Evolutionary instability of CUG-Leu in the genetic code of budding yeasts

Krassowski, Tadeusz; Coughlan, Aisling Y.; Shen, Xing-Xing; Zhou, Xiaofan; Kominek, Jacek; Opulente, Dana A.; Riley, Robert; Grigoriev, Igor V.; Maheshwari, Nikunj; Shields, Denis C.; Kurtzman, Cletus P.; Hittinger, Chris Todd; Rokas, Antonis; Wolfe, Kenneth H.

doi:10.1038/s41467-018-04374-7

Title: Evolutionary instability of CUG-Leu in the genetic code of budding yeasts

Journal Article · Mon May 14 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-04374-7· OSTI ID:1500012

Krassowski, Tadeusz ^[1]; Coughlan, Aisling Y. ^[1];

^[2]; Zhou, Xiaofan ^[2];

^[3]; Opulente, Dana A. ^[3]; Riley, Robert ^[4];

^[5];

^[1]; Shields, Denis C. ^[1]; Kurtzman, Cletus P. ^[6];

^[3];

^[2];

^[1]

Univ. College Dublin (Ireland)
Vanderbilt Univ., Nashville, TN (United States)
Univ. of Wisconsin, Madison, WI (United States)
Amyris, Emeryville, CA (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Dept. of Agriculture, Peoria, IL (United States). Agricultural Research Station

The genetic code used in nuclear genes is almost universal, but here we report that it changed three times in parallel during the evolution of budding yeasts. All three changes were reassignments of the codon CUG, which is translated as serine (in 2 yeast clades), alanine (1 clade), or the 'universal' leucine (2 clades). The newly discovered Ser2 clade is in the final stages of a genetic code transition. Most species in this clade have genes for both a novel tRNASer(CAG) and an ancestral tRNALeu(CAG) to read CUG, but only tRNASer(CAG) is used in standard growth conditions. The coexistence of these alloacceptor tRNA genes indicates that the genetic code transition occurred via an ambiguous translation phase. We propose that the three parallel reassignments of CUG were not driven by natural selection in favor of their effects on the proteome, but by selection to eliminate the ancestral tRNALeu(CAG).

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: FC02-07ER64494; AC02-05CH11231

OSTI ID:: 1500012

Alternate ID(s):: OSTI ID: 1619100

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 42 works

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