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Title: Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli

Abstract

Aviation fuels are an important target of biofuels research due to their high market demand and competitive price. Isoprenoids have been demonstrated as good feedstocks for advanced renewable jet fuels with high energy density, high heat of combustion, and excellent cold-weather performance. In particular, sesquiterpene compounds (C 15), such as farnesene and bisabolene, have been identified as promising jet fuel candidates. In this study, we explored three sesquiterpenes—epi-isozizaene, pentalenene and α-isocomene—as novel jet fuel precursors. We performed a computational analysis to calculate the energy of combustion of these sesquiterpenes and found that their specific energies are comparable to commercial jet fuel A-1. Through heterologous MVA pathway expression and promoter engineering, we produced 727.9 mg/L epi-isozizaene, 780.3 mg/L pentalenene and 77.5 mg/L α-isocomene in Escherichia coli and 344 mg/L pentalenene in Saccharomyces cerevisiae. We also introduced a dynamic autoinduction system using previously identified FPP-responsive promoters for inducer-free production and managed to achieve comparable amounts of each compound. We produced tricyclic sesquiterpenes epi-isozizaene, pentalenene and α-isocomene, promising jet fuel feedstocks at high production titers, providing novel, sustainable alternatives to petroleum-based jet fuels.

Authors:
 [1];  [2];  [2];  [2];  [3];  [2];  [2];  [2];  [2];  [4];  [5];  [3]; ORCiD logo [2]
  1. Beijing Univ. of Chemical Technology (China). College of Life Science and Technology; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems & Engineering Division
  2. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems & Engineering Division
  3. Beijing Univ. of Chemical Technology (China). College of Life Science and Technology
  4. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems & Engineering Division; Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering
  5. Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems & Engineering Division; Univ. of California, Berkeley, CA (United States). Dept. of Bioengineering. Dept. of Chemical and Biomolecular Engineering; Technical Univ. of Denmark, Lyngby (Denmark). The Novo Nordisk Foundation Center for Biosustainability; Shenzhen Inst. for Advanced Technologies (China). Center for Synthetic Biochemistry
Publication Date:
Research Org.:
Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Beijing Univ. of Chemical Technology (China)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); National Natural Science Foundation of China (NNSFC); China Scholarship Council
OSTI Identifier:
1494090
Grant/Contract Number:  
AC02-05CH11231; 21390202
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 11; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; sesquiterpene; jet fuel; epi-isozizaene; pentalenene; α-isocomene; FPP-responsive promoter engineering

Citation Formats

Liu, Chun-Li, Tian, Tian, Alonso-Gutierrez, Jorge, Garabedian, Brett, Wang, Shuai, Baidoo, Edward E. K., Benites, Veronica, Chen, Yan, Petzold, Christopher J., Adams, Paul D., Keasling, Jay D., Tan, Tianwei, and Lee, Taek Soon. Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli. United States: N. p., 2018. Web. doi:10.1186/s13068-018-1272-z.
Liu, Chun-Li, Tian, Tian, Alonso-Gutierrez, Jorge, Garabedian, Brett, Wang, Shuai, Baidoo, Edward E. K., Benites, Veronica, Chen, Yan, Petzold, Christopher J., Adams, Paul D., Keasling, Jay D., Tan, Tianwei, & Lee, Taek Soon. Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli. United States. doi:10.1186/s13068-018-1272-z.
Liu, Chun-Li, Tian, Tian, Alonso-Gutierrez, Jorge, Garabedian, Brett, Wang, Shuai, Baidoo, Edward E. K., Benites, Veronica, Chen, Yan, Petzold, Christopher J., Adams, Paul D., Keasling, Jay D., Tan, Tianwei, and Lee, Taek Soon. Sat . "Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli". United States. doi:10.1186/s13068-018-1272-z. https://www.osti.gov/servlets/purl/1494090.
@article{osti_1494090,
title = {Renewable production of high density jet fuel precursor sesquiterpenes from Escherichia coli},
author = {Liu, Chun-Li and Tian, Tian and Alonso-Gutierrez, Jorge and Garabedian, Brett and Wang, Shuai and Baidoo, Edward E. K. and Benites, Veronica and Chen, Yan and Petzold, Christopher J. and Adams, Paul D. and Keasling, Jay D. and Tan, Tianwei and Lee, Taek Soon},
abstractNote = {Aviation fuels are an important target of biofuels research due to their high market demand and competitive price. Isoprenoids have been demonstrated as good feedstocks for advanced renewable jet fuels with high energy density, high heat of combustion, and excellent cold-weather performance. In particular, sesquiterpene compounds (C15), such as farnesene and bisabolene, have been identified as promising jet fuel candidates. In this study, we explored three sesquiterpenes—epi-isozizaene, pentalenene and α-isocomene—as novel jet fuel precursors. We performed a computational analysis to calculate the energy of combustion of these sesquiterpenes and found that their specific energies are comparable to commercial jet fuel A-1. Through heterologous MVA pathway expression and promoter engineering, we produced 727.9 mg/L epi-isozizaene, 780.3 mg/L pentalenene and 77.5 mg/L α-isocomene in Escherichia coli and 344 mg/L pentalenene in Saccharomyces cerevisiae. We also introduced a dynamic autoinduction system using previously identified FPP-responsive promoters for inducer-free production and managed to achieve comparable amounts of each compound. We produced tricyclic sesquiterpenes epi-isozizaene, pentalenene and α-isocomene, promising jet fuel feedstocks at high production titers, providing novel, sustainable alternatives to petroleum-based jet fuels.},
doi = {10.1186/s13068-018-1272-z},
journal = {Biotechnology for Biofuels},
issn = {1754-6834},
number = ,
volume = 11,
place = {United States},
year = {2018},
month = {10}
}

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