Active-site plasticity revealed in the asymmetric dimer of AnPrx6 the 1-Cys peroxiredoxin and molecular chaperone from Anabaena sp. PCC 7120

Mishra, Yogesh; Hall, Michael; Locmelis, Roland; Nam, Kwangho; Söderberg, Christopher A.  G.; Storm, Patrik; Chaurasia, Neha; Rai, Lal Chand; Jansson, Stefan; Schröder, Wolfgang P.; Sauer, Uwe H.

doi:10.1038/s41598-017-17044-3

Title: Active-site plasticity revealed in the asymmetric dimer of AnPrx6 the 1-Cys peroxiredoxin and molecular chaperone from Anabaena sp. PCC 7120

Journal Article · Thu Dec 07 00:00:00 EST 2017 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-017-17044-3· OSTI ID:1490797

Mishra, Yogesh ^[1]; Hall, Michael ^[2]; Locmelis, Roland ^[2]; Nam, Kwangho ^[3]; Söderberg, Christopher A. G. ^[4]; Storm, Patrik ^[2]; Chaurasia, Neha ^[5]; Rai, Lal Chand ^[6]; Jansson, Stefan ^[7]; Schröder, Wolfgang P. ^[1]; Sauer, Uwe H. ^[8]

Umea Univ. (Sweden). Dept. of Chemistry and Umea Plant Science Center
Umea Univ. (Sweden). Dept. of Chemistry
Umea Univ. (Sweden). Dept. of Chemistry and Computational Life-Science Cluster (CLiC); Univ. of Texas, Arlington, TX (United States). Dept. of Chemistry and Biochemistry
Lund Univ. (Sweden). MAX IV Lab.
North Eastern Hill Univ., Shillong (India). Dept. of Biotechnology and Bioinformatics
Banaras Hindu Univ, Varanasi (India). Molecular Biology Section
Umea Univ. (Sweden). Umea Plant Science Center
Umea Univ. (Sweden). Dept. of Chemistry and Computational Life-Science Cluster (CLiC)

Peroxiredoxins (Prxs) are vital regulators of intracellular reactive oxygen species levels in all living organisms. Their activity depends on one or two catalytically active cysteine residues, the peroxidatic Cys (C_P) and, if present, the resolving Cys (C_R). A detailed catalytic cycle has been derived for typical 2-Cys Prxs, however, little is known about the catalytic cycle of 1-Cys Prxs. We have characterized Prx6 from the cyanobacterium Anabaena sp. strain PCC7120 (AnPrx6) and found that in addition to the expected peroxidase activity, AnPrx6 can act as a molecular chaperone in its dimeric state, contrary to other Prxs. The AnPrx6 crystal structure at 2.3 Å resolution reveals different active site conformations in each monomer of the asymmetric obligate homo-dimer. Molecular dynamic simulations support the observed structural plasticity. A FSH motif, conserved in 1-Cys Prxs, precedes the active site PxxxTxxCp signature and might contribute to the 1-Cys Prx reaction cycle.

View Accepted Manuscript (DOE)

Cite

Export

Save

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: USDOE

OSTI ID:: 1490797

Journal Information:: Scientific Reports, Vol. 7, Issue 1; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

Citation Metrics:

Cited by: 6 works

Citation information provided by
Web of Science

References (89)

