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Title: Chicago Area Waterway System Microbiome Research

Abstract

This report summarizes the research conducted by Argonne National Laboratory (Argonne) for the Metropolitan Water Reclamation District of Greater Chicago (MWRD) for the first five years (2013-2017) of a seven-year study with emphasis on the impact of disinfection (2016-2017) on the microbial community of the Chicago Area Waterway System (CAWS). The study examines microbial communities following disinfection of secondary treated effluents at the T.J. O’Brien (UV) and Calumet (chlorine and dechlorination) Water Reclamation Plants (WRPs) and the phased implementation of the Tunnel and Reservoir Plan (TARP). The Thornton Composite Reservoir (TCR) was completed in 2015. It provides 7.9 billion gallons of storage and since its completion has captured more than 11.0 billion gallons of combined stormwater and sewage from Calumet WRP that would otherwise overflow into CAWS in rainy weather. In addition to the effects of TARP and disinfection, we present an analysis of potential sources of the microbes found in the CAWS and the results of 16S rRNA amplicon gene sequencing which was used to characterize microbial community variability as a function of location, season, and environmental conditions. A total of 2,077 samples were collected from 2013 to 2017 from WRPs and CAWS and analyzed using high-throughput sequencing. Themore » whole microbial community composition using all the genetic material in the environmental sample was characterized using 16S rRNA gene-based analysis, while functional subsystem attributes of these microbial communities were characterized using shotgun metagenomics. We used a Bayesian statistical tool, SourceTracker 2.0 to determine the likely sources of microbes found in CAWS by sampling location and sampling period.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Argonne National Lab. (ANL), Argonne, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1490851
Report Number(s):
ANL/EVS-18/6
148882
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Technical Report
Country of Publication:
United States
Language:
English

Citation Formats

Negri, M. Cristina, Gilbert, Jack A., Grippo, Mark, Sharma, Anukriti, Dsouza, Melissa, Cacho, Jules F, and Campbell, Patty. Chicago Area Waterway System Microbiome Research. United States: N. p., 2018. Web. doi:10.2172/1490851.
Negri, M. Cristina, Gilbert, Jack A., Grippo, Mark, Sharma, Anukriti, Dsouza, Melissa, Cacho, Jules F, & Campbell, Patty. Chicago Area Waterway System Microbiome Research. United States. doi:10.2172/1490851.
Negri, M. Cristina, Gilbert, Jack A., Grippo, Mark, Sharma, Anukriti, Dsouza, Melissa, Cacho, Jules F, and Campbell, Patty. Mon . "Chicago Area Waterway System Microbiome Research". United States. doi:10.2172/1490851. https://www.osti.gov/servlets/purl/1490851.
@article{osti_1490851,
title = {Chicago Area Waterway System Microbiome Research},
author = {Negri, M. Cristina and Gilbert, Jack A. and Grippo, Mark and Sharma, Anukriti and Dsouza, Melissa and Cacho, Jules F and Campbell, Patty},
abstractNote = {This report summarizes the research conducted by Argonne National Laboratory (Argonne) for the Metropolitan Water Reclamation District of Greater Chicago (MWRD) for the first five years (2013-2017) of a seven-year study with emphasis on the impact of disinfection (2016-2017) on the microbial community of the Chicago Area Waterway System (CAWS). The study examines microbial communities following disinfection of secondary treated effluents at the T.J. O’Brien (UV) and Calumet (chlorine and dechlorination) Water Reclamation Plants (WRPs) and the phased implementation of the Tunnel and Reservoir Plan (TARP). The Thornton Composite Reservoir (TCR) was completed in 2015. It provides 7.9 billion gallons of storage and since its completion has captured more than 11.0 billion gallons of combined stormwater and sewage from Calumet WRP that would otherwise overflow into CAWS in rainy weather. In addition to the effects of TARP and disinfection, we present an analysis of potential sources of the microbes found in the CAWS and the results of 16S rRNA amplicon gene sequencing which was used to characterize microbial community variability as a function of location, season, and environmental conditions. A total of 2,077 samples were collected from 2013 to 2017 from WRPs and CAWS and analyzed using high-throughput sequencing. The whole microbial community composition using all the genetic material in the environmental sample was characterized using 16S rRNA gene-based analysis, while functional subsystem attributes of these microbial communities were characterized using shotgun metagenomics. We used a Bayesian statistical tool, SourceTracker 2.0 to determine the likely sources of microbes found in CAWS by sampling location and sampling period.},
doi = {10.2172/1490851},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {1}
}

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