skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Surface-induced dissociation in a Fourier transform ion cyclotron resonance mass spectrometer: Instrument design and evaluation

Abstract

A new Fourier Transform Ion Cyclotron Resonance mass spectrometer (FT-ICR MS) has been constructed in our laboratory. The instrument employs surface-induced dissociation (SID) as an activation method for obtaining structural information on biomolecules in the gas phase. Tandem SID mass spectra can be acquired using either a continuous or a pulsed mode of operation. Collision energy of precursor ion is controlled by a dc offset of the ICR cell. This approach eliminates defocusing of the ion beam by the ion transfer optics as a function of ion kinetic energy and constitutes a significant improvement over our previous experimental setup. Furthermore, it can be easily implemented on any FT-ICR mass spectrometer. Very high signal-to-noise ratios of 200-500 were obtained in single-scan SID mass spectra of model peptides with acquisition time less than 1.1 s. Reasonable SID signal was detected in single-scan spectra with total acquisition time of only 0.3 s. The high signal-to-noise ratio and the fast acquisition time point on a potential application of SID for high-throughput studies in FT-ICR MS.

Authors:
 [1];  [2];  [1];  [1];  [1]
  1. (BATTELLE (PACIFIC NW LAB))
  2. (University of Delaware)
Publication Date:
Research Org.:
Pacific Northwest National Lab., Richland, WA (US)
Sponsoring Org.:
US Department of Energy (US)
OSTI Identifier:
15001088
Report Number(s):
PNNL-SA-35829
TRN: US200401%%406
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry; Journal Volume: 74; Other Information: PBD: 1 Dec 2001
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; DESIGN; DISSOCIATION; EVALUATION; ION CYCLOTRON-RESONANCE; MASS SPECTRA; FOURIER TRANSFORM SPECTROMETERS; PEPTIDES; SIGNAL-TO-NOISE RATIO; OPERATION; PERFORMANCE

Citation Formats

Laskin, Julia, Denisov, Eduard, Shukla, Anil K., Barlow, Stephan E., and Futrell, Jean H. Surface-induced dissociation in a Fourier transform ion cyclotron resonance mass spectrometer: Instrument design and evaluation. United States: N. p., 2001. Web.
Laskin, Julia, Denisov, Eduard, Shukla, Anil K., Barlow, Stephan E., & Futrell, Jean H. Surface-induced dissociation in a Fourier transform ion cyclotron resonance mass spectrometer: Instrument design and evaluation. United States.
Laskin, Julia, Denisov, Eduard, Shukla, Anil K., Barlow, Stephan E., and Futrell, Jean H. Sat . "Surface-induced dissociation in a Fourier transform ion cyclotron resonance mass spectrometer: Instrument design and evaluation". United States.
@article{osti_15001088,
title = {Surface-induced dissociation in a Fourier transform ion cyclotron resonance mass spectrometer: Instrument design and evaluation},
author = {Laskin, Julia and Denisov, Eduard and Shukla, Anil K. and Barlow, Stephan E. and Futrell, Jean H.},
abstractNote = {A new Fourier Transform Ion Cyclotron Resonance mass spectrometer (FT-ICR MS) has been constructed in our laboratory. The instrument employs surface-induced dissociation (SID) as an activation method for obtaining structural information on biomolecules in the gas phase. Tandem SID mass spectra can be acquired using either a continuous or a pulsed mode of operation. Collision energy of precursor ion is controlled by a dc offset of the ICR cell. This approach eliminates defocusing of the ion beam by the ion transfer optics as a function of ion kinetic energy and constitutes a significant improvement over our previous experimental setup. Furthermore, it can be easily implemented on any FT-ICR mass spectrometer. Very high signal-to-noise ratios of 200-500 were obtained in single-scan SID mass spectra of model peptides with acquisition time less than 1.1 s. Reasonable SID signal was detected in single-scan spectra with total acquisition time of only 0.3 s. The high signal-to-noise ratio and the fast acquisition time point on a potential application of SID for high-throughput studies in FT-ICR MS.},
doi = {},
journal = {Analytical Chemistry},
number = ,
volume = 74,
place = {United States},
year = {Sat Dec 01 00:00:00 EST 2001},
month = {Sat Dec 01 00:00:00 EST 2001}
}