Genome-scale protein expression and structural biology of Plasmodium falciparum and related Apicomplexan organisms Vedadi, Masoud; Lew, Jocelyne; Artz, Jennifer Molecular and Biochemical Parasitology, Vol. 151, Issue 1 https://doi.org/10.1016/j.molbiopara.2006.10.011	journal	January 2007
Peroxiredoxin 6: A Bifunctional Enzyme with Glutathione Peroxidase and Phospholipase A ₂ Activities Fisher, Aron B. Antioxidants & Redox Signaling, Vol. 15, Issue 3 https://doi.org/10.1089/ars.2010.3412	journal	August 2011
Peroxiredoxin Evolution and the Regulation of Hydrogen Peroxide Signaling Wood, Z. A. Science, Vol. 300, Issue 5619 https://doi.org/10.1126/science.1080405	journal	April 2003
Presenting your structures: the CCP 4 mg molecular-graphics software McNicholas, S.; Potterton, E.; Wilson, K. S. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444911007281	journal	March 2011
Peroxiredoxins in Plants and Cyanobacteria Dietz, Karl-Josef Antioxidants & Redox Signaling, Vol. 15, Issue 4 https://doi.org/10.1089/ars.2010.3657	journal	August 2011
Multiple Redox and Non-Redox Interactions Define 2-Cys Peroxiredoxin as a Regulatory Hub in the Chloroplast Muthuramalingam, Meenakumari; Seidel, Thorsten; Laxa, Miriam Molecular Plant, Vol. 2, Issue 6 https://doi.org/10.1093/mp/ssp089	journal	November 2009
Peroxiredoxins Hofmann, B.; Hecht, H. -J.; Flohé, L. Biological Chemistry, Vol. 383, Issue 3-4 https://doi.org/10.1515/bc.2002.040	journal	January 2002
Alkyl Hydroperoxide Reductase Is the Primary Scavenger of Endogenous Hydrogen Peroxide in Escherichia coli Seaver, L. C.; Imlay, J. A. Journal of Bacteriology, Vol. 183, Issue 24 https://doi.org/10.1128/JB.183.24.7173-7181.2001	journal	December 2001
Irreversible Oxidation of the Active-site Cysteine of Peroxiredoxin to Cysteine Sulfonic Acid for Enhanced Molecular Chaperone Activity Lim, Jung Chae; Choi, Hoon-In; Park, Yu Sun Journal of Biological Chemistry, Vol. 283, Issue 43 https://doi.org/10.1074/jbc.M804087200	journal	August 2008
Overview of the CCP 4 suite and current developments Winn, Martyn D.; Ballard, Charles C.; Cowtan, Kevin D. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S0907444910045749	journal	March 2011
Peroxiredoxins, gerontogenes linking aging to genome instability and cancer Nystrom, T.; Yang, J.; Molin, M. Genes & Development, Vol. 26, Issue 18 https://doi.org/10.1101/gad.200006.112	journal	September 2012
Evolution of the Peroxiredoxins Knoops, Bernard; Loumaye, ElÉonore; Van Der Eecken, ValÉrie Subcellular Biochemistry https://doi.org/10.1007/978-1-4020-6051-9_2	book	January 2007
Auto-Rickshaw : an automated crystal structure determination platform as an efficient tool for the validation of an X-ray diffraction experiment Panjikar, Santosh; Parthasarathy, Venkataraman; Lamzin, Victor S. Acta Crystallographica Section D Biological Crystallography, Vol. 61, Issue 4 https://doi.org/10.1107/S0907444905001307	journal	March 2005
Peroxiredoxin family proteins are key initiators of post-ischemic inflammation in the brain Shichita, Takashi; Hasegawa, Eiichi; Kimura, Akihiro Nature Medicine, Vol. 18, Issue 6 https://doi.org/10.1038/nm.2749	journal	May 2012
The Structure of Reduced Tryparedoxin Peroxidase Reveals a Decamer and Insight into Reactivity of 2Cys-peroxiredoxins Alphey, Magnus S.; Bond, Charles S.; Tetaud, Emmanuel Journal of Molecular Biology, Vol. 300, Issue 4 https://doi.org/10.1006/jmbi.2000.3881	journal	July 2000
Towards automated crystallographic structure refinement with phenix.refine Afonine, Pavel V.; Grosse-Kunstleve, Ralf W.; Echols, Nathaniel Acta Crystallographica Section D Biological Crystallography, Vol. 68, Issue 4 https://doi.org/10.1107/S0907444912001308	journal	March 2012
