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Title: Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility

Abstract

Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking so we studied a candidate gene that we identified within the BAHD acyl-CoA transferase family. We used RNA interference (RNAi) silencing of orthologs in the model grasses Setaria viridis (SvBAHD01) and Brachypodium distachyon (BdBAHD01) and determined effects on AX feruloylation. Silencing of SvBAHD01 in Setaria resulted in a c. 60% decrease in AX feruloylation in stems consistently across four generations. Silencing of BdBAHD01 in Brachypodium stems decreased feruloylation much less, possibly due to higher expression of functionally redundant genes. Setaria SvBAHD01 RNAi plants showed: no decrease in total lignin, approximately doubled arabinose acylated by p-coumarate, changes in two-dimensional NMR spectra of unfractionated cell walls consistent with biochemical estimates, no effect on total biomass production and an increase in biomass saccharification efficiency of 40–60%. We provide the first strong evidence for a key role of the BAHD01 gene in AX feruloylation and demonstrate that it is a promising target for improvement of grass crops for biofuel, biorefining and animal nutrition applications.

Authors:
 [1];  [1];  [2];  [2];  [2];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];  [3];  [3];  [3];  [3];  [4];  [5];  [6] more »;  [7];  [7];  [7];  [8]; ORCiD logo [2];  [1] « less
  1. Embrapa Agroenergy, Brasília (Brazil)
  2. Rothamsted Research, Harpenden (United Kingdom)
  3. State Univ. of Maringá (Brazil)
  4. Brazilian Center for Research in Energy and Materials, Sao Paulo (Brazil)
  5. Universidade de Sorocaba (UNISO) (Brazil)
  6. Federal Univ. of ABC (Brazil)
  7. Inst. of Botany, Sao Paulo (Brazil)
  8. Univ. of Wisconsin, Madison, WI (United States)
Publication Date:
Research Org.:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1500216
Grant/Contract Number:  
FC02-07ER64494
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
New Phytologist
Additional Journal Information:
Journal Volume: 218; Journal Issue: 1; Journal ID: ISSN 0028-646X
Publisher:
Wiley
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

de Souza, Wagner R., Martins, Polyana K., Freeman, Jackie, Pellny, Till K., Michaelson, Louise V., Sampaio, Bruno L., Vinecky, Felipe, Ribeiro, Ana P., da Cunha, Barbara A. D. B., Kobayashi, Adilson K., de Oliveira, Patricia A., Campanha, Raquel B., Pacheco, Thályta F., Martarello, Danielly C. I., Marchiosi, Rogério, Ferrarese-Filho, Osvaldo, dos Santos, Wanderley D., Tramontina, Robson, Squina, Fabio M., Centeno, Danilo C., Gaspar, Marília, Braga, Marcia R., Tiné, Marco A. S., Ralph, John, Mitchell, Rowan A. C., and Molinari, Hugo B. C. Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility. United States: N. p., 2018. Web. doi:10.1111/nph.14970.
de Souza, Wagner R., Martins, Polyana K., Freeman, Jackie, Pellny, Till K., Michaelson, Louise V., Sampaio, Bruno L., Vinecky, Felipe, Ribeiro, Ana P., da Cunha, Barbara A. D. B., Kobayashi, Adilson K., de Oliveira, Patricia A., Campanha, Raquel B., Pacheco, Thályta F., Martarello, Danielly C. I., Marchiosi, Rogério, Ferrarese-Filho, Osvaldo, dos Santos, Wanderley D., Tramontina, Robson, Squina, Fabio M., Centeno, Danilo C., Gaspar, Marília, Braga, Marcia R., Tiné, Marco A. S., Ralph, John, Mitchell, Rowan A. C., & Molinari, Hugo B. C. Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility. United States. doi:10.1111/nph.14970.
de Souza, Wagner R., Martins, Polyana K., Freeman, Jackie, Pellny, Till K., Michaelson, Louise V., Sampaio, Bruno L., Vinecky, Felipe, Ribeiro, Ana P., da Cunha, Barbara A. D. B., Kobayashi, Adilson K., de Oliveira, Patricia A., Campanha, Raquel B., Pacheco, Thályta F., Martarello, Danielly C. I., Marchiosi, Rogério, Ferrarese-Filho, Osvaldo, dos Santos, Wanderley D., Tramontina, Robson, Squina, Fabio M., Centeno, Danilo C., Gaspar, Marília, Braga, Marcia R., Tiné, Marco A. S., Ralph, John, Mitchell, Rowan A. C., and Molinari, Hugo B. C. Mon . "Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility". United States. doi:10.1111/nph.14970. https://www.osti.gov/servlets/purl/1500216.
@article{osti_1500216,
title = {Suppression of a single BAHD gene in Setaria viridis causes large, stable decreases in cell wall feruloylation and increases biomass digestibility},
author = {de Souza, Wagner R. and Martins, Polyana K. and Freeman, Jackie and Pellny, Till K. and Michaelson, Louise V. and Sampaio, Bruno L. and Vinecky, Felipe and Ribeiro, Ana P. and da Cunha, Barbara A. D. B. and Kobayashi, Adilson K. and de Oliveira, Patricia A. and Campanha, Raquel B. and Pacheco, Thályta F. and Martarello, Danielly C. I. and Marchiosi, Rogério and Ferrarese-Filho, Osvaldo and dos Santos, Wanderley D. and Tramontina, Robson and Squina, Fabio M. and Centeno, Danilo C. and Gaspar, Marília and Braga, Marcia R. and Tiné, Marco A. S. and Ralph, John and Mitchell, Rowan A. C. and Molinari, Hugo B. C.},
abstractNote = {Feruloylation of arabinoxylan (AX) in grass cell walls is a key determinant of recalcitrance to enzyme attack, making it a target for improvement of grass crops, and of interest in grass evolution. Definitive evidence on the genes responsible is lacking so we studied a candidate gene that we identified within the BAHD acyl-CoA transferase family. We used RNA interference (RNAi) silencing of orthologs in the model grasses Setaria viridis (SvBAHD01) and Brachypodium distachyon (BdBAHD01) and determined effects on AX feruloylation. Silencing of SvBAHD01 in Setaria resulted in a c. 60% decrease in AX feruloylation in stems consistently across four generations. Silencing of BdBAHD01 in Brachypodium stems decreased feruloylation much less, possibly due to higher expression of functionally redundant genes. Setaria SvBAHD01 RNAi plants showed: no decrease in total lignin, approximately doubled arabinose acylated by p-coumarate, changes in two-dimensional NMR spectra of unfractionated cell walls consistent with biochemical estimates, no effect on total biomass production and an increase in biomass saccharification efficiency of 40–60%. We provide the first strong evidence for a key role of the BAHD01 gene in AX feruloylation and demonstrate that it is a promising target for improvement of grass crops for biofuel, biorefining and animal nutrition applications.},
doi = {10.1111/nph.14970},
journal = {New Phytologist},
issn = {0028-646X},
number = 1,
volume = 218,
place = {United States},
year = {2018},
month = {1}
}

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