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Title: Lesion orientation of O 4 -alkylthymidine influences replication by human DNA polymerase η

Abstract

Conformation of the α-carbon of O 4-alkylthymidine was shown to exert an influence on human DNA polymerase η (hPol η) bypass. Crystal structures of hPol η·DNA·dNTP ternary complexes reveal a unique conformation adopted by O 4-methylthymidine, where the nucleobase resides nestled at the active site ceiling where hydrogen-bonding with the incoming nucleotide is prevented.

Authors:
 [1];  [2];  [2];  [2];  [2];  [1]
  1. Department of Chemistry and Biochemistry; Concordia University; Montréal; Canada
  2. Department of Biochemistry; Vanderbilt Institute of Chemical Biology; Center for Structural Biology; School of Medicine; Vanderbilt University
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1498411
Resource Type:
Journal Article
Journal Name:
Chemical Science
Additional Journal Information:
Journal Volume: 7; Journal Issue: 8; Journal ID: ISSN 2041-6520
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
ENGLISH

Citation Formats

O'Flaherty, D. K., Patra, A., Su, Y., Guengerich, F. P., Egli, M., and Wilds, C. J. Lesion orientation of O 4 -alkylthymidine influences replication by human DNA polymerase η. United States: N. p., 2016. Web. doi:10.1039/C6SC00666C.
O'Flaherty, D. K., Patra, A., Su, Y., Guengerich, F. P., Egli, M., & Wilds, C. J. Lesion orientation of O 4 -alkylthymidine influences replication by human DNA polymerase η. United States. doi:10.1039/C6SC00666C.
O'Flaherty, D. K., Patra, A., Su, Y., Guengerich, F. P., Egli, M., and Wilds, C. J. Fri . "Lesion orientation of O 4 -alkylthymidine influences replication by human DNA polymerase η". United States. doi:10.1039/C6SC00666C.
@article{osti_1498411,
title = {Lesion orientation of O 4 -alkylthymidine influences replication by human DNA polymerase η},
author = {O'Flaherty, D. K. and Patra, A. and Su, Y. and Guengerich, F. P. and Egli, M. and Wilds, C. J.},
abstractNote = {Conformation of the α-carbon of O4-alkylthymidine was shown to exert an influence on human DNA polymerase η (hPol η) bypass. Crystal structures of hPol η·DNA·dNTP ternary complexes reveal a unique conformation adopted by O4-methylthymidine, where the nucleobase resides nestled at the active site ceiling where hydrogen-bonding with the incoming nucleotide is prevented.},
doi = {10.1039/C6SC00666C},
journal = {Chemical Science},
issn = {2041-6520},
number = 8,
volume = 7,
place = {United States},
year = {2016},
month = {1}
}

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