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Title: Comparison of the Microbial Community Composition at Yucca Mountain and Laboratory Test Nuclear Repository Environments

Conference ·
OSTI ID:15004036

The microbiological community structure within a proposed nuclear waste repository at Yucca Mountain (YM), NV was determined. Microbial growth from collected rock was detected using simulated ground water as a growth medium, with or without amendment of a carbon source. Grown isolates were identified by 16s ribosomal DNA (rDNA) sequence analysis. A more complete compositional analysis of the microbial community located at the proposed nuclear waste repository site was performed using environmental DNA isolation and subsequent identification of amplified 16s rDNA genes. Concurrently, a series of corrosion testing tanks that simulate the evolution of anticipated environmental conditions within the proposed repository have been subjected to the same type of analyses.

Research Organization:
Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48
OSTI ID:
15004036
Report Number(s):
UCRL-JC-150693; TRN: US1005112
Resource Relation:
Conference: National Association of Corrosion Expo 2003 Oceans of Opportunity Corrosion International Annual Meeting, San Diego, CA, Mar 16 - Mar 20, 2003
Country of Publication:
United States
Language:
English