skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Abstract

Here, we developed a CRISPR/Cas9 and homology-directed-repair assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.

Authors:
ORCiD logo [1]; ORCiD logo [1]; ORCiD logo [1];  [2];  [1];  [1];  [3]; ORCiD logo [4]
  1. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  2. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Shanghai Jiao Tong Univ., Shanghai (China)
  3. Metabolic Engineering Research Lab. (Singapore)
  4. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States); Metabolic Engineering Research Lab. (Singapore)
Publication Date:
Research Org.:
Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23). Biological Systems Science Division
OSTI Identifier:
1489638
Grant/Contract Number:  
SC0018260
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Nature Biotechnology
Additional Journal Information:
Journal Volume: 36; Journal Issue: 6; Journal ID: ISSN 1087-0156
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, and Zhao, Huimin. Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. United States: N. p., 2018. Web. doi:10.1038/nbt.4132.
Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, & Zhao, Huimin. Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. United States. doi:10.1038/nbt.4132.
Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, and Zhao, Huimin. Mon . "Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision". United States. doi:10.1038/nbt.4132.
@article{osti_1489638,
title = {Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision},
author = {Bao, Zehua and HamediRad, Mohammad and Xue, Pu and Xiao, Han and Tasan, Ipek and Chao, Ran and Liang, Jing and Zhao, Huimin},
abstractNote = {Here, we developed a CRISPR/Cas9 and homology-directed-repair assisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.},
doi = {10.1038/nbt.4132},
journal = {Nature Biotechnology},
number = 6,
volume = 36,
place = {United States},
year = {Mon May 07 00:00:00 EDT 2018},
month = {Mon May 07 00:00:00 EDT 2018}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on May 7, 2019
Publisher's Version of Record

Citation Metrics:
Cited by: 7 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

Multiplex Genome Engineering Using CRISPR/Cas Systems
journal, January 2013


Rapid profiling of a microbial genome using mixtures of barcoded oligonucleotides
journal, July 2010

  • Warner, Joseph R.; Reeder, Philippa J.; Karimpour-Fard, Anis
  • Nature Biotechnology, Vol. 28, Issue 8, p. 856-862
  • DOI: 10.1038/nbt.1653

A guide to genome engineering with programmable nucleases
journal, April 2014

  • Kim, Hyongbum; Kim, Jin-Soo
  • Nature Reviews Genetics, Vol. 15, Issue 5, p. 321-334
  • DOI: 10.1038/nrg3686

Strategy for directing combinatorial genome engineering in Escherichia coli
journal, June 2012

  • Sandoval, N. R.; Kim, J. Y. H.; Glebes, T. Y.
  • Proceedings of the National Academy of Sciences, Vol. 109, Issue 26, p. 10540-10545
  • DOI: 10.1073/pnas.1206299109

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering
journal, December 2016

  • Garst, Andrew D.; Bassalo, Marcelo C.; Pines, Gur
  • Nature Biotechnology, Vol. 35, Issue 1, p. 48-55
  • DOI: 10.1038/nbt.3718

Programming cells by multiplex genome engineering and accelerated evolution
journal, July 2009

  • Wang, Harris H.; Isaacs, Farren J.; Carr, Peter A.
  • Nature, Vol. 460, Issue 7257, p. 894-898
  • DOI: 10.1038/nature08187