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Title: Amplification of biological targets via on-chip culture for biosensing

Abstract

The present invention, in part, relates to methods and apparatuses for on-chip amplification and/or detection of various targets, including biological targets and any amplifiable targets. In some examples, the microculture apparatus includes a single-use, normally-closed fluidic valve that is initially maintained in the closed position by a valve element bonded to an adhesive coating. The valve is opened using a magnetic force. The valve element includes a magnetic material or metal. Such apparatuses and methods are useful for in-field or real-time detection of targets, especially in limited resource settings.

Inventors:
; ; ; ;
Publication Date:
Research Org.:
Sandia National Laboratories (SNL), Albuquerque, NM, and Livermore, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1493615
Patent Number(s):
10,126,299
Application Number:
15/820,836
Assignee:
National Technology & Engineering Solutions of Sandia, LLC (Albuquerque, NM) SNL
DOE Contract Number:  
NA0003525
Resource Type:
Patent
Resource Relation:
Patent File Date: 2017 Nov 22
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Harper, Jason C., Edwards, Thayne L., Carson, Bryan, Finley, Melissa, and Arndt, William. Amplification of biological targets via on-chip culture for biosensing. United States: N. p., 2018. Web.
Harper, Jason C., Edwards, Thayne L., Carson, Bryan, Finley, Melissa, & Arndt, William. Amplification of biological targets via on-chip culture for biosensing. United States.
Harper, Jason C., Edwards, Thayne L., Carson, Bryan, Finley, Melissa, and Arndt, William. Tue . "Amplification of biological targets via on-chip culture for biosensing". United States. https://www.osti.gov/servlets/purl/1493615.
@article{osti_1493615,
title = {Amplification of biological targets via on-chip culture for biosensing},
author = {Harper, Jason C. and Edwards, Thayne L. and Carson, Bryan and Finley, Melissa and Arndt, William},
abstractNote = {The present invention, in part, relates to methods and apparatuses for on-chip amplification and/or detection of various targets, including biological targets and any amplifiable targets. In some examples, the microculture apparatus includes a single-use, normally-closed fluidic valve that is initially maintained in the closed position by a valve element bonded to an adhesive coating. The valve is opened using a magnetic force. The valve element includes a magnetic material or metal. Such apparatuses and methods are useful for in-field or real-time detection of targets, especially in limited resource settings.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {11}
}

Patent:

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Works referenced in this record:

Laser Machined Plastic Laminates: Towards Portable Diagnostic Devices for Use in Low Resource Environments
journal, July 2015

  • Harper, Jason C.; Carson, Bryan D.; Bachand, George D.
  • Electroanalysis, Vol. 27, Issue 11, p. 2503-2512
  • DOI: 10.1002/elan.201500359

Magnetic-adhesive based valves for microfluidic devices used in low-resource settings
journal, January 2016

  • Harper, Jason C.; Andrews, Jenna M.; Ben, Candice
  • Lab on a Chip, Vol. 16, Issue 21, p. 4142-4151
  • DOI: 10.1039/C6LC00858E

Point-of-care nucleic acid testing for infectious diseases
journal, May 2011


Electrically addressable diazonium-functionalized antibodies for multianalyte electrochemical sensor applications
journal, January 2008

  • Polsky, Ronen; Harper, Jason C.; Wheeler, David R.
  • Biosensors and Bioelectronics, Vol. 23, Issue 6, p. 757-764
  • DOI: 10.1016/j.bios.2007.08.013

Selective Immobilization of DNA and Antibody Probes on Electrode Arrays:� Simultaneous Electrochemical Detection of DNA and Protein on a Single Platform
journal, July 2007

  • Harper, Jason C.; Polsky, Ronen; Wheeler, David R.
  • Langmuir, Vol. 23, Issue 16, p. 8285-8287
  • DOI: 10.1021/la701775g