journal = {Acta Crystallographica. Section F, Structural Biology Communications},
issn = {2053-230X},
number = 4,
volume = 74,
place = {United States},
year = {2018},
month = {3}
}