Structural basis for the flexible recognition of α-glucan substrates by Bacteroides thetaiotaomicron SusG: Structure of B. thetaiotaomicron SusG with a mixed linkage α-glucan product

doi:https://doi.org/10.1002/pro.3410

Title: Structural basis for the flexible recognition of α-glucan substrates by Bacteroides thetaiotaomicron SusG: Structure of B. thetaiotaomicron SusG with a mixed linkage α-glucan product

Authors:

Arnal, Gregory ^[1]; Cockburn, Darrell W. ^[2]; Brumer, Harry ^[3];

^[2]

Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver British Columbia V6T 1Z4 Canada
Department of Microbiology and Immunology, University of Michigan Medical School, 1150 W Medical Center Drive, Ann Arbor Michigan
Michael Smith Laboratories, University of British Columbia, 2185 East Mall, Vancouver British Columbia V6T 1Z4 Canada; Department of Chemistry, University of British Columbia, 2036 Main Mall, Vancouver British Columbia V6T 1Z1 Canada; Department of Biochemistry and Molecular Biology, University of British Columbia, 2350 Health Sciences Mall, Vancouver British Columbia V6T 1Z3 Canada

Publication Date:: 2018-04-17

Research Org.:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Org.:: UNIVERSITYNIH

OSTI Identifier:: 1498430

Resource Type:: Journal Article

Journal Name:: Protein Science

Additional Journal Information:: Journal Volume: 27; Journal Issue: 6; Journal ID: ISSN 0961-8368

Publisher:: The Protein Society

Country of Publication:: United States

Language:: ENGLISH

Citation Formats


                    Arnal, Gregory, Cockburn, Darrell W., Brumer, Harry, and Koropatkin, Nicole M. Structural basis for the flexible recognition of α-glucan substrates by Bacteroides thetaiotaomicron SusG: Structure of B. thetaiotaomicron SusG with a mixed linkage α-glucan product.  United States: N. p., 2018. 
        Web.  doi:10.1002/pro.3410.

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                    Arnal, Gregory, Cockburn, Darrell W., Brumer, Harry, & Koropatkin, Nicole M. Structural basis for the flexible recognition of α-glucan substrates by Bacteroides thetaiotaomicron SusG: Structure of B. thetaiotaomicron SusG with a mixed linkage α-glucan product.  United States.  https://doi.org/10.1002/pro.3410

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                    Arnal, Gregory, Cockburn, Darrell W., Brumer, Harry, and Koropatkin, Nicole M. Tue .  
        "Structural basis for the flexible recognition of α-glucan substrates by Bacteroides thetaiotaomicron SusG: Structure of B. thetaiotaomicron SusG with a mixed linkage α-glucan product".  United States.  https://doi.org/10.1002/pro.3410.

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@article{osti_1498430,

  title        = {Structural basis for the flexible recognition of α-glucan substrates by Bacteroides thetaiotaomicron SusG: Structure of B. thetaiotaomicron SusG with a mixed linkage α-glucan product},

  author       = {Arnal, Gregory and Cockburn, Darrell W. and Brumer, Harry and Koropatkin, Nicole M.},

  abstractNote = {},

  doi          = {10.1002/pro.3410},

  url          = {https://www.osti.gov/biblio/1498430},
  journal      = {Protein Science},

  issn         = {0961-8368},

  number       = 6,

  volume       = 27,

  place        = {United States},

  year         = {2018},

  month        = {4}

}

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Title: Structural basis for the flexible recognition of α-glucan substrates by Bacteroides thetaiotaomicron SusG: Structure of B. thetaiotaomicron SusG with a mixed linkage α-glucan product

Citation Formats

Detailed Kinetic Analysis and Identification of the Nucleophile in α-l-Arabinofuranosidase from Geobacillus stearothermophilus T-6, a Family 51 Glycoside Hydrolase journal, September 2002

Chemical Synthesis of a Dual Branched Malto-Decaose: A Potential Substrate for α-Amylases journal, June 2005

Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly-extreme condition journal, April 2006

Slow glucose release property of enzyme-synthesized highly branched maltodextrins differs among starch sources journal, July 2014

Crystal Structure of Thermotoga maritima 4-α-Glucanotransferase and its Acarbose Complex: Implications for Substrate Specificity and Catalysis journal, August 2002

Cyclomaltodextrinase, Neopullulanase, and Maltogenic Amylase Are Nearly Indistinguishable from Each Other journal, March 2002

Regulation of glycogen metabolism in yeast and bacteria journal, November 2010

Structures of Thermoactinomyces vulgaris R-47 α-Amylase II Complexed with Substrate Analogues journal, January 2001

Properties and applications of starch-converting enzymes of the α-amylase family journal, March 2002

The crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) complexed with transglycosylated product journal, June 2004

Privateer: software for the conformational validation of carbohydrate structures journal, November 2015

Mutagenesis of Glycosidases journal, June 1999

Amylase action pattern on starch polymers journal, January 2008

Enzyme-Synthesized Highly Branched Maltodextrins Have Slow Glucose Generation at the Mucosal α-Glucosidase Level and Are Slowly Digestible In Vivo journal, April 2013

Nomenclature for sugar-binding subsites in glycosyl hydrolases journal, January 1997

Corrigendum: Glycosynthases in Biocatalysis journal, November 2011

Conversion of Neopullulanase- -Amylase from Thermoactinomyces vulgaris R-47 into an Amylopullulanase-Type Enzyme journal, February 1998

Tracing the evolution of the α-amylase subfamily GH13_36 covering the amylolytic enzymes intermediate between oligo-1,6-glucosidases and neopullulanases journal, February 2013

Preferential and rapid degradation of raw rice starch by an α-amylase of glycoside hydrolase subfamily GH13_37 journal, May 2012

SusG: A Unique Cell-Membrane-Associated α-Amylase from a Prominent Human Gut Symbiont Targets Complex Starch Molecules journal, February 2010

Structural biology of starch-degrading enzymes and their regulation journal, October 2016

Dividing the large glycoside hydrolase family 13 into subfamilies: towards improved functional annotations of -amylase-related proteins journal, September 2006

Modulation of activity and substrate binding modes by mutation of single and double subsites +1/+2 and −5/−6 of barley α-amylase 1: Barley α-amylase subsite mutants journal, December 2001

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases journal, June 2013

Detailed Kinetic Analysis and Identification of the Nucleophile in α-l-Arabinofuranosidase from Geobacillus stearothermophilus T-6, a Family 51 Glycoside Hydrolase
journal, September 2002

Chemical Synthesis of a Dual Branched Malto-Decaose: A Potential Substrate for α-Amylases
journal, June 2005

Crystal structure of AmyA lacks acidic surface and provide insights into protein stability at poly-extreme condition
journal, April 2006

Slow glucose release property of enzyme-synthesized highly branched maltodextrins differs among starch sources
journal, July 2014

Crystal Structure of Thermotoga maritima 4-α-Glucanotransferase and its Acarbose Complex: Implications for Substrate Specificity and Catalysis
journal, August 2002

Cyclomaltodextrinase, Neopullulanase, and Maltogenic Amylase Are Nearly Indistinguishable from Each Other
journal, March 2002

Regulation of glycogen metabolism in yeast and bacteria
journal, November 2010

Structures of Thermoactinomyces vulgaris R-47 α-Amylase II Complexed with Substrate Analogues
journal, January 2001

Properties and applications of starch-converting enzymes of the α-amylase family
journal, March 2002

The crystal structure of Thermoactinomyces vulgaris R-47 alpha-amylase II (TVA II) complexed with transglycosylated product
journal, June 2004

Privateer: software for the conformational validation of carbohydrate structures
journal, November 2015

Mutagenesis of Glycosidases
journal, June 1999

Amylase action pattern on starch polymers
journal, January 2008

Enzyme-Synthesized Highly Branched Maltodextrins Have Slow Glucose Generation at the Mucosal α-Glucosidase Level and Are Slowly Digestible In Vivo
journal, April 2013

Nomenclature for sugar-binding subsites in glycosyl hydrolases
journal, January 1997

Corrigendum: Glycosynthases in Biocatalysis
journal, November 2011

Conversion of Neopullulanase- -Amylase from Thermoactinomyces vulgaris R-47 into an Amylopullulanase-Type Enzyme
journal, February 1998

Tracing the evolution of the α-amylase subfamily GH13_36 covering the amylolytic enzymes intermediate between oligo-1,6-glucosidases and neopullulanases
journal, February 2013

Preferential and rapid degradation of raw rice starch by an α-amylase of glycoside hydrolase subfamily GH13_37
journal, May 2012

SusG: A Unique Cell-Membrane-Associated α-Amylase from a Prominent Human Gut Symbiont Targets Complex Starch Molecules
journal, February 2010

Structural biology of starch-degrading enzymes and their regulation
journal, October 2016

Dividing the large glycoside hydrolase family 13 into subfamilies: towards improved functional annotations of -amylase-related proteins
journal, September 2006

Modulation of activity and substrate binding modes by mutation of single and double subsites +1/+2 and −5/−6 of barley α-amylase 1: Barley α-amylase subsite mutants
journal, December 2001

α-Amylase: an enzyme specificity found in various families of glycoside hydrolases
journal, June 2013