A preliminary time-of-flight neutron diffraction study on amicyanin from paracoccus denitrificans.

Journal Article · Sun May 01 00:00:00 EDT 2005 · Acta Crystallographica Section D

DOI:https://doi.org/10.1107/S090744490500541X· OSTI ID:15016532

Sukumar, N; Langan, P; Mathews, F S; Jones, L H; Thiyagarajan, P; Schoenborn, B P; Davidson, V L; ASO,; IPNS,

Crystals of the blue copper protein amicyanin suitable for neutron diffraction were grown by the sitting-drop method, followed by repeated macroseeding using solutions prepared with D{sub 2}O. Although the crystal sizes were the same, crystals grown using solutions made up in H{sub 2}O in the initial stages of macroseeding and solutions with D{sub 2}O in later stages did not diffract neutrons well. However, when the protein was initially exchanged with buffered D{sub 2}O and then crystallized and also macroseeded using solutions made up in D{sub 2}O throughout, the crystals diffracted neutrons to high resolution. One of those crystals was used to collect a data set to a resolution of 1.9 {angstrom}.

• Research Organization: Intense Pulsed Neutron Source, Argonne National Laboratory, Argonne, IL (US); LANL; Washington Univ. School of Medicine; Univ. of Mississippi Medical Center (US)
• Sponsoring Organization: US Department of Energy (US)
• DOE Contract Number: W-31-109-ENG-38
• OSTI ID: 15016532
• Journal Information: Acta Crystallographica Section D, Vol. 61, Issue 5; Other Information: PBD: May 2005; Related Information: May 2005
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS
COPPER
NEUTRON DIFFRACTION
NEUTRONS
PROTEINS
RESOLUTION
INTENSE PULSED NEUTRON SOURCE