skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin

; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH)
OSTI Identifier:
Resource Type:
Journal Article
Journal Name:
Nature (London)
Additional Journal Information:
Journal Volume: 566; Journal Issue: 7742; Journal ID: ISSN 0028-0836
Nature Publishing Group
Country of Publication:
United States

Citation Formats

Ng, Tai L., Rohac, Roman, Mitchell, Andrew J., Boal, Amie K., and Balskus, Emily P. An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin. United States: N. p., 2019. Web. doi:10.1038/s41586-019-0894-z.
Ng, Tai L., Rohac, Roman, Mitchell, Andrew J., Boal, Amie K., & Balskus, Emily P. An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin. United States. doi:10.1038/s41586-019-0894-z.
Ng, Tai L., Rohac, Roman, Mitchell, Andrew J., Boal, Amie K., and Balskus, Emily P. Fri . "An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin". United States. doi:10.1038/s41586-019-0894-z.
title = {An N-nitrosating metalloenzyme constructs the pharmacophore of streptozotocin},
author = {Ng, Tai L. and Rohac, Roman and Mitchell, Andrew J. and Boal, Amie K. and Balskus, Emily P.},
abstractNote = {},
doi = {10.1038/s41586-019-0894-z},
journal = {Nature (London)},
issn = {0028-0836},
number = 7742,
volume = 566,
place = {United States},
year = {2019},
month = {2}

Works referenced in this record:

The nitrate–nitrite–nitric oxide pathway in physiology and therapeutics
journal, February 2008

  • Lundberg, Jon O.; Weitzberg, Eddie; Gladwin, Mark T.
  • Nature Reviews Drug Discovery, Vol. 7, Issue 2
  • DOI: 10.1038/nrd2466

Nitrate, bacteria and human health
journal, July 2004

  • Lundberg, Jon O.; Weitzberg, Eddie; Cole, Jeff A.
  • Nature Reviews Microbiology, Vol. 2, Issue 7
  • DOI: 10.1038/nrmicro929

The nitrosoureas: carmustine (BCNU) and lomustine (CCNU)
journal, December 1982

Chemical Dissection of the Link between Streptozotocin, O-GlcNAc, and Pancreatic Cell Death
journal, August 2008

Genotoxicity of Streptozotocin
journal, December 2002

  • Bolzán, Alejandro D.; Bianchi, Martha S.
  • Mutation Research/Reviews in Mutation Research, Vol. 512, Issue 2-3
  • DOI: 10.1016/S1383-5742(02)00044-3

Convergence of biological nitration and nitrosation via symmetrical nitrous anhydride
journal, May 2015

  • Vitturi, Dario A.; Minarrieta, Lucia; Salvatore, Sonia R.
  • Nature Chemical Biology, Vol. 11, Issue 7
  • DOI: 10.1038/nchembio.1814

Balancing repair and tolerance of DNA damage caused by alkylating agents
journal, January 2012

  • Fu, Dragony; Calvo, Jennifer A.; Samson, Leona D.
  • Nature Reviews Cancer, Vol. 12, Issue 2
  • DOI: 10.1038/nrc3185

Diiron monooxygenases in natural product biosynthesis
journal, January 2018

  • Komor, Anna J.; Jasniewski, Andrew J.; Que, Lawrence
  • Natural Product Reports, Vol. 35, Issue 7
  • DOI: 10.1039/C7NP00061H

Reconstitution and Characterization of Aminopyrrolnitrin Oxygenase, a Rieske N -Oxygenase That Catalyzes Unusual Arylamine Oxidation
journal, September 2005

  • Lee, Jungkul; Simurdiak, Michael; Zhao, Huimin
  • Journal of Biological Chemistry, Vol. 280, Issue 44
  • DOI: 10.1074/jbc.M505334200

Structural Determinants for the Strict Monomethylation Activity by Trypanosoma brucei Protein Arginine Methyltransferase 7
journal, May 2014

Microbial biosynthesis of medium-chain 1-alkenes by a nonheme iron oxidase
journal, December 2014

  • Rui, Zhe; Li, Xin; Zhu, Xuejun
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 51
  • DOI: 10.1073/pnas.1419701112

Bacterial Nitric Oxide Synthases
journal, June 2010

Polder maps: improving OMIT maps by excluding bulk solvent
journal, February 2017

  • Liebschner, Dorothee; Afonine, Pavel V.; Moriarty, Nigel W.
  • Acta Crystallographica Section D Structural Biology, Vol. 73, Issue 2, p. 148-157
  • DOI: 10.1107/S2059798316018210

Heme Enzyme Structure and Function
journal, January 2014

Structure of the Chlamydia Protein CADD Reveals a Redox Enzyme That Modulates Host Cell Apoptosis
journal, April 2004

