Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations

Nagy, Gabe; Veličković, Dusan; Chu, Rosalie K.; Carrell, Alyssa A.; Weston, David J.; Ibrahim, Yehia M.; Anderton, Christopher R.; Smith, Richard D.

doi:10.1039/C8CC07482H

Title: Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations

Journal Article · Tue Jan 01 00:00:00 EST 2019 · ChemComm

DOI:https://doi.org/10.1039/C8CC07482H· OSTI ID:1496826

Nagy, Gabe ^[1]; Veličković, Dusan ^[1]; Chu, Rosalie K. ^[2]; Carrell, Alyssa A. ^[3]; Weston, David J. ^[3];

^[1];

^[2];

^[1]

Biological Sciences Division, Pacific Northwest National Laboratory; Richland; USA
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory; Richland; USA
Biosciences Division; Oak Ridge National Laboratory; Oak Ridge; USA

We demonstrate the coupling of liquid extraction surface analysis (LESA) to structures for lossless ion manipulations in conjunction with serpentine ultralong path with extending routing (SLIM SUPER) ion mobility-mass spectrometry (IM-MS) for the unambiguous annotation of important isomeric glycoforms in carbon-fixing communities. A major bottleneck of current mass spectrometry imaging (MSI) methods is their inability to accurately and confidently delineate amongst isomers within a single probing area. Our methodology provides gains in both sensitivity and IM resolution, while simultaneously gaining insight on the spatial distribution of these biological isomers in a mixed organism composed of moss, cyanobacteria and fungus. We envision this methodology will be broadly applicable to other mass spectrometry imaging modalities and invaluable in multiplexed, untargeted, analyses of a single probing location.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1496826

Report Number(s):: PNNL-SA-138045; CHCOFS

Journal Information:: ChemComm, Vol. 55, Issue 3; ISSN 1359-7345

Publisher:: Royal Society of Chemistry

Country of Publication:: United States

Language:: English

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