Megahertz serial crystallography

Wiedorn, Max O.; Oberthür, Dominik; Bean, Richard; Schubert, Robin; Werner, Nadine; Abbey, Brian; Aepfelbacher, Martin; Adriano, Luigi; Allahgholi, Aschkan; Al-Qudami, Nasser; Andreasson, Jakob; Aplin, Steve; Awel, Salah; Ayyer, Kartik; Bajt, Saša; Barák, Imrich; Bari, Sadia; Bielecki, Johan; Botha, Sabine; Boukhelef, Djelloul; Brehm, Wolfgang; Brockhauser, Sandor; Cheviakov, Igor; Coleman, Matthew A.; Cruz-Mazo, Francisco; Danilevski, Cyril; Darmanin, Connie; Doak, R. Bruce; Domaracky, Martin; Dörner, Katerina; Du, Yang; Fangohr, Hans; Fleckenstein, Holger; Frank, Matthias; Fromme, Petra; Gañán-Calvo, Alfonso M.; Gevorkov, Yaroslav; Giewekemeyer, Klaus; Ginn, Helen Mary; Graafsma, Heinz; Graceffa, Rita; Greiffenberg, Dominic; Gumprecht, Lars; Göttlicher, Peter; Hajdu, Janos; Hauf, Steffen; Heymann, Michael; Holmes, Susannah; Horke, Daniel A.; Hunter, Mark S.; Imlau, Siegfried; Kaukher, Alexander; Kim, Yoonhee; Klyuev, Alexander; Knoška, Juraj; Kobe, Bostjan; Kuhn, Manuela; Kupitz, Christopher; Küpper, Jochen; Lahey-Rudolph, Janine Mia; Laurus, Torsten; Le Cong, Karoline; Letrun, Romain; Xavier, P. Lourdu; Maia, Luis; Maia, Filipe R.  N.  C.; Mariani, Valerio; Messerschmidt, Marc; Metz, Markus; Mezza, Davide; Michelat, Thomas; Mills, Grant; Monteiro, Diana C.  F.; Morgan, Andrew; Mühlig, Kerstin; Munke, Anna; Münnich, Astrid; Nette, Julia; Nugent, Keith A.; Nuguid, Theresa; Orville, Allen M.; Pandey, Suraj; Pena, Gisel; Villanueva-Perez, Pablo; Poehlsen, Jennifer; Previtali, Gianpietro; Redecke, Lars; Riekehr, Winnie Maria; Rohde, Holger; Round, Adam; Safenreiter, Tatiana; Sarrou, Iosifina; Sato, Tokushi; Schmidt, Marius; Schmitt, Bernd; Schönherr, Robert; Schulz, Joachim; Sellberg, Jonas A.; Seibert, M. Marvin; Seuring, Carolin; Shelby, Megan L.; Shoeman, Robert L.; Sikorski, Marcin; Silenzi, Alessandro; Stan, Claudiu A.; Shi, Xintian; Stern, Stephan; Sztuk-Dambietz, Jola; Szuba, Janusz; Tolstikova, Aleksandra; Trebbin, Martin; Trunk, Ulrich; Vagovic, Patrik; Ve, Thomas; Weinhausen, Britta; White, Thomas A.; Wrona, Krzysztof; Xu, Chen; Yefanov, Oleksandr; Zatsepin, Nadia; Zhang, Jiaguo; Perbandt, Markus; Mancuso, Adrian P.; Betzel, Christian; Chapman, Henry; Barty, Anton

doi:10.1038/s41467-018-06156-7

Title: Megahertz serial crystallography

Journal Article · Tue Oct 02 00:00:00 EDT 2018 · Nature Communications

DOI:https://doi.org/10.1038/s41467-018-06156-7· OSTI ID:1490639

; Oberthür, Dominik; Bean, Richard; Schubert, Robin; Werner, Nadine;

; Aepfelbacher, Martin; Adriano, Luigi; Allahgholi, Aschkan; Al-Qudami, Nasser; Andreasson, Jakob; Aplin, Steve; Awel, Salah;

; Bajt, Saša;

;

; Bielecki, Johan; Botha, Sabine; Boukhelef, Djelloul more »

AbstractThe new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: USDOE National Nuclear Security Administration (NNSA)

Contributing Organization:: DESY

Grant/Contract Number:: AC02-76SF00515; AC52-07NA27344

OSTI ID:: 1490639

Alternate ID(s):: OSTI ID: 1959486

Report Number(s):: LLNL-JRNL-843452; PII: 6156

Journal Information:: Nature Communications, Vol. 9, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 113 works

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A versatile liquid-jet setup for the European XFEL Schulz, J.; Bielecki, J.; Doak, R. B. Journal of Synchrotron Radiation, Vol. 26, Issue 2 https://doi.org/10.1107/s1600577519000894	journal	February 2019
Double-pulse speckle contrast correlations with near Fourier transform limited free-electron laser light using hard split-and-delay Roseker, Wojciech; Lee, Sooheyong; Walther, Michael Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-01030	text	January 2020
3D printed nozzles on a silicon fluidic chip Bohne, Sven; Heymann, Michael; Chapman, Henry N. Review of Scientific Instruments, Vol. 90, Issue 3 https://doi.org/10.1063/1.5080428	journal	March 2019
A guide to sample delivery systems for serial crystallography Zhao, Feng‐Zhu; Zhang, Bin; Yan, Er‐Kai The FEBS Journal, Vol. 286, Issue 22 https://doi.org/10.1111/febs.15099	journal	August 2019
Time-resolved serial femtosecond crystallography at the European XFEL Pandey, Suraj; Bean, Richard; Sato, Tokushi Nature Methods https://doi.org/10.1038/s41592-019-0628-z	journal	November 2019
Megahertz single-particle imaging at the European XFEL Sobolev, Egor; Zolotarev, Sergei; Giewekemeyer, Klaus Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-02231	text	January 2020
Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14434-6	journal	January 2020
New Trends in Protein Crystallization and Protein Crystallography Moreno, Abel Crystals, Vol. 10, Issue 1 https://doi.org/10.3390/cryst10010046	journal	January 2020
Serial protein crystallography in an electron microscope Bücker, Robert; Hogan-Lamarre, Pascal; Mehrabi, Pedram Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-14793-0	journal	February 2020
Membrane protein megahertz crystallography at the European XFEL Gisriel, Chris; Coe, Jesse; Letrun, Romain Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-03909	text	January 2019
Ultracompact 3D microfluidics for time-resolved structural biology Knoška, Juraj; Adriano, Luigi; Awel, Salah Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00687	text	January 2020
MHz Data Collection of a Microcrystalline mMxture of Different Jack Bean Proteins Grünbein, Marie Luise; Bielecki, Johan; Gorel, Alexander Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2020-00843	text	January 2019
Spatial and temporal pre-alignment of an X-ray split-and-delay unit by laser light interferometry Roseker, Wojciech; Lee, S.; Walther, Michael Deutsches Elektronen-Synchrotron, DESY, Hamburg https://doi.org/10.3204/pubdb-2019-01681	text	January 2019