skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Abstract

We developed a CRISPR–Cas9- and homology-directed-repairassisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.

Authors:
ORCiD logo; ORCiD logo; ORCiD logo; ; ; ; ; ORCiD logo
Publication Date:
Research Org.:
University of Illinois at Urbana-Champaign
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1486877
DOE Contract Number:  
SC0018260
Resource Type:
Journal Article
Resource Relation:
Journal Name: Nature Biotechnology; Journal Volume: 36; Journal Issue: 6
Country of Publication:
United States
Language:
English

Citation Formats

Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, and Zhao, Huimin. Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. United States: N. p., 2018. Web. doi:10.1038/nbt.4132.
Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, & Zhao, Huimin. Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision. United States. doi:10.1038/nbt.4132.
Bao, Zehua, HamediRad, Mohammad, Xue, Pu, Xiao, Han, Tasan, Ipek, Chao, Ran, Liang, Jing, and Zhao, Huimin. Mon . "Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision". United States. doi:10.1038/nbt.4132.
@article{osti_1486877,
title = {Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision},
author = {Bao, Zehua and HamediRad, Mohammad and Xue, Pu and Xiao, Han and Tasan, Ipek and Chao, Ran and Liang, Jing and Zhao, Huimin},
abstractNote = {We developed a CRISPR–Cas9- and homology-directed-repairassisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.},
doi = {10.1038/nbt.4132},
journal = {Nature Biotechnology},
number = 6,
volume = 36,
place = {United States},
year = {Mon May 07 00:00:00 EDT 2018},
month = {Mon May 07 00:00:00 EDT 2018}
}