Comparison of simple potential functions for simulating liquid water Jorgensen, William L.; Chandrasekhar, Jayaraman; Madura, Jeffry D. The Journal of Chemical Physics, Vol. 79, Issue 2 https://doi.org/10.1063/1.445869	journal	July 1983
Crystal Structure of an Archaeal Peroxiredoxin from the Aerobic Hyperthermophilic Crenarchaeon Aeropyrum pernix K1 Mizohata, Eiichi; Sakai, Hiroaki; Fusatomi, Emiko Journal of Molecular Biology, Vol. 354, Issue 2 https://doi.org/10.1016/j.jmb.2005.09.006	journal	November 2005
Crystal structures of human peroxiredoxin 6 in different oxidation states Kim, Kyung Hee; Lee, Weontae; Kim, Eunice EunKyeong Biochemical and Biophysical Research Communications, Vol. 477, Issue 4 https://doi.org/10.1016/j.bbrc.2016.06.125	journal	September 2016
Structure-based Insights into the Catalytic Power and Conformational Dexterity of Peroxiredoxins Hall, Andrea; Nelson, Kimberly; Poole, Leslie B. Antioxidants & Redox Signaling, Vol. 15, Issue 3 https://doi.org/10.1089/ars.2010.3624	journal	August 2011
Do aligned sequences share the same fold? Abagyan, Ruben A.; Batalov, Serge Journal of Molecular Biology, Vol. 273, Issue 1 https://doi.org/10.1006/jmbi.1997.1287	journal	October 1997
Purification and characterization of the chaperone-like Hsp26 from Saccharomyces cerevisiae Ferreira, Renato Marins; de Andrade, Leonardo Rodrigues; Dutra, Márcio Barros Protein Expression and Purification, Vol. 47, Issue 2 https://doi.org/10.1016/j.pep.2006.02.006	journal	June 2006
Peroxiredoxin 1 and its role in cell signaling Neumann, Carola A.; Cao, Juxiang; Manevich, Yefim Cell Cycle, Vol. 8, Issue 24 https://doi.org/10.4161/cc.8.24.10242	journal	December 2009
Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega Sievers, Fabian; Wilm, Andreas; Dineen, David Molecular Systems Biology, Vol. 7, Issue 1 https://doi.org/10.1038/msb.2011.75	journal	January 2011
Structural and Electrostatic Asymmetry at the Active Site in Typical and Atypical Peroxiredoxin Dimers Salsbury, Freddie R.; Yuan, Ye; Knaggs, Michael H. The Journal of Physical Chemistry B, Vol. 116, Issue 23 https://doi.org/10.1021/jp212606k	journal	April 2012
All-Atom Empirical Potential for Molecular Modeling and Dynamics Studies of Proteins ^† MacKerell, A. D.; Bashford, D.; Bellott, M. The Journal of Physical Chemistry B, Vol. 102, Issue 18 https://doi.org/10.1021/jp973084f	journal	April 1998
Crystal structure of the tryparedoxin peroxidase from the human parasite Pineyro, M.; Pizarro, J.; Lema, F. Journal of Structural Biology, Vol. 150, Issue 1 https://doi.org/10.1016/j.jsb.2004.12.005	journal	April 2005
Physiological Relevance of Antioxid/Redox Genes; Learning from Genetically Modified Animals Guest Editor: Junichi Fujii Novel roles of peroxiredoxins in inflammation, cancer and innate immunity Ishii, Tetsuro; Warabi, Eiji; Yanagawa, Toru Journal of Clinical Biochemistry and Nutrition, Vol. 50, Issue 2 https://doi.org/10.3164/jcbn.11-109	journal	January 2012
Cysteine p K _a Values for the Bacterial Peroxiredoxin AhpC ^† ^‡ Nelson, Kimberly J.; Parsonage, Derek; Hall, Andrea Biochemistry, Vol. 47, Issue 48 https://doi.org/10.1021/bi801718d	journal	December 2008
The ID23-1 structural biology beamline at the ESRF Nurizzo, Didier; Mairs, Trevor; Guijarro, Matias Journal of Synchrotron Radiation, Vol. 13, Issue 3 https://doi.org/10.1107/s0909049506004341	journal	April 2006
The effect of acidic pH on the adsorption and lytic activity of the peptides Polybia-MP1 and its histidine-containing analog in anionic lipid membrane: a biophysical study by molecular dynamics and spectroscopy Martins, Ingrid Bernardes Santana; Viegas, Taisa Giordano; dos Santos Alvares, Dayane Amino Acids, Vol. 53, Issue 5 https://doi.org/10.1007/s00726-021-02982-0	journal	April 2021
