Recovery and Visualization of 3D Structure of Chromosomes from Tomographic Reconstruction Images
Abstract
The objectives of this work include automatic recovery and visualization of a 3D chromosome structure from a sequence of 2D tomographic reconstruction images taken through the nucleus of a cell. Structure is very important for biologists as it affects chromosome functions, behavior of the cell and its state. Chromosome analysis is significant in the detection of deceases and in monitoring environmental gene mutations. The algorithm incorporates thresholding based on a histogram analysis with a polyline splitting algorithm, contour extraction via active contours, and detection of the 3D chromosome structure by establishing corresponding regions throughout the slices. Visualization using point cloud meshing generates a 3D surface. The 3D triangular mesh of the chromosomes provides surface detail and allows a user to interactively analyze chromosomes using visualization software.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 15014143
- Report Number(s):
- UCRL-PROC-203893
Journal ID: ISSN 1687-6180; TRN: US200805%%308
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Conference
- Resource Relation:
- Journal Volume: 2006; Journal Issue: 1; Conference: Presented at: IEEE Workshop on Articulated and Nonrigid, Washington, DC, United States, Jun 27 - Jun 27, 2004
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 42 ENGINEERING; 99 GENERAL AND MISCELLANEOUS//MATHEMATICS, COMPUTING, AND INFORMATION SCIENCE; 59 BASIC BIOLOGICAL SCIENCES; ALGORITHMS; CHROMOSOMES; DETECTION; GENE MUTATIONS; MONITORING
Citation Formats
Babu, S, Liao, P, Shin, M C, and Tsap, L V. Recovery and Visualization of 3D Structure of Chromosomes from Tomographic Reconstruction Images. United States: N. p., 2004.
Web. doi:10.1155/ASP/2006/45684.
Babu, S, Liao, P, Shin, M C, & Tsap, L V. Recovery and Visualization of 3D Structure of Chromosomes from Tomographic Reconstruction Images. United States. https://doi.org/10.1155/ASP/2006/45684
Babu, S, Liao, P, Shin, M C, and Tsap, L V. 2004.
"Recovery and Visualization of 3D Structure of Chromosomes from Tomographic Reconstruction Images". United States. https://doi.org/10.1155/ASP/2006/45684. https://www.osti.gov/servlets/purl/15014143.
@article{osti_15014143,
title = {Recovery and Visualization of 3D Structure of Chromosomes from Tomographic Reconstruction Images},
author = {Babu, S and Liao, P and Shin, M C and Tsap, L V},
abstractNote = {The objectives of this work include automatic recovery and visualization of a 3D chromosome structure from a sequence of 2D tomographic reconstruction images taken through the nucleus of a cell. Structure is very important for biologists as it affects chromosome functions, behavior of the cell and its state. Chromosome analysis is significant in the detection of deceases and in monitoring environmental gene mutations. The algorithm incorporates thresholding based on a histogram analysis with a polyline splitting algorithm, contour extraction via active contours, and detection of the 3D chromosome structure by establishing corresponding regions throughout the slices. Visualization using point cloud meshing generates a 3D surface. The 3D triangular mesh of the chromosomes provides surface detail and allows a user to interactively analyze chromosomes using visualization software.},
doi = {10.1155/ASP/2006/45684},
url = {https://www.osti.gov/biblio/15014143},
journal = {},
issn = {1687-6180},
number = 1,
volume = 2006,
place = {United States},
year = {Wed Apr 28 00:00:00 EDT 2004},
month = {Wed Apr 28 00:00:00 EDT 2004}
}
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