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Title: Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere

Abstract

Here, as part of a study investigating the rhizosphere and endosphere of the Eastern cottonwood tree, Populus deltoides, a number of isolates were subjected to genome sequencing. The genome-derived 16S rRNA gene sequence of strain CF314 T was 97.0 % similar to those of the Chryseobacterium daecheongense and Chryseobacterium polytrichastri type strains, but was essentially equidistant from many other Chryseobacterium type strains. Overall genome similarity metrics (average nucleotide identity, digital DNA–DNA hybridization, average amino acid identity) revealed greatest similarity to the Chryseobacterium daecheongense , Chryseobacterium piperi and Chryseobacterium soldanellicola type strains, but were well below the species thresholds. Strain CF314 T had a typical fatty acid composition for Chryseobacterium species and produced flexirubin pigments, but not carotenoids. The genome encodes a number of proteins such as a C-type lectin and terpene synthases that are also found in other plant-associated Bacteroidetes. Based on phenotypic and genomic characteristics of the strain, we propose the new species Chryseobacterium populi. Finally, the type strain is CF314 T=KCTC 62722 T=LMG 30786 T.

Authors:
 [1]; ORCiD logo [2];  [3]
  1. Lycoming College, Williamsport, PA (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Lycoming College, Williamsport, PA (United States); University of the Free State, Bloemfontein (South Africa)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1490595
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
International Journal of Systematic and Evolutionary Microbiology
Additional Journal Information:
Journal Volume: 69; Journal Issue: 2; Journal ID: ISSN 1466-5026
Publisher:
International Union of Microbiological Societies
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES; Chryseobacterium; populus deltoides; endosphere

Citation Formats

Bortniak, Victoria L., Pelletier, Dale A., and Newman, Jeffrey D. Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere. United States: N. p., 2018. Web. doi:10.1099/ijsem.0.003140.
Bortniak, Victoria L., Pelletier, Dale A., & Newman, Jeffrey D. Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere. United States. doi:10.1099/ijsem.0.003140.
Bortniak, Victoria L., Pelletier, Dale A., and Newman, Jeffrey D. Thu . "Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere". United States. doi:10.1099/ijsem.0.003140.
@article{osti_1490595,
title = {Chryseobacterium populi sp. nov., isolated from Populus deltoides endosphere},
author = {Bortniak, Victoria L. and Pelletier, Dale A. and Newman, Jeffrey D.},
abstractNote = {Here, as part of a study investigating the rhizosphere and endosphere of the Eastern cottonwood tree, Populus deltoides, a number of isolates were subjected to genome sequencing. The genome-derived 16S rRNA gene sequence of strain CF314T was 97.0 % similar to those of the Chryseobacterium daecheongense and Chryseobacterium polytrichastri type strains, but was essentially equidistant from many other Chryseobacterium type strains. Overall genome similarity metrics (average nucleotide identity, digital DNA–DNA hybridization, average amino acid identity) revealed greatest similarity to the Chryseobacterium daecheongense , Chryseobacterium piperi and Chryseobacterium soldanellicola type strains, but were well below the species thresholds. Strain CF314T had a typical fatty acid composition for Chryseobacterium species and produced flexirubin pigments, but not carotenoids. The genome encodes a number of proteins such as a C-type lectin and terpene synthases that are also found in other plant-associated Bacteroidetes. Based on phenotypic and genomic characteristics of the strain, we propose the new species Chryseobacterium populi. Finally, the type strain is CF314T=KCTC 62722T=LMG 30786T.},
doi = {10.1099/ijsem.0.003140},
journal = {International Journal of Systematic and Evolutionary Microbiology},
issn = {1466-5026},
number = 2,
volume = 69,
place = {United States},
year = {2018},
month = {12}
}

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The RAST Server: Rapid Annotations using Subsystems Technology
journal, January 2008

  • Aziz, Ramy K.; Bartels, Daniela; Best, Aaron A.
  • BMC Genomics, Vol. 9, Issue 1, Article No. 75
  • DOI: 10.1186/1471-2164-9-75