Dimers to Doughnuts: Redox-Sensitive Oligomerization of 2-Cysteine Peroxiredoxins ^† ^, ^‡ Wood, Zachary A.; Poole, Leslie B.; Hantgan, Roy R. Biochemistry, Vol. 41, Issue 17 https://doi.org/10.1021/bi012173m	journal	April 2002
Effects of overexpression of the alkyl hydroperoxide reductase AhpC on the virulence and isoniazid resistance of Mycobacterium tuberculosis Heym, B.; Stavropoulos, E.; Honoré, N. Infection and Immunity, Vol. 65, Issue 4 https://doi.org/10.1128/iai.65.4.1395-1401.1997	journal	April 1997
CHARMM: The biomolecular simulation program Brooks, B. R.; Brooks, C. L.; Mackerell, A. D. Journal of Computational Chemistry, Vol. 30, Issue 10 https://doi.org/10.1002/jcc.21287	journal	July 2009
Structure, mechanism and regulation of peroxiredoxins Wood, Zachary A.; Schröder, Ewald; Robin Harris, J. Trends in Biochemical Sciences, Vol. 28, Issue 1 https://doi.org/10.1016/S0968-0004(02)00003-8	journal	January 2003
An NADH-Dependent Bacterial Thioredoxin Reductase-like Protein in Conjunction with a Glutaredoxin Homologue Form a Unique Peroxiredoxin (AhpC) Reducing System in Clostridium pasteurianum ^† Reynolds, C. Michael; Meyer, Jacques; Poole, Leslie B. Biochemistry, Vol. 41, Issue 6 https://doi.org/10.1021/bi011802p	journal	February 2002
Inference of Macromolecular Assemblies from Crystalline State Krissinel, Evgeny; Henrick, Kim Journal of Molecular Biology, Vol. 372, Issue 3 https://doi.org/10.1016/j.jmb.2007.05.022	journal	September 2007
XDS Kabsch, Wolfgang Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 2 https://doi.org/10.1107/S0907444909047337	journal	January 2010
Extending the treatment of backbone energetics in protein force fields: Limitations of gas-phase quantum mechanics in reproducing protein conformational distributions in molecular dynamics simulations Mackerell, Alexander D.; Feig, Michael; Brooks, Charles L. Journal of Computational Chemistry, Vol. 25, Issue 11 https://doi.org/10.1002/jcc.20065	journal	August 2004
Cloning and sequencing of thiol-specific antioxidant from mammalian brain: alkyl hydroperoxide reductase and thiol-specific antioxidant define a large family of antioxidant enzymes. Chae, H. Z.; Robison, K.; Poole, L. B. Proceedings of the National Academy of Sciences, Vol. 91, Issue 15 https://doi.org/10.1073/pnas.91.15.7017	journal	July 1994
Proteomic evaluation of the non-survival of Anabaena doliolum (Cyanophyta) at elevated temperatures Mishra, Yogesh; Bhargava, Poonam; Chaurasia, Neha European Journal of Phycology, Vol. 44, Issue 4 https://doi.org/10.1080/09670260902947001	journal	November 2009
Identification of a human cDNA clone for lysosomal type Ca2+-independent phospholipase A2 and properties of the expressed protein. Kim, Tae-Suk; Sundaresh, Chennarayapatna S.; Feinstein, Sheldon I. Journal of Biological Chemistry, Vol. 272, Issue 16 https://doi.org/10.1016/s0021-9258(18)40782-x	journal	April 1997
Deconstructing the Catalytic Efficiency of Peroxiredoxin-5 Peroxidatic Cysteine Portillo-Ledesma, Stephanie; Sardi, Florencia; Manta, Bruno Biochemistry, Vol. 53, Issue 38 https://doi.org/10.1021/bi500389m	journal	September 2014
NCBI Reference Sequence (RefSeq): a curated non-redundant sequence database of genomes, transcripts and proteins Pruitt, K. D. Nucleic Acids Research, Vol. 33, Issue Database issue https://doi.org/10.1093/nar/gki025	journal	December 2004
Activation of the antioxidant enzyme 1-CYS peroxiredoxin requires glutathionylation mediated by heterodimerization with GST Manevich, Y.; Feinstein, S. I.; Fisher, A. B. Proceedings of the National Academy of Sciences, Vol. 101, Issue 11 https://doi.org/10.1073/pnas.0400181101	journal	March 2004
Peroxiredoxins are conserved markers of circadian rhythms Edgar, Rachel S.; Green, Edward W.; Zhao, Yuwei Nature, Vol. 485, Issue 7399 https://doi.org/10.1038/nature11088	journal	May 2012
