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Title: Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts

Abstract

Plant biomass is a promising carbon source for producing value-added chemicals, including transportation biofuels, polymer precursors, and various additives. Most engineered microbial hosts and a select group of wild-type species can metabolize mixed sugars including oligosaccharides, hexoses, and pentoses that are hydrolyzed from plant biomass. However, most of these microorganisms consume glucose preferentially to non-glucose sugars through mechanisms generally defined as carbon catabolite repression. The current lack of simultaneous mixed-sugar utilization limits achievable titers, yields, and productivities. Therefore, the development of microbial platforms capable of fermenting mixed sugars simultaneously from biomass hydrolysates is essential for economical industry-scale production, particularly for compounds with marginal profits. This review aims to summarize recent discoveries and breakthroughs in the engineering of yeast cell factories for improved mixed-sugar co-utilization based on various metabolic engineering approaches. Emphasis is placed on enhanced non-glucose utilization, discovery of novel sugar transporters free from glucose repression, native xylose-utilizing microbes, consolidated bioprocessing (CBP), improved cellulase secretion, and creation of microbial consortia for improving mixed-sugar utilization. Perspectives on the future development of biorenewables industry are provided in the end.

Authors:
; ;
Publication Date:
Research Org.:
Ames Lab., Ames, IA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1491605
Alternate Identifier(s):
OSTI ID: 1492340
Report Number(s):
IS-J-9840
Journal ID: ISSN 1664-302X; 3264
Grant/Contract Number:  
DE-AC02-07CH11358
Resource Type:
Journal Article: Published Article
Journal Name:
Frontiers in Microbiology
Additional Journal Information:
Journal Name: Frontiers in Microbiology Journal Volume: 9; Journal ID: ISSN 1664-302X
Publisher:
Frontiers Media SA
Country of Publication:
Switzerland
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Gao, Meirong, Ploessl, Deon, and Shao, Zengyi. Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts. Switzerland: N. p., 2019. Web. doi:10.3389/fmicb.2018.03264.
Gao, Meirong, Ploessl, Deon, & Shao, Zengyi. Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts. Switzerland. doi:10.3389/fmicb.2018.03264.
Gao, Meirong, Ploessl, Deon, and Shao, Zengyi. Tue . "Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts". Switzerland. doi:10.3389/fmicb.2018.03264.
@article{osti_1491605,
title = {Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts},
author = {Gao, Meirong and Ploessl, Deon and Shao, Zengyi},
abstractNote = {Plant biomass is a promising carbon source for producing value-added chemicals, including transportation biofuels, polymer precursors, and various additives. Most engineered microbial hosts and a select group of wild-type species can metabolize mixed sugars including oligosaccharides, hexoses, and pentoses that are hydrolyzed from plant biomass. However, most of these microorganisms consume glucose preferentially to non-glucose sugars through mechanisms generally defined as carbon catabolite repression. The current lack of simultaneous mixed-sugar utilization limits achievable titers, yields, and productivities. Therefore, the development of microbial platforms capable of fermenting mixed sugars simultaneously from biomass hydrolysates is essential for economical industry-scale production, particularly for compounds with marginal profits. This review aims to summarize recent discoveries and breakthroughs in the engineering of yeast cell factories for improved mixed-sugar co-utilization based on various metabolic engineering approaches. Emphasis is placed on enhanced non-glucose utilization, discovery of novel sugar transporters free from glucose repression, native xylose-utilizing microbes, consolidated bioprocessing (CBP), improved cellulase secretion, and creation of microbial consortia for improving mixed-sugar utilization. Perspectives on the future development of biorenewables industry are provided in the end.},
doi = {10.3389/fmicb.2018.03264},
journal = {Frontiers in Microbiology},
issn = {1664-302X},
number = ,
volume = 9,
place = {Switzerland},
year = {2019},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record at 10.3389/fmicb.2018.03264

Citation Metrics:
Cited by: 5 works
Citation information provided by
Web of Science

Figures / Tables:

Figure 1 Figure 1: Carbohydrate metabolism in microorganisms. Red dotted line corresponds to inhibition. Abbreviation of metabolites—PEP, phosphoenolpyruvate; G6P, glucose-6-phosphate; 6-PGL, 6-phosphogluconolactone; 6-PGC, 6-phosphogluconate; D-Ri5P, D-ribulose-5-phosphate; D-X5P, D-xylulose-5-phosphate; R5P, ribose-5-phosphate; G3P, glyceraldehyde-3-phosphate; S7P, sedoheptulose-7-phosphate; F6P, fructose-6-phosphate; E4P, erythrose-4-phosphate; L-Ri5P, L-ribulose-5-phosphate. Abbreviation of enzymes—BGL, b-glucosidase; HXK, hexokinase; PYK, pyruvate kinase; PDC, pyruvate decarboxylase;more » ADH, alcohol dehydrogenase; ZWF, glucose-6-phosphate dehydrogenase; 6PGL, 6-phosphogluconolactonase; GND, 6-phosphogluconate dehydrogenase; RPI, ribose-5-phosphate isomerase; RPE, ribulose-5-phosphate epimerase; TKT, transketolase; TAL, transaldolase; XR, xylose reductase; XDH, xylose dehydrogenase; XKS, xylulokinase; XI/XylA, xylose isomerase; LAD, L-arabitol 4-dehydrogenase; LXR, L-xylulose reductase; AraA, L-arabinose isomerase; AraB, L-ribulokinase; AraD, L-ribulose-5-phosphate 4-epimerase.« less

