Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Bao, Zehua; HamediRad, Mohammad; Xue, Pu; Xiao, Han; Tasan, Ipek; Chao, Ran; Liang, Jing; Zhao, Huimin

doi:10.1038/nbt.4132

Genome-scale engineering of Saccharomyces cerevisiae with single-nucleotide precision

Journal Article · 07 May 2018 · Nature Biotechnology

DOI:https://doi.org/10.1038/nbt.4132· OSTI ID:1486877

; ; ; Xiao, Han; Tasan, Ipek; Chao, Ran; Liang, Jing;

We developed a CRISPR–Cas9- and homology-directed-repairassisted genome-scale engineering method named CHAnGE that can rapidly output tens of thousands of specific genetic variants in yeast. More than 98% of target sequences were efficiently edited with an average frequency of 82%. We validate the single-nucleotide resolution genome-editing capability of this technology by creating a genome-wide gene disruption collection and apply our method to improve tolerance to growth inhibitors.

Research Organization:: Univ. of Illinois at Urbana-Champaign, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0018260

OSTI ID:: 1486877

Journal Information:: Nature Biotechnology, Vol. 36, Issue 6; ISSN 1087-0156

Publisher:: Springer Nature

Country of Publication:: United States

Language:: English

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