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Title: Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical

Abstract

All cells obtain 2'-deoxyribonucleotides for DNA synthesis through the activity of a ribonucleotide reductase (RNR). The class I RNRs found in humans and pathogenic bacteria differ in ( i) use of Fe(II), Mn(II), or both for activation of the dinuclear-metallocofactor subunit, β; ( ii) reaction of the reduced dimetal center with dioxygen or superoxide for this activation; ( iii) requirement (or lack thereof) for a flavoprotein activase, NrdI, to provide the superoxide from O 2; and ( iv) use of either a stable tyrosyl radical or a high-valent dimetal cluster to initiate each turnover by oxidizing a cysteine residue in the α subunit to a radical (Cys•). The use of manganese by bacterial class I, subclass b-d RNRs, which contrasts with the exclusive use of iron by the eukaryotic Ia enzymes, appears to be a countermeasure of certain pathogens against iron deprivation imposed by their hosts. Here, we report a metal-free type of class I RNR (subclass e) from two human pathogens. The Cys• in its α subunit is generated by a stable, tyrosine-derived dihydroxyphenylalanine radical (DOPA•) in β. The three-electron oxidation producing DOPA• occurs in Escherichia coli only if the β is coexpressed with the NrdI activase encoded adjacentlymore » in the pathogen genome. We conclude the independence of this new RNR from transition metals, or the requirement for a single metal ion only transiently for activation, may afford the pathogens an even more potent countermeasure against transition metal-directed innate immunity.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2];  [2];  [3];  [3];  [1];  [3]; ORCiD logo [1];  [2];  [1];  [1];  [1];  [1]
  1. Pennsylvania State Univ., University Park, PA (United States)
  2. Univ. of Pennsylvania, Philadelphia, PA (United States)
  3. Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
Searle Scholars Program; National Institutes of Health (NIH); USDOE Office of Science (SC); National Cancer Institute (NCI); National Institute of General Medical Sciences (NIGMS); Michigan Economic Development Corporation and Michigan Technology Tri-Corridor
OSTI Identifier:
1499708
Grant/Contract Number:  
GM119707; GM116353; AC02-06CH11357; ACB-12002; AGM-12006; 1S10OD012289-01A1; 085P1000817
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America
Additional Journal Information:
Journal Volume: 115; Journal Issue: 40; Journal ID: ISSN 0027-8424
Publisher:
National Academy of Sciences
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA biosynthesis; semiquinone; DOPA

Citation Formats

Blaesi, Elizabeth J., Palowitch, Gavin M., Hu, Kai, Kim, Amelia J., Rose, Hannah R., Alapati, Rahul, Lougee, Marshall G., Kim, Hee Jong, Taguchi, Alexander T., Tan, Kong Ooi, Laremore, Tatiana N., Griffin, Robert G., Krebs, Carsten, Matthews, Megan L., Silakov, Alexey, Bollinger Jr., J. Martin, Allen, Benjamin D., and Boal, Amie K. Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical. United States: N. p., 2018. Web. doi:10.1073/pnas.1811993115.
Blaesi, Elizabeth J., Palowitch, Gavin M., Hu, Kai, Kim, Amelia J., Rose, Hannah R., Alapati, Rahul, Lougee, Marshall G., Kim, Hee Jong, Taguchi, Alexander T., Tan, Kong Ooi, Laremore, Tatiana N., Griffin, Robert G., Krebs, Carsten, Matthews, Megan L., Silakov, Alexey, Bollinger Jr., J. Martin, Allen, Benjamin D., & Boal, Amie K. Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical. United States. doi:10.1073/pnas.1811993115.
Blaesi, Elizabeth J., Palowitch, Gavin M., Hu, Kai, Kim, Amelia J., Rose, Hannah R., Alapati, Rahul, Lougee, Marshall G., Kim, Hee Jong, Taguchi, Alexander T., Tan, Kong Ooi, Laremore, Tatiana N., Griffin, Robert G., Krebs, Carsten, Matthews, Megan L., Silakov, Alexey, Bollinger Jr., J. Martin, Allen, Benjamin D., and Boal, Amie K. Mon . "Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical". United States. doi:10.1073/pnas.1811993115. https://www.osti.gov/servlets/purl/1499708.
@article{osti_1499708,
title = {Metal-free class Ie ribonucleotide reductase from pathogens initiates catalysis with a tyrosine-derived dihydroxyphenylalanine radical},
author = {Blaesi, Elizabeth J. and Palowitch, Gavin M. and Hu, Kai and Kim, Amelia J. and Rose, Hannah R. and Alapati, Rahul and Lougee, Marshall G. and Kim, Hee Jong and Taguchi, Alexander T. and Tan, Kong Ooi and Laremore, Tatiana N. and Griffin, Robert G. and Krebs, Carsten and Matthews, Megan L. and Silakov, Alexey and Bollinger Jr., J. Martin and Allen, Benjamin D. and Boal, Amie K.},
abstractNote = {All cells obtain 2'-deoxyribonucleotides for DNA synthesis through the activity of a ribonucleotide reductase (RNR). The class I RNRs found in humans and pathogenic bacteria differ in (i) use of Fe(II), Mn(II), or both for activation of the dinuclear-metallocofactor subunit, β; (ii) reaction of the reduced dimetal center with dioxygen or superoxide for this activation; (iii) requirement (or lack thereof) for a flavoprotein activase, NrdI, to provide the superoxide from O2; and (iv) use of either a stable tyrosyl radical or a high-valent dimetal cluster to initiate each turnover by oxidizing a cysteine residue in the α subunit to a radical (Cys•). The use of manganese by bacterial class I, subclass b-d RNRs, which contrasts with the exclusive use of iron by the eukaryotic Ia enzymes, appears to be a countermeasure of certain pathogens against iron deprivation imposed by their hosts. Here, we report a metal-free type of class I RNR (subclass e) from two human pathogens. The Cys• in its α subunit is generated by a stable, tyrosine-derived dihydroxyphenylalanine radical (DOPA•) in β. The three-electron oxidation producing DOPA• occurs in Escherichia coli only if the β is coexpressed with the NrdI activase encoded adjacently in the pathogen genome. We conclude the independence of this new RNR from transition metals, or the requirement for a single metal ion only transiently for activation, may afford the pathogens an even more potent countermeasure against transition metal-directed innate immunity.},
doi = {10.1073/pnas.1811993115},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
issn = {0027-8424},
number = 40,
volume = 115,
place = {United States},
year = {2018},
month = {9}
}

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    Works referencing / citing this record:

    MaveDB: an open-source platform to distribute and interpret data from multiplexed assays of variant effect
    journal, November 2019


    Class Id ribonucleotide reductase utilizes a Mn2(IV,III) cofactor and undergoes large conformational changes on metal loading
    text, January 2019

    • Rozman Grinberg, Inna; Berglund, Sigrid; Hasan, Mahmudul
    • Deutsches Elektronen-Synchrotron, DESY, Hamburg
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