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Title: Production of biofuels and chemicals from xylose using native and engineered yeast strains

Abstract

Numerous metabolic engineering strategies have allowed yeasts to efficiently assimilate xylose, the second most abundant sugar component of lignocellulosic biomass. During the investigation of xylose utilization by yeasts, a global rewiring of metabolic networks upon xylose cultivation has been captured, as opposed to a pattern of glucose repression. A clear understanding of the xylose-induced metabolic reprogramming in yeast would shed light on the optimization of yeast-based bioprocesses to produce biofuels and chemicals using xylose. In this review, we delved into the characteristics of yeast xylose metabolism, and potential benefits of using xylose as a carbon source to produce various biochemicals with examples. Transcriptomic and metabolomic patterns of xylose-grown yeast cells were distinct from those on glucose—a conventional sugar of industrial biotechnology—and the gap might lead to opportunities to produce biochemicals efficiently. Indeed, limited glycolytic metabolic fluxes during xylose utilization could result in enhanced production of metabolites whose biosynthetic pathways compete for precursors with ethanol fermentation. Also, alleviation of glucose repression on cytosolic acetyl coenzyme A (acetyl-CoA) synthesis, and respiratory energy metabolism during xylose utilization enhanced production of acetyl-CoA derivatives. Consideration of singular properties of xylose metabolism, such as redox cofactor imbalance between xylose reductase and xylitol dehydrogenase, is necessary tomore » maximize these positive xylose effects. This review argues the importance and benefits of xylose utilization as not only a way of expanding a substrate range, but also an effective environmental perturbation for the efficient production of advanced biofuels and chemicals in yeasts.« less

Authors:
 [1];  [2];  [3];  [1]; ORCiD logo [2]
  1. Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology
  2. Seoul National Univ. (Korea, Republic of). Dept. of Agricultural Biotechnology and Center for Food and Bioconvergence
  3. Univ. of Illinois, Urbana-Champaign, IL (United States). Carl R. Woese Inst. for Genomic Biology; Korea Univ., Seoul (South Korea). Dept. of Biotechnology, Graduate School
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI); Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Korea Institute for Industrial Economics and Trade (KIET)
OSTI Identifier:
1495021
Alternate Identifier(s):
OSTI ID: 1487444
Grant/Contract Number:  
SC0018420
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology Advances
Additional Journal Information:
Journal Volume: 37; Journal Issue: 2; Journal ID: ISSN 0734-9750
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS

Citation Formats

Kwak, Suryang, Jo, Jung Hyun, Yun, Eun Ju, Jin, Yong-Su, and Seo, Jin-Ho. Production of biofuels and chemicals from xylose using native and engineered yeast strains. United States: N. p., 2019. Web. doi:10.1016/j.biotechadv.2018.12.003.
Kwak, Suryang, Jo, Jung Hyun, Yun, Eun Ju, Jin, Yong-Su, & Seo, Jin-Ho. Production of biofuels and chemicals from xylose using native and engineered yeast strains. United States. doi:10.1016/j.biotechadv.2018.12.003.
Kwak, Suryang, Jo, Jung Hyun, Yun, Eun Ju, Jin, Yong-Su, and Seo, Jin-Ho. Fri . "Production of biofuels and chemicals from xylose using native and engineered yeast strains". United States. doi:10.1016/j.biotechadv.2018.12.003. https://www.osti.gov/servlets/purl/1495021.
@article{osti_1495021,
title = {Production of biofuels and chemicals from xylose using native and engineered yeast strains},
author = {Kwak, Suryang and Jo, Jung Hyun and Yun, Eun Ju and Jin, Yong-Su and Seo, Jin-Ho},
abstractNote = {Numerous metabolic engineering strategies have allowed yeasts to efficiently assimilate xylose, the second most abundant sugar component of lignocellulosic biomass. During the investigation of xylose utilization by yeasts, a global rewiring of metabolic networks upon xylose cultivation has been captured, as opposed to a pattern of glucose repression. A clear understanding of the xylose-induced metabolic reprogramming in yeast would shed light on the optimization of yeast-based bioprocesses to produce biofuels and chemicals using xylose. In this review, we delved into the characteristics of yeast xylose metabolism, and potential benefits of using xylose as a carbon source to produce various biochemicals with examples. Transcriptomic and metabolomic patterns of xylose-grown yeast cells were distinct from those on glucose—a conventional sugar of industrial biotechnology—and the gap might lead to opportunities to produce biochemicals efficiently. Indeed, limited glycolytic metabolic fluxes during xylose utilization could result in enhanced production of metabolites whose biosynthetic pathways compete for precursors with ethanol fermentation. Also, alleviation of glucose repression on cytosolic acetyl coenzyme A (acetyl-CoA) synthesis, and respiratory energy metabolism during xylose utilization enhanced production of acetyl-CoA derivatives. Consideration of singular properties of xylose metabolism, such as redox cofactor imbalance between xylose reductase and xylitol dehydrogenase, is necessary to maximize these positive xylose effects. This review argues the importance and benefits of xylose utilization as not only a way of expanding a substrate range, but also an effective environmental perturbation for the efficient production of advanced biofuels and chemicals in yeasts.},
doi = {10.1016/j.biotechadv.2018.12.003},
journal = {Biotechnology Advances},
issn = {0734-9750},
number = 2,
volume = 37,
place = {United States},
year = {2019},
month = {3}
}

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