The Crystal Structures of the Tryparedoxin-Tryparedoxin Peroxidase Couple Unveil the Structural Determinants of Leishmania Detoxification Pathway Fiorillo, Annarita; Colotti, Gianni; Boffi, Alberto PLoS Neglected Tropical Diseases, Vol. 6, Issue 8 https://doi.org/10.1371/journal.pntd.0001781	journal	August 2012
Structure of peroxiredoxin from the anaerobic hyperthermophilic archaeon Pyrococcus horikoshii Nakamura, Tsutomu; Mori, Aika; Niiyama, Mayumi Acta Crystallographica Section F Structural Biology and Crystallization Communications, Vol. 69, Issue 7 https://doi.org/10.1107/S1744309113014036	journal	June 2013
Two Enzymes in One Jang, Ho Hee; Lee, Kyun Oh; Chi, Yong Hun Cell, Vol. 117, Issue 5 https://doi.org/10.1016/j.cell.2004.05.002	journal	May 2004
Crystal structure of a novel human peroxidase enzyme at 2.0 Å resolution Choi, Hee-Jeong; Kang, Sang Won; Yang, Chul-Hak Nature structural biology, Vol. 5, Issue 5 https://doi.org/10.1038/nsb0598-400	journal	May 1998
CHARMM general force field: A force field for drug-like molecules compatible with the CHARMM all-atom additive biological force fields Vanommeslaeghe, K.; Hatcher, E.; Acharya, C. Journal of Computational Chemistry https://doi.org/10.1002/jcc.21367	journal	January 2009
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
An introduction to data reduction: space-group determination, scaling and intensity statistics Evans, Philip R. Acta Crystallographica Section D Biological Crystallography, Vol. 67, Issue 4 https://doi.org/10.1107/S090744491003982X	journal	March 2011
Conformational and Oligomeric Effects on the Cysteine pK ^a of Tryparedoxin Peroxidase Yuan, Ye; Knaggs, Michael H.; Poole, Leslie B. Journal of Biomolecular Structure and Dynamics, Vol. 28, Issue 1 https://doi.org/10.1080/07391102.2010.10507343	journal	August 2010
The Roles of Peroxidase and Phospholipase A ₂ Activities of Peroxiredoxin 6 in Protecting Pulmonary Microvascular Endothelial Cells Against Peroxidative Stress Lien, Yu-Chin; Feinstein, Sheldon I.; Dodia, Chandra Antioxidants & Redox Signaling, Vol. 16, Issue 5 https://doi.org/10.1089/ars.2011.3950	journal	March 2012
The antioxidant protein alkylhydroperoxide reductase of Helicobacter pylori switches from a peroxide reductase to a molecular chaperone function Chuang, M. -H.; Wu, M. -S.; Lo, W. -L. Proceedings of the National Academy of Sciences, Vol. 103, Issue 8 https://doi.org/10.1073/pnas.0510770103	journal	February 2006
Expression, purification, crystallization and preliminary X-ray crystallographic studies of alkyl hydroperoxide reductase (AhpC) from the cyanobacterium Anabaena sp. PCC 7120 Mishra, Yogesh; Hall, Michael; Chaurasia, Neha Acta Crystallographica Section F Structural Biology and Crystallization Communications, Vol. 67, Issue 10 https://doi.org/10.1107/s1744309111025747	journal	September 2011
The Protein Data Bank Berman, H. M. Nucleic Acids Research, Vol. 28, Issue 1 https://doi.org/10.1093/nar/28.1.235	journal	January 2000
The crystal structure of the C45S mutant of annelid Arenicola marina peroxiredoxin 6 supports its assignment to the mechanistically typical 2-Cys subfamily without any formation of toroid-shaped decamers Smeets, Aude; Loumaye, Eléonore; Clippe, André Protein Science, Vol. 17, Issue 4 https://doi.org/10.1110/ps.073399308	journal	April 2008
Reduction of 1-Cys peroxiredoxins by ascorbate changes the thiol-specific antioxidant paradigm, revealing another function of vitamin C Monteiro, G.; Horta, B. B.; Pimenta, D. C. Proceedings of the National Academy of Sciences, Vol. 104, Issue 12 https://doi.org/10.1073/pnas.0700481104	journal	March 2007
