Variant Enzymes

Foukaraki, Maria; Hommes, Ronaldus Wilhelmus; Kaper, Thijs; Kelemen, Bradley R.; Kralj, Slavko; Lantz, Suzanne E.; Nikolaev, Igor; Van Der Kley, Wilhelmus; Van Lieshout, Johannes Franciscus Thomas; Thans, Sander Van Stigt

Variant Enzymes

Patent · 25 September 2018

OSTI ID:1482204

Foukaraki, Maria; Hommes, Ronaldus Wilhelmus; Kaper, Thijs; Kelemen, Bradley R.; Kralj, Slavko; Lantz, Suzanne E.; Nikolaev, Igor; Van Der Kley, Wilhelmus; Van Lieshout, Johannes Franciscus Thomas; Thans, Sander Van Stigt

Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.

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Research Organization:: DANISCO US INC., Palo Alto, CA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC36-08GO18078

Assignee:: DANISCO US INC. (Palo Alto, CA)

Patent Number(s):: 10,081,802

Application Number:: 14/909,007

OSTI ID:: 1482204

Country of Publication:: United States

Language:: English

References (142)

Structural and functional characterization of a small chitin-active lytic polysaccharide monooxygenase domain of a multi-modular chitinase from Jonesia denitrificans Mekasha, Sophanit; Forsberg, Zarah; Dalhus, Bjørn FEBS Letters, Vol. 590, Issue 1, p. 34-42 https://doi.org/10.1002/1873-3468.12025	journal	December 2015
Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger. Boel, E.; Hansen, M. T.; Hjort, I. The EMBO Journal, Vol. 3, Issue 7, p. 1581-1585 https://doi.org/10.1002/j.1460-2075.1984.tb02014.x	journal	July 1984
Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose. Tormo, J.; Lamed, R.; Chirino, A. J. The EMBO Journal, Vol. 15, Issue 21, p. 5739-5751 https://doi.org/10.1002/j.1460-2075.1996.tb00960.x	journal	November 1996
Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae Panttilä, Merja E.; André, Lars; Saloheimo, Markku Yeast, Vol. 3, Issue 3, p. 175-185 https://doi.org/10.1002/yea.320030305	journal	September 1987
Activity Studies and Crystal Structures of Catalytically Deficient Mutants of Cellobiohydrolase I fromTrichoderma reesei Ståhlberg, Jerry; Divne, Christina; Koivula, Anu Journal of Molecular Biology, Vol. 264, Issue 2 https://doi.org/10.1006/jmbi.1996.0644	journal	November 1996
Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor Schell, Daniel J.; Farmer, Jody; Newman, Millie Biotechnology for Fuels and Chemicals https://doi.org/10.1007/978-1-4612-0057-4_6	book	January 2003
Molecular Biology of Cellulolytic Fungi Nevalainen, H.; Penttilä, M.; Kück, Ulrich Genetics and Biotechnology, p. 303-319 https://doi.org/10.1007/978-3-662-10364-7_18	book	January 1995
Viruses and gene silencing in plants Baulcombe, D.; Calisher, Charles H.; Horzinek, M. C. 100 Years of Virology, p. 189-201 https://doi.org/10.1007/978-3-7091-6425-9_14	book	January 1999
Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins Ward, Michael; Wilson, Lori J.; Kodama, Katherine H. Applied Microbiology and Biotechnology, Vol. 39, Issue 6, p. 738-743 https://doi.org/10.1007/BF00164459	journal	August 1993
Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations Sheir-Neiss, G.; Montenecourt, B. S. Applied Microbiology and Biotechnology, Vol. 20, Issue 1, p. 46-53 https://doi.org/10.1007/BF00254645	journal	July 1984
Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae Sakamoto, S.; Tamura, G.; Ito, K. Current Genetics, Vol. 27, Issue 5, p. 435-439 https://doi.org/10.1007/BF00311212	journal	April 1995
Transformation of Trichoderma harzianum by high-voltage electric pulse Goldman, G. H.; Van Montagu, M.; Herrera-Estrella, A. Current Genetics, Vol. 17, Issue 2, p. 169-174 https://doi.org/10.1007/BF00312863	journal	February 1990
Primary structure of the trpC gene from Aspergillus nidulans Mullaney, Edward J.; Hamer, John E.; Roberti, Kellee A. MGG Molecular & General Genetics, Vol. 199, Issue 1, p. 37-45 https://doi.org/10.1007/BF00327506	journal	January 1985
Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA Lorito, M.; Hayes, C. K.; Di Pietro, A. Current Genetics, Vol. 24, Issue 4, p. 349-356 https://doi.org/10.1007/BF00336788	journal	October 1993
Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase Campbell, Edward I.; Unkles, Shiela E.; Macro, Janet A. Current Genetics, Vol. 16, Issue 1, p. 53-56 https://doi.org/10.1007/BF00411084	journal	July 1989
Characterization of cellulases by HPLC separation Fliess, A.; Schügerl, K. European Journal of Applied Microbiology and Biotechnology, Vol. 17, Issue 5, p. 314-318 https://doi.org/10.1007/BF00508028	journal	January 1983
Purification and properties of an extracellular β-glucosidase from Lenzites trabea Herr, D.; Baumer, F.; Dellweg, H. European Journal of Applied Microbiology and Biotechnology, Vol. 5, Issue 1, p. 29-36 https://doi.org/10.1007/BF00515684	journal	January 1978
Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12 Halldórsdóttir, S.; Thórólfsdót, E. T.; Spilliaert, R. Applied Microbiology and Biotechnology, Vol. 49, Issue 3, p. 277-284 https://doi.org/10.1007/s002530051169	journal	March 1998
Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase Goedegebuur, Frits; Fowler, Timothy; Phillips, Jay Current Genetics, Vol. 41, Issue 2, p. 89-98 https://doi.org/10.1007/s00294-002-0290-2	journal	May 2002
Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae Cummings, C.; Fowler, T. Current Genetics, Vol. 29, Issue 3 https://doi.org/10.1007/s002940050040	journal	February 1996
Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing Tanghe, Magali; Danneels, Barbara; Camattari, Andrea Molecular Biotechnology, Vol. 57, Issue 11-12, p. 1010-1017 https://doi.org/10.1007/s12033-015-9887-9	journal	August 2015
Quantitative interpretations of double mutations of enzymes Mildvan, Albert S.; Weber, David J.; Kuliopulos, Athan Archives of Biochemistry and Biophysics, Vol. 294, Issue 2, p. 327-340 https://doi.org/10.1016/0003-9861(92)90692-P	journal	May 1992
Enzymic activities of endo-1,4-β-d-glucanases purified from Trichoderma viride Shoemaker, S. P.; Brown, R. D. Biochimica et Biophysica Acta (BBA) - Enzymology, Vol. 523, Issue 1, p. 133-146 https://doi.org/10.1016/0005-2744(78)90016-5	journal	March 1978
Separation of endo- and exo-type cellulases using a new affinity chromatography method van Tilbeurgh, Herman; Bhikhabhai, Ramagauri; Göran Pettersson, L. FEBS Letters, Vol. 169, Issue 2, p. 215-218 https://doi.org/10.1016/0014-5793(84)80321-X	journal	April 1984
Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains Van Tilbeurgh, Herman; Tomme, Peter; Claeyssens, Marc FEBS Letters, Vol. 204, Issue 2, p. 223-227 https://doi.org/10.1016/0014-5793(86)80816-X	journal	August 1986
A general method applicable to the search for similarities in the amino acid sequence of two proteins Needleman, Saul B.; Wunsch, Christian D. Journal of Molecular Biology, Vol. 48, Issue 3, p. 443-453 https://doi.org/10.1016/0022-2836(70)90057-4	journal	March 1970
Methods for measuring cellulase activities Wood, Thomas M.; Bhat, K. Mahalingeshwara Methods in Enzymology, Vol. 160, p. 87-112 https://doi.org/10.1016/0076-6879(88)60109-1	journal	January 1988
Cellulases of Trichoderma reesei Schülein, Martin Methods in Enzymology, Vol. 160, p. 234-242 https://doi.org/10.1016/0076-6879(88)60125-X	journal	January 1988
[57] Rethinking your purification procedure Deutscher, Murray P. Guide to Protein Purification https://doi.org/10.1016/0076-6879(90)82059-B	book	January 1990
Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans Timberlake, William E.; Barnard, Elizabeth C. Cell, Vol. 26, Issue 1, p. 29-37 https://doi.org/10.1016/0092-8674(81)90030-1	journal	October 1981
Nucleotide sequence of the triosephosphate isomerase gene from Aspergillus nidulans: Implications for a differential loss of introns McKnight, Gary L.; O'Hara, Patrick J.; Parker, Michael L. Cell, Vol. 46, Issue 1, p. 143-147 https://doi.org/10.1016/0092-8674(86)90868-8	journal	July 1986
Purification and characterization of two extracellular β-glucosidases from Trichoderma reesei Chen, Huizhong; Hayn, Marianne; Esterbauer, Hermann Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1121, Issue 1-2, p. 54-60 https://doi.org/10.1016/0167-4838(92)90336-C	journal	May 1992
