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Title: Development of Biosensors Based on Carbon Nanotube Nanoelectrode Arrays

Abstract

The fabrication, electrochemical characterization, and sensing applications of low-site density carbon nanotubes based nanoelectrode arrays (CNT-NEAs) are reported in this work. Spin-coating of an epoxy resin provides a new way to create the electrode passivation layer that effectively reduces the current leakage and eliminates the electrode capacitance by sealing the side-wall of CNTs. The CNT-NEAs fabricated in our work effectively use the open ends of CNTs for electrochemical sensing. The open ends of the CNTs have fast electron transfer rates similar to a graphite edge-plane electrode, while the side-walls present very slow electron transfer rates similar to the graphitic basal plane. Cyclic voltammetry showed the sigmoidal shape curves with low capacitive current and scan-rate-independent limiting current. The successful development of a glucose biosensor based on CNT-NEAs for the selective detection of glucose is also described. Glucose oxidase was covalently immobilized on the CNTs tips via carbodiimide chemistry by forming amide linkages between the amine residues and carboxylic acid groups on the open ends of CNTs. The biosensor effectively performs selective electrochemical detections of glucose in the presence of common interferences.

Authors:
; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
15011246
Report Number(s):
PNNL-SA-43264
6899
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Conference
Resource Relation:
Conference: Nanosensing: Materials and Devices. Proceedings of SPIE , 5593(44-51
Country of Publication:
United States
Language:
English
Subject:
Carbon nanotubes; biosensor; Environmental Molecular Sciences Laboratory

Citation Formats

Lin, Yuehe, Tu, Yi, Lu, Fang, and Ren, Zhifeng. Development of Biosensors Based on Carbon Nanotube Nanoelectrode Arrays. United States: N. p., 2004. Web.
Lin, Yuehe, Tu, Yi, Lu, Fang, & Ren, Zhifeng. Development of Biosensors Based on Carbon Nanotube Nanoelectrode Arrays. United States.
Lin, Yuehe, Tu, Yi, Lu, Fang, and Ren, Zhifeng. Tue . "Development of Biosensors Based on Carbon Nanotube Nanoelectrode Arrays". United States.
@article{osti_15011246,
title = {Development of Biosensors Based on Carbon Nanotube Nanoelectrode Arrays},
author = {Lin, Yuehe and Tu, Yi and Lu, Fang and Ren, Zhifeng},
abstractNote = {The fabrication, electrochemical characterization, and sensing applications of low-site density carbon nanotubes based nanoelectrode arrays (CNT-NEAs) are reported in this work. Spin-coating of an epoxy resin provides a new way to create the electrode passivation layer that effectively reduces the current leakage and eliminates the electrode capacitance by sealing the side-wall of CNTs. The CNT-NEAs fabricated in our work effectively use the open ends of CNTs for electrochemical sensing. The open ends of the CNTs have fast electron transfer rates similar to a graphite edge-plane electrode, while the side-walls present very slow electron transfer rates similar to the graphitic basal plane. Cyclic voltammetry showed the sigmoidal shape curves with low capacitive current and scan-rate-independent limiting current. The successful development of a glucose biosensor based on CNT-NEAs for the selective detection of glucose is also described. Glucose oxidase was covalently immobilized on the CNTs tips via carbodiimide chemistry by forming amide linkages between the amine residues and carboxylic acid groups on the open ends of CNTs. The biosensor effectively performs selective electrochemical detections of glucose in the presence of common interferences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {12}
}

Conference:
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