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Title: Tailored Noise Waveform/ Collision-Induced Dissociation of Ions Stored in a Linear Ion Trap Combined with Liquid Chromatography/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry

Abstract

A new collision-induced dissociation (CID) technique based on broadband tailored noise waveform (TNW) excitation of ions stored in a linear ion trap has been developed. In comparison with the conventional sustained off-resonance irradiation (SORI) CID method commonly used in Fourier transform ion cyclotron resonance mass spectrometry, this MS/MS technique increases throughput by eliminating the long pump-down delay associated with gas introduction into the high vacuum ICR cell region. In addition, the TNW-CID method speeds spectrum acquisition since it does not require Fourier transformation, calculation of resonant frequencies and generation of the excitation waveforms. We demonstrate TNW-CID coupled with on-line capillary reverse phase liquid chromatography separations for identification of peptides. The experimental results are compared with data obtained using conventional quadrupole ion trap MS/MS and SORI-CID MS/MS in an ICR cell.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
15011378
Report Number(s):
PNNL-SA-42697
Journal ID: ISSN 0951-4198; RCMSEF; KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Rapid Communications in Mass Spectrometry, 18(22):2682-2690; Journal Volume: 18; Journal Issue: 22
Country of Publication:
United States
Language:
English

Citation Formats

Vilkov, Andrey N., Bogdanov, Bogdan, Pasa-Tolic, Liljiana, Prior, David C., Anderson, Gordon A., Masselon, Christophe D., Moore, Ronald J., and Smith, Richard D. Tailored Noise Waveform/ Collision-Induced Dissociation of Ions Stored in a Linear Ion Trap Combined with Liquid Chromatography/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. United States: N. p., 2004. Web. doi:10.1002/rcm.1664.
Vilkov, Andrey N., Bogdanov, Bogdan, Pasa-Tolic, Liljiana, Prior, David C., Anderson, Gordon A., Masselon, Christophe D., Moore, Ronald J., & Smith, Richard D. Tailored Noise Waveform/ Collision-Induced Dissociation of Ions Stored in a Linear Ion Trap Combined with Liquid Chromatography/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry. United States. doi:10.1002/rcm.1664.
Vilkov, Andrey N., Bogdanov, Bogdan, Pasa-Tolic, Liljiana, Prior, David C., Anderson, Gordon A., Masselon, Christophe D., Moore, Ronald J., and Smith, Richard D. Mon . "Tailored Noise Waveform/ Collision-Induced Dissociation of Ions Stored in a Linear Ion Trap Combined with Liquid Chromatography/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry". United States. doi:10.1002/rcm.1664.
@article{osti_15011378,
title = {Tailored Noise Waveform/ Collision-Induced Dissociation of Ions Stored in a Linear Ion Trap Combined with Liquid Chromatography/Fourier Transform Ion Cyclotron Resonance Mass Spectrometry},
author = {Vilkov, Andrey N. and Bogdanov, Bogdan and Pasa-Tolic, Liljiana and Prior, David C. and Anderson, Gordon A. and Masselon, Christophe D. and Moore, Ronald J. and Smith, Richard D.},
abstractNote = {A new collision-induced dissociation (CID) technique based on broadband tailored noise waveform (TNW) excitation of ions stored in a linear ion trap has been developed. In comparison with the conventional sustained off-resonance irradiation (SORI) CID method commonly used in Fourier transform ion cyclotron resonance mass spectrometry, this MS/MS technique increases throughput by eliminating the long pump-down delay associated with gas introduction into the high vacuum ICR cell region. In addition, the TNW-CID method speeds spectrum acquisition since it does not require Fourier transformation, calculation of resonant frequencies and generation of the excitation waveforms. We demonstrate TNW-CID coupled with on-line capillary reverse phase liquid chromatography separations for identification of peptides. The experimental results are compared with data obtained using conventional quadrupole ion trap MS/MS and SORI-CID MS/MS in an ICR cell.},
doi = {10.1002/rcm.1664},
journal = {Rapid Communications in Mass Spectrometry, 18(22):2682-2690},
number = 22,
volume = 18,
place = {United States},
year = {Mon Nov 01 00:00:00 EST 2004},
month = {Mon Nov 01 00:00:00 EST 2004}
}