Cellulase families and their genes Knowles, Jonathan; Lehtovaara, Päivi; Teeri, Tuula Trends in Biotechnology, Vol. 5, Issue 9, p. 255-261 https://doi.org/10.1016/0167-7799(87)90102-8	journal	September 1987
Comparison of biosequences Smith, Temple F.; Waterman, Michael S. Advances in Applied Mathematics, Vol. 2, Issue 4, p. 482-489 https://doi.org/10.1016/0196-8858(81)90046-4	journal	December 1981
Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans Lockington, Robin A.; Scaly-Lewis, H. M.; Scazzocchio, C. Gene, Vol. 33, Issue 2, p. 137-149 https://doi.org/10.1016/0378-1119(85)90088-5	journal	January 1985
Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites Wells, James A.; Vasser, Mark; Powers, David B. Gene, Vol. 34, Issue 2-3, p. 315-323 https://doi.org/10.1016/0378-1119(85)90140-4	journal	January 1985
A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei Penttilä, Merja; Nevalainen, Helena; Rättö, Marjaana Gene, Vol. 61, Issue 2, p. 155-164 https://doi.org/10.1016/0378-1119(87)90110-7	journal	January 1987
Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae Rothstein, Steven J.; Lahners, Kristine N.; Lazarus, Colin M. Gene, Vol. 55, Issue 2-3, p. 353-356 https://doi.org/10.1016/0378-1119(87)90296-4	journal	January 1987
EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme Saloheimo, M.; Lehtovaara, P.; Penttilä, M. Gene, Vol. 63, Issue 1, p. 11-21 https://doi.org/10.1016/0378-1119(88)90541-0	journal	March 1988
Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae Penttilä, Merja E.; André, Lars; Lehtovaara, Päivi Gene, Vol. 63, Issue 1, p. 103-112 https://doi.org/10.1016/0378-1119(88)90549-5	journal	March 1988
CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora Saarilahti, Hannu T.; Henrissat, Bernard; Tapio Palva, E. Gene, Vol. 90, Issue 1, p. 9-14 https://doi.org/10.1016/0378-1119(90)90433-R	journal	May 1990
Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus Kawaguchi, Takashi; Enoki, Tatsuji; Tsurumaki, Shinji Gene, Vol. 173, Issue 2, p. 287-288 https://doi.org/10.1016/0378-1119(96)00179-5	journal	September 1996
A radiation hybrid map of 18 growth factor, growth factor receptor, hormone receptor, or neurotransmitter receptor genes on the distal region of the long arm of chromosome 5 Warrington, J. A.; Bailey, S. K.; Armstrong, E. Genomics, Vol. 13, Issue 3, p. 803-808 https://doi.org/10.1016/0888-7543(92)90156-M	journal	July 1992
Production of fully human antibodies by transgenic mice Jakobovits, Aya Current Opinion in Biotechnology, Vol. 6, Issue 5, p. 561-566 https://doi.org/10.1016/0958-1669(95)80093-X	journal	January 1995
Characteristics of fungal cellulases Goyal, Anil; Ghosh, Bijan; Eveleigh, Douglas Bioresource Technology, Vol. 36, Issue 1, p. 37-50 https://doi.org/10.1016/0960-8524(91)90098-5	journal	January 1991
Heterologous Gene Expression in Filamentous Fungi Van Den Hondel, Cees A. M. J. J.; Punt, Peter J.; Van Gorcom, Robert F. M. More Gene Manipulations in Fungi, p. 396-428 https://doi.org/10.1016/B978-0-12-088642-5.50025-9	book	December 1990
Recombinant PCR Higuchi, Russell PCR Protocols, p. 177-183 https://doi.org/10.1016/B978-0-12-372180-8.50026-3	book	December 1989
Chapter One - Heterologous Gene Expression in Filamentous Fungi Su, Xiaoyun; Schmitz, George; Zhang, Meiling Advances in Applied Microbiology, Vol. 81, p. 1-61 https://doi.org/10.1016/B978-0-12-394382-8.00001-0	book	January 2012
Genetically engineered peptide fusions for improved protein partitioning in aqueous two-phase systems Collén, Anna; Ward, Michael; Tjerneld, Folke Journal of Chromatography A, Vol. 910, Issue 2, p. 275-284 https://doi.org/10.1016/S0021-9673(00)01212-7	journal	March 2001
Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography Ellouz, S.; Durand, H.; Tiraby, G. Journal of Chromatography A, Vol. 396, p. 307-317 https://doi.org/10.1016/S0021-9673(01)94068-3	journal	January 1987
Ion-exchange chromatographic purification and quantitative analysis of Trichoderma reesei cellulases cellobiohydrolase I, II and endoglucanase II by fast protein liquid chromatography Medve, József; Lee, Dora; Tjerneld, Folke Journal of Chromatography A, Vol. 808, Issue 1-2, p. 153-165 https://doi.org/10.1016/S0021-9673(98)00132-0	journal	May 1998
Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction Tomaz, Cândida T.; Queiroz, João A. Journal of Chromatography A, Vol. 865, Issue 1-2, p. 123-128 https://doi.org/10.1016/S0021-9673(99)00851-1	journal	December 1999
SCOP: A structural classification of proteins database for the investigation of sequences and structures Murzin, Alexey G.; Brenner, Steven E.; Hubbard, Tim Journal of Molecular Biology, Vol. 247, Issue 4 https://doi.org/10.1016/S0022-2836(05)80134-2	journal	April 1995
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
[79] Purification of all glycolytic enzymes from one muscle extract Scopes, Robert K.; Stoter, Ann Methods in Enzymology, Vol. 90, p. 479-490 https://doi.org/10.1016/S0076-6879(82)90175-6	journal	January 1982
The roles and function of cellulose-binding domains Linder, Markus; Teeri, Tuula T. Journal of Biotechnology, Vol. 57, Issue 1-3, p. 15-28 https://doi.org/10.1016/S0168-1656(97)00087-4	journal	September 1997
Directed immobilization of recombinant staphylococci on cotton fibers by functional display of a fungal cellulose-binding domain Lehtiö, J. FEMS Microbiology Letters, Vol. 195, Issue 2 https://doi.org/10.1016/S0378-1097(00)00562-0	journal	February 2001
Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast Hari Krishna, S.; Janardhan Reddy, T.; Chowdary, G. V. Bioresource Technology, Vol. 77, Issue 2, p. 193-196 https://doi.org/10.1016/S0960-8524(00)00151-6	journal	April 2001
Kinetics of cellulose saccharification by Trichoderma reesei cellulases Ortega, N. International Biodeterioration & Biodegradation, Vol. 47, Issue 1, p. 7-14 https://doi.org/10.1016/S0964-8305(00)00101-3	journal	January 2001
A comparison of gel-based, nylon filter and microarray techniques to detect differential RNA expression in plants Baldwin, Don; Crane, Virginia; Rice, Douglas Current Opinion in Plant Biology, Vol. 2, Issue 2, p. 96-103 https://doi.org/10.1016/S1369-5266(99)80020-X	journal	April 1999
Site saturation mutagenesis: Methods and applications in protein engineering Siloto, Rodrigo M. P.; Weselake, Randall J. Biocatalysis and Agricultural Biotechnology, Vol. 1, Issue 3, p. 181-189 https://doi.org/10.1016/j.bcab.2012.03.010	journal	July 2012
Molecular phylogeny and species delimitation in the section Longibrachiatum of Trichoderma Druzhinina, Irina S.; Komoń-Zelazowska, Monika; Ismaiel, Adnan Fungal Genetics and Biology, Vol. 49, Issue 5, p. 358-368 https://doi.org/10.1016/j.fgb.2012.02.004	journal	May 2012
The First Structure of a Glycoside Hydrolase Family 61 Member, Cel61B from Hypocrea jecorina, at 1.6 Å Resolution Karkehabadi, Saeid; Hansson, Henrik; Kim, Steve Journal of Molecular Biology, Vol. 383, Issue 1, p. 144-154 https://doi.org/10.1016/j.jmb.2008.08.016	journal	October 2008
Characterization of the Chitinolytic Machinery of Enterococcus faecalis V583 and High-Resolution Structure of Its Oxidative CBM33 Enzyme Vaaje-Kolstad, Gustav; Bøhle, Liv Anette; Gåseidnes, Sigrid Journal of Molecular Biology, Vol. 416, Issue 2 https://doi.org/10.1016/j.jmb.2011.12.033	journal	February 2012
Microbial diversity of cellulose hydrolysis Wilson, David B. Current Opinion in Microbiology, Vol. 14, Issue 3 https://doi.org/10.1016/j.mib.2011.04.004	journal	June 2011
Structural diversity of lytic polysaccharide monooxygenases Vaaje-Kolstad, Gustav; Forsberg, Zarah; Loose, Jennifer SM Current Opinion in Structural Biology, Vol. 44 https://doi.org/10.1016/j.sbi.2016.12.012	journal	June 2017
Structural Basis for Substrate Targeting and Catalysis by Fungal Polysaccharide Monooxygenases Li, Xin; Beeson, William T.; Phillips, Christopher M. Structure, Vol. 20, Issue 6, p. 1051-1061 https://doi.org/10.1016/j.str.2012.04.002	journal	June 2012
Inverse Thinking about Double Mutants of Enzymes Mildvan, Albert S. Biochemistry, Vol. 43, Issue 46, p. 14517-14520 https://doi.org/10.1021/bi048052e	journal	November 2004
