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Title: On the Relative Strengths of Bacillus Spore Signatures in the Terahertz (THz)Versus the Mid-Infrared

Abstract

We have recently extended our studies of the infrared signatures of Bacillus bacterial spores from the mid-infrared to the far-infrared (sometimes called the terahertz, THz) spectral domain. The ultimate goal is to use such signatures to distinguish different strains of spores from unknowns as well as from one another. Five different strains of Bacillus were prepared by culturing the spores, washing repeatedly in sterile water and drying them onto windows that are simultaneously transparent in both the mid- and far-infrared. The strains include B. globigii BG-01, B. thuringiensis subsp kurstaki ATCC 35866, B. subtilis ATCC 49760, B. subtilis ATCC 6051, and B. atrophaeus ATCC 49337. Using different combinations of hardware in the Fourier transform infrared (FTIR) spectrometer, essentially continuous spectral coverage was obtained from {approx}8 to 6,000 cm-1. Preliminary results indicate that any THz signatures are at least 25 times weaker (based on p-p noise) than the strongest mid-IR amide I bands near 1657 cm-1.

Authors:
; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab., Richland, WA (US), Environmental Molecular Sciences Laboratory (US)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
15011343
Report Number(s):
PNNL-SA-42979
5116; KP1301030; TRN: US200506%%262
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: Proceedings of the SPIE, Conference location not supplied, Conference dates not supplied; Other Information: PBD: 27 Oct 2004
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMIDES; BACILLUS; BACTERIAL SPORES; DRYING; SPORES; STRAINS; WASHING; WATER; WINDOWS; ENVIRONMENTAL MOLECULAR SCIENCES LABORATORY

Citation Formats

Valentine, Nancy B, Johnson, Timothy J, and Sharpe, Steven W. On the Relative Strengths of Bacillus Spore Signatures in the Terahertz (THz)Versus the Mid-Infrared. United States: N. p., 2004. Web.
Valentine, Nancy B, Johnson, Timothy J, & Sharpe, Steven W. On the Relative Strengths of Bacillus Spore Signatures in the Terahertz (THz)Versus the Mid-Infrared. United States.
Valentine, Nancy B, Johnson, Timothy J, and Sharpe, Steven W. 2004. "On the Relative Strengths of Bacillus Spore Signatures in the Terahertz (THz)Versus the Mid-Infrared". United States.
@article{osti_15011343,
title = {On the Relative Strengths of Bacillus Spore Signatures in the Terahertz (THz)Versus the Mid-Infrared},
author = {Valentine, Nancy B and Johnson, Timothy J and Sharpe, Steven W},
abstractNote = {We have recently extended our studies of the infrared signatures of Bacillus bacterial spores from the mid-infrared to the far-infrared (sometimes called the terahertz, THz) spectral domain. The ultimate goal is to use such signatures to distinguish different strains of spores from unknowns as well as from one another. Five different strains of Bacillus were prepared by culturing the spores, washing repeatedly in sterile water and drying them onto windows that are simultaneously transparent in both the mid- and far-infrared. The strains include B. globigii BG-01, B. thuringiensis subsp kurstaki ATCC 35866, B. subtilis ATCC 49760, B. subtilis ATCC 6051, and B. atrophaeus ATCC 49337. Using different combinations of hardware in the Fourier transform infrared (FTIR) spectrometer, essentially continuous spectral coverage was obtained from {approx}8 to 6,000 cm-1. Preliminary results indicate that any THz signatures are at least 25 times weaker (based on p-p noise) than the strongest mid-IR amide I bands near 1657 cm-1.},
doi = {},
url = {https://www.osti.gov/biblio/15011343}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Oct 27 00:00:00 EDT 2004},
month = {Wed Oct 27 00:00:00 EDT 2004}
}

Conference:
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