  • Schwarzenbacher, Robert; Stenner-Liewen, Frank; Liewen, Heike
  • Journal of Biological Chemistry, Vol. 279, Issue 28
  • DOI: 10.1074/jbc.M401268200

Dioxygen Activation and Methane Hydroxylation by Soluble Methane Monooxygenase: A Tale of Two Irons and Three Proteins
journal, August 2001

Dioxygen activation by nonheme iron enzymes with the 2-His-1-carboxylate facial triad that generate high-valent oxoiron oxidants
journal, January 2017

  • Kal, Subhasree; Que, Lawrence
  • JBIC Journal of Biological Inorganic Chemistry, Vol. 22, Issue 2-3
  • DOI: 10.1007/s00775-016-1431-2

Nitrosomonas europaea cytochrome P460 is a direct link between nitrification and nitrous oxide emission
journal, November 2016

  • Caranto, Jonathan D.; Vilbert, Avery C.; Lancaster, Kyle M.
  • Proceedings of the National Academy of Sciences, Vol. 113, Issue 51
  • DOI: 10.1073/pnas.1611051113

Gramibactin is a bacterial siderophore with a diazeniumdiolate ligand system
journal, July 2018

Prokka: rapid prokaryotic genome annotation
journal, March 2014

Mauve: Multiple Alignment of Conserved Genomic Sequence With Rearrangements
journal, June 2004

The HHpred interactive server for protein homology detection and structure prediction
journal, July 2005

  • Soding, J.; Biegert, A.; Lupas, A. N.
  • Nucleic Acids Research, Vol. 33, Issue Web Server
  • DOI: 10.1093/nar/gki408

Clustal W and Clustal X version 2.0
journal, September 2007

Comparative Protein Structure Modeling Using Modeller
journal, September 2006

PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin
journal, January 2003

  • Gust, B.; Challis, G. L.; Fowler, K.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 4
  • DOI: 10.1073/pnas.0337542100

Direct measurement of nitric oxide generation from nitric oxide synthase
journal, November 1997

  • Xia, Y.; Zweier, J. L.
  • Proceedings of the National Academy of Sciences, Vol. 94, Issue 23
  • DOI: 10.1073/pnas.94.23.12705

Rapid and efficient site-specific mutagenesis without phenotypic selection.
journal, January 1985

  • Kunkel, T. A.
  • Proceedings of the National Academy of Sciences, Vol. 82, Issue 2, p. 488-492
  • DOI: 10.1073/pnas.82.2.488

MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms
journal, May 2018

  • Kumar, Sudhir; Stecher, Glen; Li, Michael
  • Molecular Biology and Evolution, Vol. 35, Issue 6
  • DOI: 10.1093/molbev/msy096

IMG: the integrated microbial genomes database and comparative analysis system
journal, December 2011

  • Markowitz, V. M.; Chen, I. -M. A.; Palaniappan, K.
  • Nucleic Acids Research, Vol. 40, Issue D1
  • DOI: 10.1093/nar/gkr1044

Formation of the Pyridazine Natural Product Azamerone by Biosynthetic Rearrangement of an Aryl Diazoketone
journal, January 2009

  • Winter, Jaclyn M.; Jansma, Ariane L.; Handel, Tracy M.
  • Angewandte Chemie International Edition, Vol. 48, Issue 4
  • DOI: 10.1002/anie.200805140

Glutamic acid is a carrier for hydrazine during the biosyntheses of fosfazinomycin and kinamycin
journal, September 2018

Spectrophotometric Determination of Streptozotocin.
journal, June 1964

Crystal Structure of Dimeric Heme Oxygenase-2 from Synechocystis sp. PCC 6803 in Complex with Heme ,
journal, March 2005

  • Sugishima, Masakazu; Hagiwara, Yoshinori; Zhang, Xuhong
  • Biochemistry, Vol. 44, Issue 11
  • DOI: 10.1021/bi0480483

Promiscuous and Adaptable Enzymes Fill “Holes” in the Tetrahydrofolate Pathway in Chlamydia Species
journal, July 2014

  • Adams, Nancy E.; Thiaville, Jennifer J.; Proestos, James
  • mBio, Vol. 5, Issue 4
  • DOI: 10.1128/mBio.01378-14

PROMALS web server for accurate multiple protein sequence alignments
journal, May 2007

  • Pei, J.; Kim, B. -H.; Tang, M.
  • Nucleic Acids Research, Vol. 35, Issue Web Server
  • DOI: 10.1093/nar/gkm227

Quinone biogenesis: Structure and mechanism of PqqC, the final catalyst in the production of pyrroloquinoline quinone
journal, May 2004

  • Magnusson, O. Th.; Toyama, H.; Saeki, M.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 21
  • DOI: 10.1073/pnas.0402640101