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Title: Atmo-ecometabolomics: A novel atmospheric particle chemical characterization methodology for ecological research

Abstract

Aerosol particles play important roles in processes controlling the composition of the atmosphere and function of ecosystems. A better understanding of the composition of aerosol particles is beginning to be recognized as critical for ecological research to further comprehend the link between aerosols and ecosystems. While chemical characterization of aerosols has been practiced in the atmospheric science community, detailed methodology tailored to the needs of ecological research does not exist yet. In this study, we describe an efficient methodology (atmo-ecometabolomics), in step-by-step details, from the sampling to the data analyses, to characterize the chemical composition of aerosol particles, namely atmo-metabolome. This method employs mass spectrometry platforms such as liquid and gas chromatography mass spectrometries (MS) and Fourier transform ion cyclotron resonance MS (FT-ICR-MS). For methodology evaluation, we analyzed aerosol particles collected during two different seasons (spring and summer) in a low-biologicalactivity ecosystem. Additionally, to further validate our methodology, we analyzed aerosol particles collected in a more biologically active ecosystem during the pollination peaks of three different representative tree species. Our statistical results showed that our sampling and extraction methods are suitable for characterizing the atmo-ecometabolomes in these two distinct ecosystems with any of the analytical platforms. Datasets obtained from eachmore » mass spectrometry instrument showed overall significant differences of the atmo-ecometabolomes between spring and summer as well as between the three pollination peak periods. Furthermore, we have identified several metabolites that can be attributed to pollen and other plant-related aerosol particles.We additionally provide a basic guide of the potential use ecometabolomic techniques on different mass spectrometry platforms to accurately analyze the atmoecometabolomes for ecological studies. Our method represents an advanced novel approach for future studies in the impact of aerosol particle chemical compositions on ecosystem structure and function and biogeochemistry.« less

Authors:
ORCiD logo; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1495028
Report Number(s):
PNNL-SA-118888
Journal ID: ISSN 0167-6369
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Environmental Monitoring and Assessment
Additional Journal Information:
Journal Volume: 191; Journal Issue: 2; Journal ID: ISSN 0167-6369
Publisher:
Springer
Country of Publication:
United States
Language:
English

Citation Formats

Rivas-Ubach, Albert, Liu, Yina, Steiner, Allison L., Sardans, Jordi, Tfaily, Malak M., Kulkarni, Gourihar, Kim, Young-Mo, Bourrianne, Eric, Paša-Tolić, Ljiljana, Peñuelas, Josep, and Guenther, Alex. Atmo-ecometabolomics: A novel atmospheric particle chemical characterization methodology for ecological research. United States: N. p., 2019. Web. doi:10.1007/s10661-019-7205-x.
Rivas-Ubach, Albert, Liu, Yina, Steiner, Allison L., Sardans, Jordi, Tfaily, Malak M., Kulkarni, Gourihar, Kim, Young-Mo, Bourrianne, Eric, Paša-Tolić, Ljiljana, Peñuelas, Josep, & Guenther, Alex. Atmo-ecometabolomics: A novel atmospheric particle chemical characterization methodology for ecological research. United States. doi:10.1007/s10661-019-7205-x.
Rivas-Ubach, Albert, Liu, Yina, Steiner, Allison L., Sardans, Jordi, Tfaily, Malak M., Kulkarni, Gourihar, Kim, Young-Mo, Bourrianne, Eric, Paša-Tolić, Ljiljana, Peñuelas, Josep, and Guenther, Alex. Wed . "Atmo-ecometabolomics: A novel atmospheric particle chemical characterization methodology for ecological research". United States. doi:10.1007/s10661-019-7205-x.
@article{osti_1495028,
title = {Atmo-ecometabolomics: A novel atmospheric particle chemical characterization methodology for ecological research},
author = {Rivas-Ubach, Albert and Liu, Yina and Steiner, Allison L. and Sardans, Jordi and Tfaily, Malak M. and Kulkarni, Gourihar and Kim, Young-Mo and Bourrianne, Eric and Paša-Tolić, Ljiljana and Peñuelas, Josep and Guenther, Alex},
abstractNote = {Aerosol particles play important roles in processes controlling the composition of the atmosphere and function of ecosystems. A better understanding of the composition of aerosol particles is beginning to be recognized as critical for ecological research to further comprehend the link between aerosols and ecosystems. While chemical characterization of aerosols has been practiced in the atmospheric science community, detailed methodology tailored to the needs of ecological research does not exist yet. In this study, we describe an efficient methodology (atmo-ecometabolomics), in step-by-step details, from the sampling to the data analyses, to characterize the chemical composition of aerosol particles, namely atmo-metabolome. This method employs mass spectrometry platforms such as liquid and gas chromatography mass spectrometries (MS) and Fourier transform ion cyclotron resonance MS (FT-ICR-MS). For methodology evaluation, we analyzed aerosol particles collected during two different seasons (spring and summer) in a low-biologicalactivity ecosystem. Additionally, to further validate our methodology, we analyzed aerosol particles collected in a more biologically active ecosystem during the pollination peaks of three different representative tree species. Our statistical results showed that our sampling and extraction methods are suitable for characterizing the atmo-ecometabolomes in these two distinct ecosystems with any of the analytical platforms. Datasets obtained from each mass spectrometry instrument showed overall significant differences of the atmo-ecometabolomes between spring and summer as well as between the three pollination peak periods. Furthermore, we have identified several metabolites that can be attributed to pollen and other plant-related aerosol particles.We additionally provide a basic guide of the potential use ecometabolomic techniques on different mass spectrometry platforms to accurately analyze the atmoecometabolomes for ecological studies. Our method represents an advanced novel approach for future studies in the impact of aerosol particle chemical compositions on ecosystem structure and function and biogeochemistry.},
doi = {10.1007/s10661-019-7205-x},
journal = {Environmental Monitoring and Assessment},
issn = {0167-6369},
number = 2,
volume = 191,
place = {United States},
year = {2019},
month = {1}
}