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Title: Enzyme Nanoparticles-Based Electronic Biosensor

Abstract

A novel method for fabricating electronic biosensors based on coupling enzyme nanoparticles and self assembly technology is illustrated. Redox horseradish peroxidase nanoparticles were prepared by desolvation with ethanol and subsequent crosslinking with glutaraldehyde. The cross-linked enzyme nanoparticles were functionalized by cysteine to introduce thiol groups on the nanoparticle surface. Immobilized enzyme nanoparticle on the gold electrode by self-assembly kept redox and electrocatalytic activities, and was used to develop reagentless biosensors for H2O2 detection without promoters and mediators. The new approach is simple, low cost and circumvents complications associated with solution systems. It is a universal immobilization method for biosensor, biomedical devices, biofuel cells and enzymatic bioreactors fabrication and expected to open new opportunities for biosensor, clinical diagnostics, and for bioanalysis, in general.

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
15016607
Report Number(s):
PNNL-SA-44063
6899; TRN: US200515%%280
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Chemical Communications, 2005(27):3481-3483
Additional Journal Information:
Journal Volume: 2005; Journal Issue: 27
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; BIOFUELS; BIOREACTORS; CYSTEINE; DETECTION; ELECTRODES; ENZYMES; ETHANOL; FABRICATION; GOLD; IMMOBILIZED ENZYMES; PEROXIDASES; PROMOTERS; THIOLS; Biosensor; Environmental Molecular Sciences Laboratory

Citation Formats

Liu, Guodong, Lin, Yuehe, Ostatna, V, and Wang, Joseph. Enzyme Nanoparticles-Based Electronic Biosensor. United States: N. p., 2005. Web. doi:10.1039/b504943a.
Liu, Guodong, Lin, Yuehe, Ostatna, V, & Wang, Joseph. Enzyme Nanoparticles-Based Electronic Biosensor. United States. doi:10.1039/b504943a.
Liu, Guodong, Lin, Yuehe, Ostatna, V, and Wang, Joseph. Tue . "Enzyme Nanoparticles-Based Electronic Biosensor". United States. doi:10.1039/b504943a.
@article{osti_15016607,
title = {Enzyme Nanoparticles-Based Electronic Biosensor},
author = {Liu, Guodong and Lin, Yuehe and Ostatna, V and Wang, Joseph},
abstractNote = {A novel method for fabricating electronic biosensors based on coupling enzyme nanoparticles and self assembly technology is illustrated. Redox horseradish peroxidase nanoparticles were prepared by desolvation with ethanol and subsequent crosslinking with glutaraldehyde. The cross-linked enzyme nanoparticles were functionalized by cysteine to introduce thiol groups on the nanoparticle surface. Immobilized enzyme nanoparticle on the gold electrode by self-assembly kept redox and electrocatalytic activities, and was used to develop reagentless biosensors for H2O2 detection without promoters and mediators. The new approach is simple, low cost and circumvents complications associated with solution systems. It is a universal immobilization method for biosensor, biomedical devices, biofuel cells and enzymatic bioreactors fabrication and expected to open new opportunities for biosensor, clinical diagnostics, and for bioanalysis, in general.},
doi = {10.1039/b504943a},
journal = {Chemical Communications, 2005(27):3481-3483},
number = 27,
volume = 2005,
place = {United States},
year = {2005},
month = {6}
}