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Main and interaction effects of acetic acid, furfural, andp-hydroxybenzoic acid on growth and ethanol productivity of yeasts
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Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway
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CRISPR-Mediated Genome Editing and Gene Repression in Scheffersomyces stipitis
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The genetic code of the fungal CTG clade
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Efficient bioethanol production from xylose by recombinant saccharomyces cerevisiae requires high activity of xylose reductase and moderate xylulokinase activity
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Opportunities and challenges for a sustainable energy future
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The genome sequence of the popular hexose-transport-deficient Saccharomyces cerevisiae strain EBY.VW4000 reveals LoxP/Cre-induced translocations and gene loss
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Co-fermentation of xylose and cellobiose by an engineered Saccharomyces cerevisiae
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Recent progress in consolidated bioprocessing
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The xylose reductase/xylitol dehydrogenase/xylulokinase ratio affects product formation in recombinant xylose-utilising Saccharomyces cerevisiae
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Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering
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Challenges and advances in the heterologous expression of cellulolytic enzymes: a review
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Highly efficient conversion of xylose to ethanol without glucose repression by newly isolated thermotolerant Spathaspora passalidarum CMUWF1–2
journal, July 2018

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Functional Survey for Heterologous Sugar Transport Proteins, Using Saccharomyces cerevisiae as a Host
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Decreased Xylitol Formation during Xylose Fermentation in Saccharomyces cerevisiae Due to Overexpression of Water-Forming NADH Oxidase
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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
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Cofermentation of Cellobiose and Galactose by an Engineered Saccharomyces cerevisiae Strain
journal, June 2011

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Locking out viral replication
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Complete Reversal of Coenzyme Specificity of Xylitol Dehydrogenase and Increase of Thermostability by the Introduction of Structural Zinc
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Employing a combinatorial expression approach to characterize xylose utilization in Saccharomyces cerevisiae
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The expression of aPichia stipitis xylose reductase mutant with higherKM for NADPH increases ethanol production from xylose in recombinantSaccharomyces cerevisiae
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The YGR194c (XKS1) gene encodes the xylulokinase from the budding yeast Saccharomyces cerevisiae
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Screening of transporters to improve xylodextrin utilization in the yeast Saccharomyces cerevisiae
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Xylose metabolism in the anaerobic fungus Piromyces sp. strain E2 follows the bacterial pathway
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Developing symbiotic consortia for lignocellulosic biofuel production
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Engineering of Saccharomyces cerevisiae for Efficient Anaerobic Alcoholic Fermentation of L-Arabinose
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Expression of Arabidopsis thaliana xylose isomerase gene and its effect on ethanol production in Flammulina velutipes
journal, November 2013


Optimal Growth and Ethanol Production from Xylose by Recombinant Saccharomyces cerevisiae Require Moderate D-Xylulokinase Activity
journal, January 2003


Effective xylose/cellobiose co-fermentation and ethanol production by xylose-assimilating S. cerevisiae via expression of β-glucosidase on its cell surface
journal, September 2008


CRISPR-based genomic tools for the manipulation of genetically intractable microorganisms
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Engineered Saccharomyces cerevisiae capable of simultaneous cellobiose and xylose fermentation
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Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiae
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A co-fermentation strategy to consume sugar mixtures effectively
journal, January 2008

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Metabolic engineering of a xylose-isomerase-expressing strain for rapid anaerobic xylose fermentation
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Engineering for biofuels: exploiting innate microbial capacity or importing biosynthetic potential?
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Fine-tuning of NADH oxidase decreases byproduct accumulation in respiration deficient xylose metabolic Saccharomyces cerevisiae
journal, January 2014


Construction of a Xylan-Fermenting Yeast Strain through Codisplay of Xylanolytic Enzymes on the Surface of Xylose-Utilizing Saccharomyces cerevisiae Cells
journal, September 2004


Amino Acid Substitutions in the Yeast Pichia Stipitis Xylitol Dehydrogenase Coenzyme-Binding Domain Affect the Coenzyme Specificity
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Expression of E. coli araBAD operon encoding enzymes for metabolizing L-arabinose in Saccharomyces cerevisiae
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Cofermentation of glucose and xylose to ethanol by a respiratory-deficient mutant of Saccharomyces cerevisiae co-cultivated with a xylose-fermenting yeast
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An engineered cryptic Hxt11 sugar transporter facilitates glucose–xylose co-consumption in Saccharomyces cerevisiae
journal, November 2015

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An Efficient Genome-Wide Fusion Partner Screening System for Secretion of Recombinant Proteins in Yeast
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Xylose fermentation by Saccharomyces cerevisiae
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Rapid Isolation of Centromeres from Scheffersomyces stipitis
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Lipid production through simultaneous utilization of glucose, xylose, and l-arabinose by Pseudozyma hubeiensis: a comparative screening study
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Centromeric DNA Facilitates Nonconventional Yeast Genetic Engineering
journal, April 2017

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Dynamic metabolic modeling of a microaerobic yeast co-culture: predicting and optimizing ethanol production from glucose/xylose mixtures
journal, January 2013

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The Missing Link in the Fungal l -Arabinose Catabolic Pathway, Identification of the l -Xylulose Reductase Gene ,
journal, May 2002

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Genome and metabolic engineering in non-conventional yeasts: Current advances and applications
journal, September 2017


Ethanol production from a mixture of glucose and xylose by co-culture of Pichia stipitis and a respiratory-deficient mutant of Saccharomyces cerevisiae
journal, January 1997

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Systems analysis of plant cell wall degradation by the model filamentous fungus Neurospora crassa
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Endogenous Xylose Pathway in Saccharomyces cerevisiae
journal, June 2004


A Novel NADH-linked L-Xylulose Reductase in the L-Arabinose Catabolic Pathway of Yeast
journal, January 2004

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A molecular transporter engineering approach to improving xylose catabolism in Saccharomyces cerevisiae
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    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.