Stimulation of Lignocellulosic Biomass Hydrolysis by Proteins of Glycoside Hydrolase Family 61 Structure and Function of a Large, Enigmatic Family Harris, Paul; Welner, Ditte; McFarland, K. Biochemistry, Vol. 49, Issue 15, p. 3305-3316 https://doi.org/10.1021/bi100009p	journal	April 2010
Comparative Study of Two Chitin-Active and Two Cellulose-Active AA10-Type Lytic Polysaccharide Monooxygenases Forsberg, Zarah; Røhr, Åsmund Kjendseth; Mekasha, Sophanit Biochemistry, Vol. 53, Issue 10 https://doi.org/10.1021/bi5000433	journal	March 2014
The Copper Active Site of CBM33 Polysaccharide Oxygenases Hemsworth, Glyn R.; Taylor, Edward J.; Kim, Robbert Q. Journal of the American Chemical Society, Vol. 135, Issue 16 https://doi.org/10.1021/ja402106e	journal	April 2013
Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning Brumbauer, Anikó; Johansson, Göte; Réczey, Kati Bioseparation, Vol. 7, Issue 6, p. 287-295 https://doi.org/10.1023/A:1008153418019	journal	November 1999
Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp Suurnäkki, A.; Tenkanen, M.; Siika-aho, M. Cellulose, Vol. 7, Issue 2, p. 189-209 https://doi.org/10.1023/A:1009280109519	journal	June 2000
Continuous cultures of fused cells secreting antibody of predefined specificity Köhler, G.; Milstein, C. Nature, Vol. 256, Issue 5517, p. 495-497 https://doi.org/10.1038/256495a0	journal	August 1975
Replacing the complementarity-determining regions in a human antibody with those from a mouse Jones, Peter T.; Dear, Paul H.; Foote, Jefferson Nature, Vol. 321, Issue 6069, p. 522-525 https://doi.org/10.1038/321522a0	journal	May 1986
Reshaping human antibodies for therapy Riechmann, Lutz; Clark, Michael; Waldmann, Herman Nature, Vol. 332, Issue 6162, p. 323-327 https://doi.org/10.1038/332323a0	journal	March 1988
A novel genetic system to detect protein–protein interactions Fields, Stanley; Song, Ok-kyu Nature, Vol. 340, Issue 6230, p. 245-246 https://doi.org/10.1038/340245a0	journal	July 1989
A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I Ståhlberg, Jerry; Johansson, Gunnar; Pettersson, Göran Nature Biotechnology, Vol. 9, Issue 3, p. 286-290 https://doi.org/10.1038/nbt0391-286	journal	March 1991
Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina) Martinez, Diego; Berka, Randy M.; Henrissat, Bernard Nature Biotechnology, Vol. 26, Issue 5 https://doi.org/10.1038/nbt1403	journal	May 2008
Discovery and characterization of a new family of lytic polysaccharide monooxygenases Hemsworth, Glyn R.; Henrissat, Bernard; Davies, Gideon J. Nature Chemical Biology, Vol. 10, Issue 2 https://doi.org/10.1038/nchembio.1417	journal	December 2013
Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase Lo Leggio, Leila; Simmons, Thomas J.; Poulsen, Jens-Christian N. Nature Communications, Vol. 6, Issue 1 https://doi.org/10.1038/ncomms6961	journal	January 2015
Activity, stability and 3-D structure of the Cu( ii ) form of a chitin-active lytic polysaccharide monooxygenase from Bacillus amyloliquefaciens Gregory, Rebecca C.; Hemsworth, Glyn R.; Turkenburg, Johan P. Dalton Transactions, Vol. 45, Issue 42, p. 16904-16912 https://doi.org/10.1039/C6DT02793H	journal	January 2016
Properties of cellulolytic enzyme systems Wood, Thomas M. Biochemical Society Transactions, Vol. 13, Issue 2, p. 407-410 https://doi.org/10.1042/bst0130407	journal	April 1985
Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei Karlsson, Johan; Saloheimo, Markku; Siika-aho, Matti European Journal of Biochemistry, Vol. 268, Issue 24, p. 6498-6507 https://doi.org/10.1046/j.0014-2956.2001.02605.x	journal	December 2001
The relationship between thermal stability and pH optimum studied with wild-type and mutant Trichoderma reesei cellobiohydrolase Cel7A: Stability of cellobiohydrolase Cel7A Boer, Harry; Koivula, Anu European Journal of Biochemistry, Vol. 270, Issue 5 https://doi.org/10.1046/j.1432-1033.2003.03431.x	journal	March 2003
Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components Quinlan, R. J.; Sweeney, M. D.; Lo Leggio, L. Proceedings of the National Academy of Sciences, Vol. 108, Issue 37, p. 15079-15084 https://doi.org/10.1073/pnas.1105776108	journal	August 2011