Identification of a Human cDNA Clone for Lysosomal Type Ca ²⁺ -independent Phospholipase A ₂ and Properties of the Expressed Protein Kim, Tae-Suk; Sundaresh, Chennarayapatna S.; Feinstein, Sheldon I. Journal of Biological Chemistry, Vol. 272, Issue 4 https://doi.org/10.1074/jbc.272.4.2542	journal	January 1997
Crystal structure of decameric 2-Cys peroxiredoxin from human erythrocytes at 1.7Å resolution Schröder, Ewald; Littlechil*, Jennifer A.; Lebedev, Andrey A. Structure, Vol. 8, Issue 6 https://doi.org/10.1016/S0969-2126(00)00147-7	journal	June 2000
Serum Albumin Prevents Protein Aggregation and Amyloid Formation and Retains Chaperone-like Activity in the Presence of Physiological Ligands Finn, Thomas E.; Nunez, Andrea C.; Sunde, Margaret Journal of Biological Chemistry, Vol. 287, Issue 25 https://doi.org/10.1074/jbc.m112.372961	journal	May 2012
Scalable molecular dynamics with NAMD Phillips, James C.; Braun, Rosemary; Wang, Wei Journal of Computational Chemistry, Vol. 26, Issue 16, p. 1781-1802 https://doi.org/10.1002/jcc.20289	journal	January 2005
Moonlighting by Different Stressors: Crystal Structure of the Chaperone Species of a 2-Cys Peroxiredoxin Saccoccia, Fulvio; Di Micco, Patrizio; Boumis, Giovanna Structure, Vol. 20, Issue 3 https://doi.org/10.1016/j.str.2012.01.004	journal	March 2012
PREX: PeroxiRedoxin classification indEX, a database of subfamily assignments across the diverse peroxiredoxin family Soito, Laura; Williamson, Chris; Knutson, Stacy T. Nucleic Acids Research, Vol. 39, Issue suppl_1 https://doi.org/10.1093/nar/gkq1060	journal	October 2010
Numerical integration of the cartesian equations of motion of a system with constraints: molecular dynamics of n-alkanes Ryckaert, Jean-Paul; Ciccotti, Giovanni; Berendsen, Herman J. C. Journal of Computational Physics, Vol. 23, Issue 3 https://doi.org/10.1016/0021-9991(77)90098-5	journal	March 1977
On the evolutionary origin of the chaperonins Dekker, Carien; Willison, Keith R.; Taylor, William R. Proteins: Structure, Function, and Bioinformatics, Vol. 79, Issue 4 https://doi.org/10.1002/prot.22952	journal	February 2011
The paleobiological record of photosynthesis William Schopf, J. Photosynthesis Research, Vol. 107, Issue 1 https://doi.org/10.1007/s11120-010-9577-1	journal	July 2010
A Comprehensive Analysis of the Peroxiredoxin Reduction System in the Cyanobacterium Synechocystis sp. Strain PCC 6803 Reveals that All Five Peroxiredoxins Are Thioredoxin Dependent Perez-Perez, M. E.; Mata-Cabana, A.; Sanchez-Riego, A. M. Journal of Bacteriology, Vol. 191, Issue 24 https://doi.org/10.1128/JB.00831-09	journal	October 2009
How good are my data and what is the resolution? Evans, Philip R.; Murshudov, Garib N. Acta Crystallographica Section D Biological Crystallography, Vol. 69, Issue 7 https://doi.org/10.1107/S0907444913000061	journal	June 2013
Crystal Structure of Reduced and of Oxidized Peroxiredoxin IV Enzyme Reveals a Stable Oxidized Decamer and a Non-disulfide-bonded Intermediate in the Catalytic Cycle Cao, Zhenbo; Tavender, Timothy J.; Roszak, Aleksander W. Journal of Biological Chemistry, Vol. 286, Issue 49 https://doi.org/10.1074/jbc.m111.298810	journal	October 2011
AhpC (alkyl hydroperoxide reductase) from Anabaena sp. PCC 7120 protects Escherichia coli from multiple abiotic stresses Mishra, Yogesh; Chaurasia, Neha; Rai, Lal Chand Biochemical and Biophysical Research Communications, Vol. 381, Issue 4 https://doi.org/10.1016/j.bbrc.2009.02.100	journal	April 2009
Peroxiredoxin-1 from the Human Hookworm Ancylostoma ceylanicum Forms a Stable Oxidized Decamer and Is Covalently Inhibited by Conoidin A Nguyen, Jennifer B.; Pool, Christopher D.; Wong, Christina Y. B. Chemistry & Biology, Vol. 20, Issue 8 https://doi.org/10.1016/j.chembiol.2013.06.011	journal	August 2013
Modelling dynamics in protein crystal structures by ensemble refinement Burnley, B. Tom; Afonine, Pavel V.; Adams, Paul D. eLife, Vol. 1 https://doi.org/10.7554/eLife.00311	journal	December 2012
