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Title: Optical Polarization Based Genomic Sensor.

Abstract

Optical fluorescence-based DNA assays are commonly used for pathogen detection and consist of an optical substrate containing DNA capture molecules, binding of target RNA or DNA sequences, followed by detection of the hybridization event with a fluorescent probe. Though fluorescence detection can offer exquisite signal-to-background ratios, with high specificity, vast opportunities exist to improve current optical-based genomic sensing approaches. For these reasons, there is a clear need to explore alternative optical sensing paradigms to alleviate these restrictions. Bio-templated nanomaterial synthesis has become a powerful concept for developing new platforms for bio-sensing, as the biomolecule of interest can act as part of the sensing transducer mechanism. We explored the use of DNA origami structures to immobilize gold nanoparticles in very precise localized arrangements producing unique optical absorption properties with implications in novel DNA sensing schemes. We also explored the use of in-situ TEM as a novel characterization method for DNA-nanoparticle assemblies.

Authors:
; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1494351
Report Number(s):
SAND2015-8749
672307
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English

Citation Formats

Polsky, Ronen, Appelhans, Leah, Wheeler, David R., Jungjohann, Katherine Leigh, Hayes, Dulce C., Campbell, DeAnna Marie, Rudolph, Angela, Rivas, Rhiana, Zubelewicz, Michael Czeslaw, Shreve, Andrew, Graves, Steve, and Brozik, Susan. Optical Polarization Based Genomic Sensor.. United States: N. p., 2015. Web. doi:10.2172/1494351.
Polsky, Ronen, Appelhans, Leah, Wheeler, David R., Jungjohann, Katherine Leigh, Hayes, Dulce C., Campbell, DeAnna Marie, Rudolph, Angela, Rivas, Rhiana, Zubelewicz, Michael Czeslaw, Shreve, Andrew, Graves, Steve, & Brozik, Susan. Optical Polarization Based Genomic Sensor.. United States. doi:10.2172/1494351.
Polsky, Ronen, Appelhans, Leah, Wheeler, David R., Jungjohann, Katherine Leigh, Hayes, Dulce C., Campbell, DeAnna Marie, Rudolph, Angela, Rivas, Rhiana, Zubelewicz, Michael Czeslaw, Shreve, Andrew, Graves, Steve, and Brozik, Susan. Thu . "Optical Polarization Based Genomic Sensor.". United States. doi:10.2172/1494351. https://www.osti.gov/servlets/purl/1494351.
@article{osti_1494351,
title = {Optical Polarization Based Genomic Sensor.},
author = {Polsky, Ronen and Appelhans, Leah and Wheeler, David R. and Jungjohann, Katherine Leigh and Hayes, Dulce C. and Campbell, DeAnna Marie and Rudolph, Angela and Rivas, Rhiana and Zubelewicz, Michael Czeslaw and Shreve, Andrew and Graves, Steve and Brozik, Susan},
abstractNote = {Optical fluorescence-based DNA assays are commonly used for pathogen detection and consist of an optical substrate containing DNA capture molecules, binding of target RNA or DNA sequences, followed by detection of the hybridization event with a fluorescent probe. Though fluorescence detection can offer exquisite signal-to-background ratios, with high specificity, vast opportunities exist to improve current optical-based genomic sensing approaches. For these reasons, there is a clear need to explore alternative optical sensing paradigms to alleviate these restrictions. Bio-templated nanomaterial synthesis has become a powerful concept for developing new platforms for bio-sensing, as the biomolecule of interest can act as part of the sensing transducer mechanism. We explored the use of DNA origami structures to immobilize gold nanoparticles in very precise localized arrangements producing unique optical absorption properties with implications in novel DNA sensing schemes. We also explored the use of in-situ TEM as a novel characterization method for DNA-nanoparticle assemblies.},
doi = {10.2172/1494351},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {10}
}