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Title: Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations

Abstract

We demonstrate the coupling of liquid extraction surface analysis (LESA) to structures for lossless ion manipulations in conjunction with serpentine ultralong path with extending routing (SLIM SUPER) ion mobility-mass spectrometry (IM-MS) for the unambiguous annotation of important isomeric glycoforms in carbon-fixing communities. A major bottleneck of current mass spectrometry imaging (MSI) methods is their inability to accurately and confidently delineate amongst isomers within a single probing area. Our methodology provides gains in both sensitivity and IM resolution, while simultaneously gaining insight on the spatial distribution of these biological isomers in a mixed organism composed of moss, cyanobacteria and fungus. We envision this methodology will be broadly applicable to other mass spectrometry imaging modalities and invaluable in multiplexed, untargeted, analyses of a single probing location.

Authors:
 [1];  [1];  [2];  [3];  [3]; ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1]
  1. Biological Sciences Division, Pacific Northwest National Laboratory; Richland; USA
  2. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory; Richland; USA
  3. Biosciences Division; Oak Ridge National Laboratory; Oak Ridge; USA
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1496826
Report Number(s):
PNNL-SA-138045
Journal ID: ISSN 1359-7345; CHCOFS
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
ChemComm
Additional Journal Information:
Journal Volume: 55; Journal Issue: 3; Journal ID: ISSN 1359-7345
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English

Citation Formats

Nagy, Gabe, Veličković, Dusan, Chu, Rosalie K., Carrell, Alyssa A., Weston, David J., Ibrahim, Yehia M., Anderton, Christopher R., and Smith, Richard D. Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations. United States: N. p., 2019. Web. doi:10.1039/C8CC07482H.
Nagy, Gabe, Veličković, Dusan, Chu, Rosalie K., Carrell, Alyssa A., Weston, David J., Ibrahim, Yehia M., Anderton, Christopher R., & Smith, Richard D. Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations. United States. doi:10.1039/C8CC07482H.
Nagy, Gabe, Veličković, Dusan, Chu, Rosalie K., Carrell, Alyssa A., Weston, David J., Ibrahim, Yehia M., Anderton, Christopher R., and Smith, Richard D. Tue . "Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations". United States. doi:10.1039/C8CC07482H.
@article{osti_1496826,
title = {Towards Resolving the Spatial Metabolome with Unambiguous Molecular Annotations in Complex Biological Systems by Coupling Mass Spectrometry Imaging with Structures for Lossless Ion Manipulations},
author = {Nagy, Gabe and Veličković, Dusan and Chu, Rosalie K. and Carrell, Alyssa A. and Weston, David J. and Ibrahim, Yehia M. and Anderton, Christopher R. and Smith, Richard D.},
abstractNote = {We demonstrate the coupling of liquid extraction surface analysis (LESA) to structures for lossless ion manipulations in conjunction with serpentine ultralong path with extending routing (SLIM SUPER) ion mobility-mass spectrometry (IM-MS) for the unambiguous annotation of important isomeric glycoforms in carbon-fixing communities. A major bottleneck of current mass spectrometry imaging (MSI) methods is their inability to accurately and confidently delineate amongst isomers within a single probing area. Our methodology provides gains in both sensitivity and IM resolution, while simultaneously gaining insight on the spatial distribution of these biological isomers in a mixed organism composed of moss, cyanobacteria and fungus. We envision this methodology will be broadly applicable to other mass spectrometry imaging modalities and invaluable in multiplexed, untargeted, analyses of a single probing location.},
doi = {10.1039/C8CC07482H},
journal = {ChemComm},
issn = {1359-7345},
number = 3,
volume = 55,
place = {United States},
year = {2019},
month = {1}
}