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Title: An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China

Abstract

Despite its importance, anammox (anaerobic ammonium oxidation) in estuarine sediment systems remains poorly understood, particularly at the continental scale. This study aimed to understand the abundance, diversity, and activity of anammox bacteria and to determine the main factors influencing the anammox process in estuarine sediments in China. Materials and methods Estuarine sediments were collected from 18 estuaries spanning over 4000 km. Experiments using an (15)Ntracer, quantitative PCR, and clone library construction were used to determine the activity, abundance, and diversity of anammox bacteria. The impact of environmental factors on anammox processes was also determined. Results and discussion The abundance of the anammox-specific hydrazine synthase (hzsB) gene ranged from 1.8 x 105 +/- 3.4 x 104 to 3.6 x 108 +/- 7.5 x 107 copies g(-1) dw. Candidatus Scalindua, Brocadia, Kuenenia, Jettenia, and two novel unidentified clusters were detected, with Scalindua dominating the anammox population. Additionally, the abundances of Scalindua, Kuenenia, and Brocadia were found to be significantly correlated with latitude. The anammox rates ranged from 0.29 +/- 0.15 to 13.68 +/- 3.98 nmol N g(-1) dw h(-1) and contributed to 2.39-82.61% of total N-2 production. Pearson correlation analysis revealed that the anammox rate was positively correlated with total nitrogen, totalmore » carbon, and temperature, and was negatively correlated with dissolved oxygen (DO). The key factors influencing the hzsB gene abundance were ammonium concentration, salinity, and DO. Ammonium concentration, pH, temperature, and latitude were main variables shaping the anammox-associated bacterial community. Conclusions Our results suggested that anammox bacteria are ubiquitous in coastal estuaries in China and underline the importance of anammox resulting in N loss at a continental scale.« less

Authors:
; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Natural Science Foundation of China (NNSFC); China Postdoctoral Science Foundation; Ministry of Science and Technology of the People's Republic of China - International Science and Technology Cooperation Program
OSTI Identifier:
1484486
DOE Contract Number:  
AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Journal of Soils and Sediments
Additional Journal Information:
Journal Volume: 17; Journal Issue: 10; Journal ID: ISSN 1439-0108
Country of Publication:
United States
Language:
English
Subject:
N loss; activity; anammox; estuarine sediments; spatial variation

Citation Formats

Yang, Xiao-Ru, Weng, Bo-Sen, Li, Hu, Marshall, Christopher W., Li, Hong, Chen, Yong-Shan, Yu, Shen, Zhu, Gui-Bing, and Zhu, Yong-Guan. An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China. United States: N. p., 2017. Web. doi:10.1007/s11368-017-1728-y.
Yang, Xiao-Ru, Weng, Bo-Sen, Li, Hu, Marshall, Christopher W., Li, Hong, Chen, Yong-Shan, Yu, Shen, Zhu, Gui-Bing, & Zhu, Yong-Guan. An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China. United States. doi:10.1007/s11368-017-1728-y.
Yang, Xiao-Ru, Weng, Bo-Sen, Li, Hu, Marshall, Christopher W., Li, Hong, Chen, Yong-Shan, Yu, Shen, Zhu, Gui-Bing, and Zhu, Yong-Guan. Tue . "An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China". United States. doi:10.1007/s11368-017-1728-y.
@article{osti_1484486,
title = {An overlooked nitrogen loss linked to anaerobic ammonium oxidation in estuarine sediments in China},
author = {Yang, Xiao-Ru and Weng, Bo-Sen and Li, Hu and Marshall, Christopher W. and Li, Hong and Chen, Yong-Shan and Yu, Shen and Zhu, Gui-Bing and Zhu, Yong-Guan},
abstractNote = {Despite its importance, anammox (anaerobic ammonium oxidation) in estuarine sediment systems remains poorly understood, particularly at the continental scale. This study aimed to understand the abundance, diversity, and activity of anammox bacteria and to determine the main factors influencing the anammox process in estuarine sediments in China. Materials and methods Estuarine sediments were collected from 18 estuaries spanning over 4000 km. Experiments using an (15)Ntracer, quantitative PCR, and clone library construction were used to determine the activity, abundance, and diversity of anammox bacteria. The impact of environmental factors on anammox processes was also determined. Results and discussion The abundance of the anammox-specific hydrazine synthase (hzsB) gene ranged from 1.8 x 105 +/- 3.4 x 104 to 3.6 x 108 +/- 7.5 x 107 copies g(-1) dw. Candidatus Scalindua, Brocadia, Kuenenia, Jettenia, and two novel unidentified clusters were detected, with Scalindua dominating the anammox population. Additionally, the abundances of Scalindua, Kuenenia, and Brocadia were found to be significantly correlated with latitude. The anammox rates ranged from 0.29 +/- 0.15 to 13.68 +/- 3.98 nmol N g(-1) dw h(-1) and contributed to 2.39-82.61% of total N-2 production. Pearson correlation analysis revealed that the anammox rate was positively correlated with total nitrogen, total carbon, and temperature, and was negatively correlated with dissolved oxygen (DO). The key factors influencing the hzsB gene abundance were ammonium concentration, salinity, and DO. Ammonium concentration, pH, temperature, and latitude were main variables shaping the anammox-associated bacterial community. Conclusions Our results suggested that anammox bacteria are ubiquitous in coastal estuaries in China and underline the importance of anammox resulting in N loss at a continental scale.},
doi = {10.1007/s11368-017-1728-y},
journal = {Journal of Soils and Sediments},
issn = {1439-0108},
number = 10,
volume = 17,
place = {United States},
year = {2017},
month = {5}
}

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