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Title: Efficient Cytosolic Delivery Using Crystalline Nanoflowers Assembled from Fluorinated Peptoids

Abstract

The design and synthesis of biocompatible nanomaterials as cargoes for the intracellular delivery of therapeutic proteins or genes have attracted intense attention because of their promise for use in therapeutics. Despite the advances in this area, very few nanomaterials can be efficiently delivered to the cytosol. To address these challenges, we designed and synthesized crystalline nanoflower-like particles from fluorinated sequence-defined peptoids; the crystallinity and fluorination of these particles enabled highly efficient cytosolic delivery with minimal cytotoxicity. We delivered these particles, 80% appear to diffuse in the cytosol. Furthermore, these nanocrystals can carry mRNA for instance and effectively delivered into cytosol for gene transfection, demonstrating the generality of our nanocrystals. Our results indicate that self-assembly of crystalline nanomaterials from fluorinated peptoids paves a new way toward development of nanocargoes with efficient cytosol gene delivery. Y. Song and M. Wang contributed equally to this work. This work was supported by start-up funds from Washington State University. Peptoid synthesis work was supported by the Materials Synthesis and Simulation Across Scales (MS3) Initiative through the LDRD fund at Pacific Northwest National Laboratory (PNNL). Assembly of peptoid particles and their structural characterizations were supported by the U.S. Department of Energy, Office of Basic Energy Sciences,more » Biomolecular Materials Program at PNNL. XRD work was conducted at the Advanced Light Source with support from the Molecular Foundry, at Lawrence Berkeley National Laboratory, both of which are supported by the Office of Science, under Contrast No. DE-AC02-05CH11231. PNNL is multi-program national laboratory operated for Department of Energy by Battelle under Contract No. DE-AC05-76RL01830.« less

Authors:
 [1];  [2];  [1];  [2];  [1];  [1];  [1];  [2]; ORCiD logo [1]
  1. Department of Mechanical and Materials Engineering, Washington State University, Pullman WA 99164 USA
  2. Division of Physical Sciences, Pacific Northwest National Laboratory, Richland WA 99352 USA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1501614
Report Number(s):
PNNL-SA-139291
Journal ID: ISSN 1613-6810
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Small
Additional Journal Information:
Journal Volume: 14; Journal Issue: 52; Journal ID: ISSN 1613-6810
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
peptoids, nanocrystal, cytosol delivery, endosome escape, minimal cytotoxicity

Citation Formats

Song, Yang, Wang, Mingming, Li, Suiqiong, Jin, Haibao, Cai, Xiaoli, Du, Dan, Li, He, Chen, Chun-Long, and Lin, Yuehe. Efficient Cytosolic Delivery Using Crystalline Nanoflowers Assembled from Fluorinated Peptoids. United States: N. p., 2018. Web. doi:10.1002/smll.201803544.
Song, Yang, Wang, Mingming, Li, Suiqiong, Jin, Haibao, Cai, Xiaoli, Du, Dan, Li, He, Chen, Chun-Long, & Lin, Yuehe. Efficient Cytosolic Delivery Using Crystalline Nanoflowers Assembled from Fluorinated Peptoids. United States. doi:10.1002/smll.201803544.
Song, Yang, Wang, Mingming, Li, Suiqiong, Jin, Haibao, Cai, Xiaoli, Du, Dan, Li, He, Chen, Chun-Long, and Lin, Yuehe. Thu . "Efficient Cytosolic Delivery Using Crystalline Nanoflowers Assembled from Fluorinated Peptoids". United States. doi:10.1002/smll.201803544.
@article{osti_1501614,
title = {Efficient Cytosolic Delivery Using Crystalline Nanoflowers Assembled from Fluorinated Peptoids},
author = {Song, Yang and Wang, Mingming and Li, Suiqiong and Jin, Haibao and Cai, Xiaoli and Du, Dan and Li, He and Chen, Chun-Long and Lin, Yuehe},
abstractNote = {The design and synthesis of biocompatible nanomaterials as cargoes for the intracellular delivery of therapeutic proteins or genes have attracted intense attention because of their promise for use in therapeutics. Despite the advances in this area, very few nanomaterials can be efficiently delivered to the cytosol. To address these challenges, we designed and synthesized crystalline nanoflower-like particles from fluorinated sequence-defined peptoids; the crystallinity and fluorination of these particles enabled highly efficient cytosolic delivery with minimal cytotoxicity. We delivered these particles, 80% appear to diffuse in the cytosol. Furthermore, these nanocrystals can carry mRNA for instance and effectively delivered into cytosol for gene transfection, demonstrating the generality of our nanocrystals. Our results indicate that self-assembly of crystalline nanomaterials from fluorinated peptoids paves a new way toward development of nanocargoes with efficient cytosol gene delivery. Y. Song and M. Wang contributed equally to this work. This work was supported by start-up funds from Washington State University. Peptoid synthesis work was supported by the Materials Synthesis and Simulation Across Scales (MS3) Initiative through the LDRD fund at Pacific Northwest National Laboratory (PNNL). Assembly of peptoid particles and their structural characterizations were supported by the U.S. Department of Energy, Office of Basic Energy Sciences, Biomolecular Materials Program at PNNL. XRD work was conducted at the Advanced Light Source with support from the Molecular Foundry, at Lawrence Berkeley National Laboratory, both of which are supported by the Office of Science, under Contrast No. DE-AC02-05CH11231. PNNL is multi-program national laboratory operated for Department of Energy by Battelle under Contract No. DE-AC05-76RL01830.},
doi = {10.1002/smll.201803544},
journal = {Small},
issn = {1613-6810},
number = 52,
volume = 14,
place = {United States},
year = {2018},
month = {11}
}