Short-chain ketone production by engineered polyketide synthases in Streptomyces albus
Abstract
Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Here we report engineered microbes that produce various short-chain (C5-C7) ketones using hybrid PKSs. Introduction of the genes into the chromosome of Streptomyces albus enables it to produce >1 g · l-1 of C6 and C7 ethyl ketones and several hundred mg · l-1 of C5 and C6 methyl ketones from plant biomass hydrolysates. Engine tests indicate these short-chain ketones can be added to gasoline as oxygenates to increase the octane of gasoline. Together, it demonstrates the efficient and renewable microbial production of biogasolines by hybrid enzymes.
- Authors:
-
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biogical Systems and Engineering Division; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of Tokyo (Japan). Biotechnology Research Center
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biogical Systems and Engineering Division, and Advanced Biofuels & Bioproducts Process Development Unit
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biogical Systems and Engineering Division
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); National Inst. of Advanced Industrial Science and Technology, Higashi-hiroshima, Hiroshima (Japan). Research Inst. for Sustainable Chemistry, Inst. for Synthetic Biology
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biogical Systems and Engineering Division; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States)
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Sandia National Lab. (SNL-CA), Livermore, CA (United States)
- Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). QB3 Inst.
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biogical Systems and Engineering Division; Joint BioEnergy Inst. (JBEI), Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). QB3 Inst., Dept. of Bioengineering, Dept. of Chemical and Biomolecular Engineering; Technical Univ. of Denmark, Lyngby (Denmark). Novo Nordisk Foundation Center for Biosustainability; Shenzhen Inst. for Advanced Technologies, Shenzhen, Guangdong (China). Center for Synthetic Biochemistry
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Science Foundation (NSF)
- OSTI Identifier:
- 1493265
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Journal Article: Accepted Manuscript
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Yuzawa, Satoshi, Mirsiaghi, Mona, Jocic, Renee, Fujii, Tatsuya, Masson, Fabrice, Benites, Veronica T., Baidoo, Edward E. K., Sundstrom, Eric, Tanjore, Deepti, Pray, Todd R., George, Anthe, Davis, Ryan W., Gladden, John M., Simmons, Blake A., Katz, Leonard, and Keasling, Jay D. Short-chain ketone production by engineered polyketide synthases in Streptomyces albus. United States: N. p., 2018.
Web. doi:10.1038/s41467-018-07040-0.
Yuzawa, Satoshi, Mirsiaghi, Mona, Jocic, Renee, Fujii, Tatsuya, Masson, Fabrice, Benites, Veronica T., Baidoo, Edward E. K., Sundstrom, Eric, Tanjore, Deepti, Pray, Todd R., George, Anthe, Davis, Ryan W., Gladden, John M., Simmons, Blake A., Katz, Leonard, & Keasling, Jay D. Short-chain ketone production by engineered polyketide synthases in Streptomyces albus. United States. https://doi.org/10.1038/s41467-018-07040-0
Yuzawa, Satoshi, Mirsiaghi, Mona, Jocic, Renee, Fujii, Tatsuya, Masson, Fabrice, Benites, Veronica T., Baidoo, Edward E. K., Sundstrom, Eric, Tanjore, Deepti, Pray, Todd R., George, Anthe, Davis, Ryan W., Gladden, John M., Simmons, Blake A., Katz, Leonard, and Keasling, Jay D. 2018.
"Short-chain ketone production by engineered polyketide synthases in Streptomyces albus". United States. https://doi.org/10.1038/s41467-018-07040-0. https://www.osti.gov/servlets/purl/1493265.
@article{osti_1493265,
title = {Short-chain ketone production by engineered polyketide synthases in Streptomyces albus},
author = {Yuzawa, Satoshi and Mirsiaghi, Mona and Jocic, Renee and Fujii, Tatsuya and Masson, Fabrice and Benites, Veronica T. and Baidoo, Edward E. K. and Sundstrom, Eric and Tanjore, Deepti and Pray, Todd R. and George, Anthe and Davis, Ryan W. and Gladden, John M. and Simmons, Blake A. and Katz, Leonard and Keasling, Jay D.},
abstractNote = {Microbial production of fuels and commodity chemicals has been performed primarily using natural or slightly modified enzymes, which inherently limits the types of molecules that can be produced. Type I modular polyketide synthases (PKSs) are multi-domain enzymes that can produce unique and diverse molecular structures by combining particular types of catalytic domains in a specific order. This catalytic mechanism offers a wealth of engineering opportunities. Here we report engineered microbes that produce various short-chain (C5-C7) ketones using hybrid PKSs. Introduction of the genes into the chromosome of Streptomyces albus enables it to produce >1 g · l-1 of C6 and C7 ethyl ketones and several hundred mg · l-1 of C5 and C6 methyl ketones from plant biomass hydrolysates. Engine tests indicate these short-chain ketones can be added to gasoline as oxygenates to increase the octane of gasoline. Together, it demonstrates the efficient and renewable microbial production of biogasolines by hybrid enzymes.},
doi = {10.1038/s41467-018-07040-0},
url = {https://www.osti.gov/biblio/1493265},
journal = {Nature Communications},
issn = {2041-1723},
number = 1,
volume = 9,
place = {United States},
year = {Thu Nov 01 00:00:00 EDT 2018},
month = {Thu Nov 01 00:00:00 EDT 2018}
}
Web of Science
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