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Title: Renewable diesel via hydrothermal liquefaction of oleaginous yeast and residual lignin from bioconversion of corn stover

Abstract

A major barrier to profitable bioconversion of cellulosic biomass to hydrocarbon fuel precursors is the difficulty in breaking down feedstock lignin into carbon substrates that can be easily metabolized by industrial microbes. Hydrothermal liquefaction (HTL) of cellulosic biomass effectively breaks down and converts lignin into biocrude oil products, but provides relatively low yields of biocrude from feedstock carbohydrates. In the present study, bioconversion and HTL were integrated into a new hybrid system that employs the best of both processes to produce high quality fuel in high quantity. Bioreactor cultivation of the oleaginous yeast Lypomyces starkeyi produced dry cell mass yields up to 0.43 g/g of sugar and yields of intracellular triglyceride lipids (measured as fatty acid methyl esters) of up to 0.26 g/g of sugar in pretreated corn stover hydrolysate or simulated hydrolysate media. The lipid-rich bioreactor broth was mixed with pretreated corn stover lignin, and then co-fed to an HTL reactor to produce 0.40 g of biocrude oil per gram of feed on a dry weight basis. Optimizing the separation of HTL biocrude and the HTL aqueous phase could significantly increase the biocrude yield. Hydrotreating of the biocrude produced a high quality, renewable hydrocarbon fuel blendstock, with the majoritymore » of the product boiling in the distillate range. The estimated cost of production could be reduced to $3/gasoline gallon equivalent through addressing identified research gaps. Our results provide a proof-of-concept for a new hybrid biorefinery design that could enhance domestic production of renewable diesel, jet, and marine fuel in the United States.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1492716
Report Number(s):
PNNL-SA-133222
Journal ID: ISSN 0306-2619
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Journal Name:
Applied Energy
Additional Journal Information:
Journal Volume: 233-234; Journal Issue: C; Journal ID: ISSN 0306-2619
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
Biomass, Continuous hydrothermal liquefaction, Biocrude, Oleaginous yeast, Lignin, Continuous hydrotreating

Citation Formats

Collett, James R., Billing, Justin M., Meyer, Pimphan A., Schmidt, Andrew J., Remington, A. Brook, Hawley, Erik R., Hofstad, Beth A., Panisko, Ellen A., Dai, Ziyu, Hart, Todd R., Santosa, Daniel M., Magnuson, Jon K., Hallen, Richard T., and Jones, Susanne B. Renewable diesel via hydrothermal liquefaction of oleaginous yeast and residual lignin from bioconversion of corn stover. United States: N. p., 2019. Web. doi:10.1016/j.apenergy.2018.09.115.
Collett, James R., Billing, Justin M., Meyer, Pimphan A., Schmidt, Andrew J., Remington, A. Brook, Hawley, Erik R., Hofstad, Beth A., Panisko, Ellen A., Dai, Ziyu, Hart, Todd R., Santosa, Daniel M., Magnuson, Jon K., Hallen, Richard T., & Jones, Susanne B. Renewable diesel via hydrothermal liquefaction of oleaginous yeast and residual lignin from bioconversion of corn stover. United States. doi:10.1016/j.apenergy.2018.09.115.
Collett, James R., Billing, Justin M., Meyer, Pimphan A., Schmidt, Andrew J., Remington, A. Brook, Hawley, Erik R., Hofstad, Beth A., Panisko, Ellen A., Dai, Ziyu, Hart, Todd R., Santosa, Daniel M., Magnuson, Jon K., Hallen, Richard T., and Jones, Susanne B. Tue . "Renewable diesel via hydrothermal liquefaction of oleaginous yeast and residual lignin from bioconversion of corn stover". United States. doi:10.1016/j.apenergy.2018.09.115.
@article{osti_1492716,
title = {Renewable diesel via hydrothermal liquefaction of oleaginous yeast and residual lignin from bioconversion of corn stover},
author = {Collett, James R. and Billing, Justin M. and Meyer, Pimphan A. and Schmidt, Andrew J. and Remington, A. Brook and Hawley, Erik R. and Hofstad, Beth A. and Panisko, Ellen A. and Dai, Ziyu and Hart, Todd R. and Santosa, Daniel M. and Magnuson, Jon K. and Hallen, Richard T. and Jones, Susanne B.},
abstractNote = {A major barrier to profitable bioconversion of cellulosic biomass to hydrocarbon fuel precursors is the difficulty in breaking down feedstock lignin into carbon substrates that can be easily metabolized by industrial microbes. Hydrothermal liquefaction (HTL) of cellulosic biomass effectively breaks down and converts lignin into biocrude oil products, but provides relatively low yields of biocrude from feedstock carbohydrates. In the present study, bioconversion and HTL were integrated into a new hybrid system that employs the best of both processes to produce high quality fuel in high quantity. Bioreactor cultivation of the oleaginous yeast Lypomyces starkeyi produced dry cell mass yields up to 0.43 g/g of sugar and yields of intracellular triglyceride lipids (measured as fatty acid methyl esters) of up to 0.26 g/g of sugar in pretreated corn stover hydrolysate or simulated hydrolysate media. The lipid-rich bioreactor broth was mixed with pretreated corn stover lignin, and then co-fed to an HTL reactor to produce 0.40 g of biocrude oil per gram of feed on a dry weight basis. Optimizing the separation of HTL biocrude and the HTL aqueous phase could significantly increase the biocrude yield. Hydrotreating of the biocrude produced a high quality, renewable hydrocarbon fuel blendstock, with the majority of the product boiling in the distillate range. The estimated cost of production could be reduced to $3/gasoline gallon equivalent through addressing identified research gaps. Our results provide a proof-of-concept for a new hybrid biorefinery design that could enhance domestic production of renewable diesel, jet, and marine fuel in the United States.},
doi = {10.1016/j.apenergy.2018.09.115},
journal = {Applied Energy},
issn = {0306-2619},
number = C,
volume = 233-234,
place = {United States},
year = {2019},
month = {1}
}