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Title: Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440

Abstract

Nitrogen supply is critical to the synthesis of intracellular PHA in various bacteria. However, the specific role of the nitrogen in synthesizing PHA from benzoate, a lignin model compound use for the study of bacteria catabolism of aromatics, is still not clear. Here, two culture conditions were maintained for Pseudomonas putida KT2440 to produce PHA using benzoate as a carbon source. Under nitrogen-limited and surplus conditions, the accumulation of PHA was to 37.3% and 0.25% of cell dry weight, respectively. A model fit to the kinetics of biomass growth and PHA accumulation showed good agreement with data. GC–MS and NMR showed that PHA contained six hydroxyl fatty acid monomers under nitrogen-limited conditions, while two monomers were identified under nitrogen surplus conditions. The average molecular weight of PHA increased after the nitrogen source was exhausted. Lastly, these results provide a promising strategy for optimization of lignin to PHA yields.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1];  [1]; ORCiD logo [3];  [4];  [1]
  1. Washington State Univ., Richland, WA (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States)
  3. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Texas A & M Univ., College Station, TX (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1489561
Grant/Contract Number:  
AC05-00OR22725; EE0007104; EE0006112
Resource Type:
Journal Article: Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 273; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Polyhydroxyalkanoates; Pseudomonas putida KT2440; Benzoate; Nitrogen; Kinetic modeling

Citation Formats

Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, and Yang, Bin. Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440. United States: N. p., 2018. Web. doi:10.1016/j.biortech.2018.11.046.
Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, & Yang, Bin. Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440. United States. doi:10.1016/j.biortech.2018.11.046.
Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, and Yang, Bin. Mon . "Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440". United States. doi:10.1016/j.biortech.2018.11.046.
@article{osti_1489561,
title = {Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440},
author = {Xu, Zhangyang and Li, Xiaolu and Hao, Naijia and Pan, Chummei and de la torre, Luis and Ahamed, Aftab and Miller, John H. and Ragauskas, Arthur J. and Yuan, Joshua and Yang, Bin},
abstractNote = {Nitrogen supply is critical to the synthesis of intracellular PHA in various bacteria. However, the specific role of the nitrogen in synthesizing PHA from benzoate, a lignin model compound use for the study of bacteria catabolism of aromatics, is still not clear. Here, two culture conditions were maintained for Pseudomonas putida KT2440 to produce PHA using benzoate as a carbon source. Under nitrogen-limited and surplus conditions, the accumulation of PHA was to 37.3% and 0.25% of cell dry weight, respectively. A model fit to the kinetics of biomass growth and PHA accumulation showed good agreement with data. GC–MS and NMR showed that PHA contained six hydroxyl fatty acid monomers under nitrogen-limited conditions, while two monomers were identified under nitrogen surplus conditions. The average molecular weight of PHA increased after the nitrogen source was exhausted. Lastly, these results provide a promising strategy for optimization of lignin to PHA yields.},
doi = {10.1016/j.biortech.2018.11.046},
journal = {Bioresource Technology},
issn = {0960-8524},
number = C,
volume = 273,
place = {United States},
year = {2018},
month = {11}
}

Journal Article:
Free Publicly Available Full Text
This content will become publicly available on November 12, 2019
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