Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli

Liang, Liya; Heveran, Chelsea; Liu, Rongming; Gill, Ryan T.; Nagarajan, Aparna; Cameron, Jeffrey; Hubler, Mija; Srubar, Wil V.; Cook, Sherri M.

doi:10.1021/acssynbio.8b00194

Title: Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli

Journal Article · Fri Nov 02 00:00:00 EDT 2018 · ACS Synthetic Biology

DOI:https://doi.org/10.1021/acssynbio.8b00194· OSTI ID:1484428

Liang, Liya ^[1]; Heveran, Chelsea ^[2]; Liu, Rongming ^[1];

^[3]; Nagarajan, Aparna ^[1]; Cameron, Jeffrey ^[4]; Hubler, Mija ^[2]; Srubar, Wil V. ^[2];

^[2]

Renewable and Sustainable Energy Inst. (RASEI), Boulder, CO (United States)
Univ. of Colorado, Boulder, CO (United States). Dept. of Civil, Environmental, and Architectural Engineering
Renewable and Sustainable Energy Inst. (RASEI), Boulder, CO (United States); Univ. of Colorado, Boulder, CO (United States). Dept. of Chemical and Biological Engineering
Renewable and Sustainable Energy Inst. (RASEI), Boulder, CO (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States); Univ. of Colorado, Boulder, CO (United States). Dept. of Chemistry and Biochemistry

Ureolytic bacteria (e.g., Sporosarcina pasteurii) can produce calcium carbonate (CaCO₃). Tailoring the size and shape of biogenic CaCO₃ may increase the range of useful applications for these crystals. However, wild type Sporosarcina pasteurii is difficult to genetically engineer, limiting control of the organism and its crystal precipitates. Therefore, we designed, constructed, and compared different urease operons and expression levels for CaCO₃ production in engineered Escherichia coli strains. We quantified urease expression and calcium uptake and characterized CaCO₃ crystal phase and morphology for 13 engineered strains. There was a weak relationship between urease expression and crystal size, suggesting that genes surrounding the urease gene cluster affect crystal size. However, when evaluating strains with a wider range of urease expression levels, there was a negative relationship between urease activity and polycrystal size (e.g., larger crystals with lower activity). The resulting range of crystal morphologies created by the rationally designed strains demonstrates the potential for controlling biogenic CaCO₃ precipitation.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: U.S. Department of Defense (DOD), Defense Advanced Research Projects Agency

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1484428

Report Number(s):: NREL/JA-2700-72870

Journal Information:: ACS Synthetic Biology, Vol. 7, Issue 11; ISSN 2161-5063

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 13 works

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Metal and metalloid immobilization by microbiologically induced carbonates precipitation Tamayo-Figueroa, Diana P.; Castillo, Elianna; Brandão, Pedro F. B. World Journal of Microbiology and Biotechnology, Vol. 35, Issue 4 https://doi.org/10.1007/s11274-019-2626-9	journal	March 2019
Engineered Ureolytic Microorganisms Can Tailor the Morphology and Nanomechanical Properties of Microbial-Precipitated Calcium Carbonate Heveran, Chelsea M.; Liang, Liya; Nagarajan, Aparna Scientific Reports, Vol. 9, Issue 1 https://doi.org/10.1038/s41598-019-51133-9	journal	October 2019

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59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
CaCO3 precipitation
Escherichia coli
MICP
rational control
urease

Title: Rational Control of Calcium Carbonate Precipitation by Engineered Escherichia coli

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