NMR structure of a lytic polysaccharide monooxygenase provides insight into copper binding, protein dynamics, and substrate interactions Aachmann, F. L.; Sorlie, M.; Skjak-Braek, G. Proceedings of the National Academy of Sciences, Vol. 109, Issue 46 https://doi.org/10.1073/pnas.1208822109	journal	October 2012
Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases Forsberg, Z.; Mackenzie, A. K.; Sorlie, M. Proceedings of the National Academy of Sciences, Vol. 111, Issue 23 https://doi.org/10.1073/pnas.1402771111	journal	May 2014
Integration of bacterial lytic polysaccharide monooxygenases into designer cellulosomes promotes enhanced cellulose degradation Arfi, Y.; Shamshoum, M.; Rogachev, I. Proceedings of the National Academy of Sciences, Vol. 111, Issue 25 https://doi.org/10.1073/pnas.1404148111	journal	June 2014
Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization Chiu, Elaine; Hijnen, Marcel; Bunker, Richard D. Proceedings of the National Academy of Sciences, Vol. 112, Issue 13, p. 3973-3978 https://doi.org/10.1073/pnas.1418798112	journal	March 2015
Transformation of Aspergillus nidulans by using a trpC plasmid. Yelton, M. M.; Hamer, J. E.; Timberlake, W. E. Proceedings of the National Academy of Sciences, Vol. 81, Issue 5, p. 1470-1474 https://doi.org/10.1073/pnas.81.5.1470	journal	March 1984
Rapid and efficient site-specific mutagenesis without phenotypic selection. Kunkel, T. A. Proceedings of the National Academy of Sciences, Vol. 82, Issue 2, p. 488-492 https://doi.org/10.1073/pnas.82.2.488	journal	January 1985
Improved tools for biological sequence comparison. Pearson, W. R.; Lipman, D. J. Proceedings of the National Academy of Sciences, Vol. 85, Issue 8, p. 2444-2448 https://doi.org/10.1073/pnas.85.8.2444	journal	April 1988
Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor. Bajar, A.; Podila, G. K.; Kolattukudy, P. E. Proceedings of the National Academy of Sciences, Vol. 88, Issue 18, p. 8208-8212 https://doi.org/10.1073/pnas.88.18.8208	journal	September 1991
Amino acid substitution matrices from protein blocks. Henikoff, S.; Henikoff, J. G. Proceedings of the National Academy of Sciences, Vol. 89, Issue 22, p. 10915-10919 https://doi.org/10.1073/pnas.89.22.10915	journal	November 1992
Applications and statistics for multiple high-scoring segments in molecular sequences. Karlin, S.; Altschul, S. F. Proceedings of the National Academy of Sciences, Vol. 90, Issue 12, p. 5873-5877 https://doi.org/10.1073/pnas.90.12.5873	journal	June 1993
ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei Aro, Nina; Saloheimo, Anu; Ilmén, Marja Journal of Biological Chemistry, Vol. 276, Issue 26, p. 24309-24314 https://doi.org/10.1074/jbc.M003624200	journal	April 2001
Crystal Structure and Computational Characterization of the Lytic Polysaccharide Monooxygenase GH61D from the Basidiomycota Fungus Phanerochaete chrysosporium Wu, Miao; Beckham, Gregg T.; Larsson, Anna M. Journal of Biological Chemistry, Vol. 288, Issue 18 https://doi.org/10.1074/jbc.M113.459396	journal	March 2013
Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-ray Photoreduction Gudmundsson, Mikael; Kim, Seonah; Wu, Miao Journal of Biological Chemistry, Vol. 289, Issue 27, p. 18782-18792 https://doi.org/10.1074/jbc.M114.563494	journal	May 2014
Temperature Effects on Kinetic Parameters and Substrate Affinity of Cel7A Cellobiohydrolases Sørensen, Trine Holst; Cruys-Bagger, Nicolaj; Windahl, Michael Skovbo Journal of Biological Chemistry, Vol. 290, Issue 36, p. 22193-22202 https://doi.org/10.1074/jbc.M115.658930	journal	July 2015
Structural and Functional Characterization of a Lytic Polysaccharide Monooxygenase with Broad Substrate Specificity Borisova, Anna S.; Isaksen, Trine; Dimarogona, Maria Journal of Biological Chemistry, Vol. 290, Issue 38 https://doi.org/10.1074/jbc.M115.660183	journal	July 2015
Structural and Functional Analysis of a Lytic Polysaccharide Monooxygenase Important for Efficient Utilization of Chitin in Cellvibrio japonicus Forsberg, Zarah; Nelson, Cassandra E.; Dalhus, Bjørn Journal of Biological Chemistry, Vol. 291, Issue 14 https://doi.org/10.1074/jbc.M115.700161	journal	February 2016
