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Title: How radiation kills cells: Survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress

Abstract

The radiation resistant bacterium Deinococcus radiodurans accumulates very high intracellular manganese and relatively low iron levels compared to the dissimilatory metal-reducing bacterium Shewanella oneidensis which is extremely sensitive. For Fe-rich, Mn-poor cells, death at low doses might be caused by the release of Fe(II) from proteins during irradiation, followed by Fe(II)-dependent reduction of hydrogen peroxide produced by metabolism after irradiation. In contrast, Mn(II) ions concentrated in D. radiodurans might serve as antioxidants that reinforce enzymic systems which defend against oxidative stress during recovery. We extend our hypothesis here to include consideration of respiration, tricarboxylic acid cycle activity, peptide transport, and metal reduction, which together with Mn(II) transport represent potential new targets to control cell recovery from radiation injury.

Authors:
; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab., Richland, WA (US), Environmental Molecular Sciences Laboratory (US)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
15015965
Report Number(s):
PNNL-SA-43797
10199; KP1301010; TRN: US200509%%524
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
FEMS Microbiology Reviews
Additional Journal Information:
Journal Volume: 29; Journal Issue: 2; Other Information: PBD: 1 Apr 2005
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ANTIOXIDANTS; DEATH; HYDROGEN PEROXIDE; HYPOTHESIS; IRON; IRRADIATION; MANGANESE; METABOLISM; PEPTIDES; PROTEINS; RADIATIONS; RESPIRATION; TARGETS; TRANSPORT; ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY

Citation Formats

Ghosal, D, Omelchenko, M V, Gaidamakova, E, Matrosova, V, Vasilenko, A, Venkateswaran, Amudhan, Zhai, M, Kostandarithes, Heather M, Brim, Hassan, Makarova, Kira S, Wackett, L P, Fredrickson, Jim K, and Daly, Michael J. How radiation kills cells: Survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. United States: N. p., 2005. Web. doi:10.1016/j.femsre.2004.12.007.
Ghosal, D, Omelchenko, M V, Gaidamakova, E, Matrosova, V, Vasilenko, A, Venkateswaran, Amudhan, Zhai, M, Kostandarithes, Heather M, Brim, Hassan, Makarova, Kira S, Wackett, L P, Fredrickson, Jim K, & Daly, Michael J. How radiation kills cells: Survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress. United States. https://doi.org/10.1016/j.femsre.2004.12.007
Ghosal, D, Omelchenko, M V, Gaidamakova, E, Matrosova, V, Vasilenko, A, Venkateswaran, Amudhan, Zhai, M, Kostandarithes, Heather M, Brim, Hassan, Makarova, Kira S, Wackett, L P, Fredrickson, Jim K, and Daly, Michael J. 2005. "How radiation kills cells: Survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress". United States. https://doi.org/10.1016/j.femsre.2004.12.007.
@article{osti_15015965,
title = {How radiation kills cells: Survival of Deinococcus radiodurans and Shewanella oneidensis under oxidative stress},
author = {Ghosal, D and Omelchenko, M V and Gaidamakova, E and Matrosova, V and Vasilenko, A and Venkateswaran, Amudhan and Zhai, M and Kostandarithes, Heather M and Brim, Hassan and Makarova, Kira S and Wackett, L P and Fredrickson, Jim K and Daly, Michael J},
abstractNote = {The radiation resistant bacterium Deinococcus radiodurans accumulates very high intracellular manganese and relatively low iron levels compared to the dissimilatory metal-reducing bacterium Shewanella oneidensis which is extremely sensitive. For Fe-rich, Mn-poor cells, death at low doses might be caused by the release of Fe(II) from proteins during irradiation, followed by Fe(II)-dependent reduction of hydrogen peroxide produced by metabolism after irradiation. In contrast, Mn(II) ions concentrated in D. radiodurans might serve as antioxidants that reinforce enzymic systems which defend against oxidative stress during recovery. We extend our hypothesis here to include consideration of respiration, tricarboxylic acid cycle activity, peptide transport, and metal reduction, which together with Mn(II) transport represent potential new targets to control cell recovery from radiation injury.},
doi = {10.1016/j.femsre.2004.12.007},
url = {https://www.osti.gov/biblio/15015965}, journal = {FEMS Microbiology Reviews},
number = 2,
volume = 29,
place = {United States},
year = {Fri Apr 01 00:00:00 EST 2005},
month = {Fri Apr 01 00:00:00 EST 2005}
}