Joint X-ray and neutron refinement with phenix.refine Afonine, Pavel V.; Mustyakimov, Marat; Grosse-Kunstleve, Ralf W. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 11 https://doi.org/10.1107/S0907444910026582	journal	October 2010
Oxidation of archaeal peroxiredoxin involves a hypervalent sulfur intermediate Nakamura, T.; Yamamoto, T.; Abe, M. Proceedings of the National Academy of Sciences, Vol. 105, Issue 17 https://doi.org/10.1073/pnas.0709822105	journal	April 2008
Analysis of the peroxiredoxin family: Using active-site structure and sequence information for global classification and residue analysis Nelson, Kimberly J.; Knutson, Stacy T.; Soito, Laura Proteins: Structure, Function, and Bioinformatics, Vol. 79, Issue 3 https://doi.org/10.1002/prot.22936	journal	December 2010
Features and development of Coot Emsley, P.; Lohkamp, B.; Scott, W. G. Acta Crystallographica Section D Biological Crystallography, Vol. 66, Issue 4 https://doi.org/10.1107/S0907444910007493	journal	March 2010
Oxidative Stress-dependent Structural and Functional Switching of a Human 2-Cys Peroxiredoxin Isotype II That Enhances HeLa Cell Resistance to H ₂ O ₂ -induced Cell Death Moon, Jeong Chan; Hah, Young-Sool; Kim, Woe Yeon Journal of Biological Chemistry, Vol. 280, Issue 31 https://doi.org/10.1074/jbc.M505362200	journal	June 2005
ICM?A new method for protein modeling and design: Applications to docking and structure prediction from the distorted native conformation Abagyan, Ruben; Totrov, Maxim; Kuznetsov, Dmitry Journal of Computational Chemistry, Vol. 15, Issue 5 https://doi.org/10.1002/jcc.540150503	journal	May 1994
Chaperone-like features of bovine serum albumin: a comparison with α-crystallin Marini, I.; Moschini, R.; Corso, A. Del Cellular and Molecular Life Sciences, Vol. 62, Issue 24 https://doi.org/10.1007/s00018-005-5397-4	journal	November 2005
Catalase (KatA) and Alkyl Hydroperoxide Reductase (AhpC) Have Compensatory Roles in Peroxide Stress Resistance and Are Required for Survival, Persistence, and Nasal Colonization in Staphylococcus aureus Cosgrove, K.; Coutts, G.; Jonsson, I. -M. Journal of Bacteriology, Vol. 189, Issue 3 https://doi.org/10.1128/JB.01524-06	journal	November 2006
Characterization of a Mammalian Peroxiredoxin That Contains One Conserved Cysteine Kang, Sang Won; Baines, Ivan C.; Rhee, Sue Goo Journal of Biological Chemistry, Vol. 273, Issue 11 https://doi.org/10.1074/jbc.273.11.6303	journal	March 1998
Hydrogen peroxide production during experimental protein glycation Jiang, Zhen-Yue; Woollard, Alison C. S.; Wolff, Simon P. FEBS Letters, Vol. 268, Issue 1 https://doi.org/10.1016/0014-5793(90)80974-N	journal	July 1990
A smooth particle mesh Ewald method Essmann, Ulrich; Perera, Lalith; Berkowitz, Max L. The Journal of Chemical Physics, Vol. 103, Issue 19 https://doi.org/10.1063/1.470117	journal	November 1995
Receptor binding and priming of the spike protein of SARS-CoV-2 for membrane fusion Benton, Donald J.; Wrobel, Antoni G.; Xu, Pengqi Nature, Vol. 588, Issue 7837 https://doi.org/10.1038/s41586-020-2772-0	journal	September 2020
The isolation and purification of a specific “protector” protein which inhibits enzyme inactivation by a thiol/Fe(III)/O2 mixed-function oxidation system. Kim, K.; Kim, I. H.; Lee, K. Y. Journal of Biological Chemistry, Vol. 263, Issue 10 https://doi.org/10.1016/S0021-9258(18)68840-4	journal	April 1988
Turnover of the photosystem II D1 protein in higher plants under photoinhibitory and nonphotoinhibitory irradiance. Sundby, C.; McCaffery, S.; Anderson, J. M. Journal of Biological Chemistry, Vol. 268, Issue 34 https://doi.org/10.1016/S0021-9258(19)74416-0	journal	December 1993

Similar Records

Non-reductive modulation of chloroplast fructose-1,6-bisphosphatase by 2-Cys peroxiredoxin

Journal Article · Fri Apr 13 00:00:00 EDT 2007 · Biochemical and Biophysical Research Communications · OSTI ID:1490797

Caporaletti, Daniel; D'Alessio, Ana C; Rodriguez-Suarez, Roberto J; +3 more

Specific Glucoside Transporters Influence Septal Structure and Function in the Filamentous, Heterocyst-Forming Cyanobacterium Anabaena sp. Strain PCC 7120

Journal Article · Tue Jan 17 00:00:00 EST 2017 · Journal of Bacteriology · OSTI ID:1490797

Nieves-Morión, Mercedes; Lechno-Yossef, Sigal; López-Igual, Rocío; +6 more

Structure of the Sulphiredoxin-Peroxiredoxin Complex Reveals an Essential Repair Embrace

Journal Article · Tue Jan 01 00:00:00 EST 2008 · Nature · OSTI ID:1490797

Jonsson, T; Johnson, L; Lowther, W

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES

Title: Active-site plasticity revealed in the asymmetric dimer of AnPrx6 the 1-Cys peroxiredoxin and molecular chaperone from Anabaena sp. PCC 7120

Citation Formats

References (89)

Similar Records

Related Subjects