The Contribution of Non-catalytic Carbohydrate Binding Modules to the Activity of Lytic Polysaccharide Monooxygenases Crouch, Lucy I.; Labourel, Aurore; Walton, Paul H. Journal of Biological Chemistry, Vol. 291, Issue 14, p. 7439-7449 https://doi.org/10.1074/jbc.M115.702365	journal	January 2016
Heterogeneity in the Histidine-brace Copper Coordination Sphere in Auxiliary Activity Family 10 (AA10) Lytic Polysaccharide Monooxygenases Chaplin, Amanda K.; Wilson, Michael T.; Hough, Michael A. Journal of Biological Chemistry, Vol. 291, Issue 24, p. 12838-12850 https://doi.org/10.1074/jbc.M116.722447	journal	April 2016
Crystal Structure and Binding Properties of the Serratia marcescens Chitin-binding Protein CBP21 Vaaje-Kolstad, G.; Houston, D. R.; Riemen, A. H. K. Journal of Biological Chemistry, Vol. 280, Issue 12 https://doi.org/10.1074/jbc.M407175200	journal	December 2004
The Non-catalytic Chitin-binding Protein CBP21 from Serratia marcescens Is Essential for Chitin Degradation Vaaje-Kolstad, Gustav; Horn, Svein J.; van Aalten, Daan M. F. Journal of Biological Chemistry, Vol. 280, Issue 31 https://doi.org/10.1074/jbc.M504468200	journal	June 2005
Structure of Cellulases and Their Applications Ohmiya, Kunio; Sakka, Kazuo; Karita, Shuichi Biotechnology and Genetic Engineering Reviews, Vol. 14, Issue 1, p. 365-414 https://doi.org/10.1080/02648725.1997.10647949	journal	April 1997
R: A Language for Data Analysis and Graphics Ihaka, Ross; Gentleman, Robert Journal of Computational and Graphical Statistics, Vol. 5, Issue 3 https://doi.org/10.1080/10618600.1996.10474713	journal	September 1996
Oligonucleotide-directed mutagenesis using M13-derived vectors: an efficient and general procedure for the production of point mutations in any fragment of DNA Zoller, Mark J.; Smith, Michael Nucleic Acids Research, Vol. 10, Issue 20, p. 6487-6500 https://doi.org/10.1093/nar/10.20.6487	journal	January 1982
Improved oligonudeotide site-directed rautagenesis using M13 vectors Carter, Paul; Bedouelle, Hugues; Winter, Greg Nucleic Acids Research, Vol. 13, Issue 12, p. 4431-4443 https://doi.org/10.1093/nar/13.12.4431	journal	January 1985
Construction of mutant and chimeric genes using the polymerase chain reaction Vallette, Francois; Mege, Emmanuelle; Reiss, Allison Nucleic Acids Research, Vol. 17, Issue 2, p. 723-733 https://doi.org/10.1093/nar/17.2.723	journal	January 1989
Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase) Ooi, Toshihiko; Shinmyo, Atsuhiko; Okada, Hirosuke Nucleic Acids Research, Vol. 18, Issue 19 https://doi.org/10.1093/nar/18.19.5884	journal	January 1990
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J. Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680 https://doi.org/10.1093/nar/22.22.4673	journal	January 1994
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs Altschul, Stephen F.; Madden, Thomas L.; Schäffer, Alejandro A. Nucleic Acids Research, Vol. 25, Issue 17, p. 3389-3402 https://doi.org/10.1093/nar/25.17.3389	journal	September 1997
Importance of Hydrogen-Bond Formation in Stabilizing the Transition State of Subtilisin Wells, J. A.; Cunningham, B. C.; Graycar, T. P. Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 317, Issue 1540, p. 415-423 https://doi.org/10.1098/rsta.1986.0051	journal	April 1986
The Streptomyces reticuli α-chitin-binding protein CHB2 and its gene Kolbe, S.; Fischer, S.; Becirevic, A. Microbiology, Vol. 144, Issue 5, p. 1291-1297 https://doi.org/10.1099/00221287-144-5-1291	journal	May 1998
Lytic polysaccharide monooxygenases: a crystallographer's view on a new class of biomass-degrading enzymes Frandsen, Kristian E. H.; Lo Leggio, Leila IUCrJ, Vol. 3, Issue 6 https://doi.org/10.1107/S2052252516014147	journal	October 2016
Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis TOMME, Peter; TILBEURGH, Herman; PETTERSSON, Goran European Journal of Biochemistry, Vol. 170, Issue 3, p. 575-581 https://doi.org/10.1111/j.1432-1033.1988.tb13736.x	journal	January 1988
Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli Spilliaert, Remi; Hreggvidsson, Gudmundur Oli; Kristjansson, Jakob Kristjan European Journal of Biochemistry, Vol. 224, Issue 3, p. 923-930 https://doi.org/10.1111/j.1432-1033.1994.00923.x	journal	September 1994
cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast Saloheimo, Markku; Nakari-SetaLa, Tiina; Tenkanen, Maija European Journal of Biochemistry, Vol. 249, Issue 2, p. 584-591 https://doi.org/10.1111/j.1432-1033.1997.00584.x	journal	October 1997
Isolation and partial characterization of cellulose-degrading strain of Streptomyces sp. LX from soil Li, X.; Gao, P. Letters in Applied Microbiology, Vol. 22, Issue 3, p. 209-213 https://doi.org/10.1111/j.1472-765X.1996.tb01145.x	journal	March 1996
Genetic transformation of intact cells of Bacillus subtilis by electroporation Brigidi, P.; Rossi, E.; Bertarini, M.L. FEMS Microbiology Letters, Vol. 67, Issue 1-2, p. 135-138 https://doi.org/10.1111/j.1574-6968.1990.tb13850.x	journal	January 1990
Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei Te'o, Valentino S.J.; Cziferszky, Angela E.; Bergquist, Peter L. FEMS Microbiology Letters, Vol. 190, Issue 1, p. 13-19 https://doi.org/10.1111/j.1574-6968.2000.tb09255.x	journal	September 2000
Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs Jakobovits, Aya; Green, Larry L.; Hardy, Margaret C. Annals of the New York Academy of Sciences, Vol. 764, Issue 1, p. 525-535 https://doi.org/10.1111/j.1749-6632.1995.tb55878.x	journal	September 1995
Reshaping human antibodies: grafting an antilysozyme activity Verhoeyen, M.; Milstein, C.; Winter, G. Science, Vol. 239, Issue 4847, p. 1534-1536 https://doi.org/10.1126/science.2451287	journal	March 1988
Engineered Pentafunctional Minicellulosome for Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae Liang, Youyun; Si, Tong; Ang, Ee Lui Applied and Environmental Microbiology, Vol. 80, Issue 21, p. 6677-6684 https://doi.org/10.1128/AEM.02070-14	journal	August 2014
Increased Crystalline Cellulose Activity via Combinations of Amino Acid Changes in the Family 9 Catalytic Domain and Family 3c Cellulose Binding Module of Thermobifida fusca Cel9A Li, Y.; Irwin, D. C.; Wilson, D. B. Applied and Environmental Microbiology, Vol. 76, Issue 8 https://doi.org/10.1128/AEM.02735-09	journal	February 2010
Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae. Li, X. L.; Ljungdahl, L. G. Applied and environmental microbiology, Vol. 62, Issue 1 https://doi.org/10.1128/AEM.62.1.209-213.1996	journal	January 1996
Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Ilmén, M.; Saloheimo, A.; Onnela, M. L. Applied and environmental microbiology, Vol. 63, Issue 4 https://doi.org/10.1128/AEM.63.4.1298-1306.1997	journal	January 1997
Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides. Hu, Q. J.; Bautista, C.; Edwards, G. M. Molecular and Cellular Biology, Vol. 11, Issue 11, p. 5792-5799 https://doi.org/10.1128/MCB.11.11.5792	journal	November 1991
Efficient transformation of Dictyostelium discoideum amoebae. Barclay, S. L.; Meller, E. Molecular and Cellular Biology, Vol. 3, Issue 12, p. 2117-2130 https://doi.org/10.1128/MCB.3.12.2117	journal	December 1983
Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Hynes, M. J.; Corrick, C. M.; King, J. A. Molecular and Cellular Biology, Vol. 3, Issue 8, p. 1430-1439 https://doi.org/10.1128/MCB.3.8.1430	journal	August 1983
Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori. Nunberg, J. H.; Meade, J. H.; Cole, G. Molecular and Cellular Biology, Vol. 4, Issue 11, p. 2306-2315 https://doi.org/10.1128/MCB.4.11.2306	journal	November 1984
Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea Filho, Edivaldo Ximenes Ferreira Canadian Journal of Microbiology, Vol. 42, Issue 1, p. 1-5 https://doi.org/10.1139/m96-001	journal	January 1996
Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates Häkkinen, Mari; Arvas, Mikko; Oja, Merja Microbial Cell Factories, Vol. 11, Issue 1 https://doi.org/10.1186/1475-2859-11-134	journal	January 2012
Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes Levasseur, Anthony; Drula, Elodie; Lombard, Vincent Biotechnology for Biofuels, Vol. 6, Issue 1, Article No. 41 https://doi.org/10.1186/1754-6834-6-41	journal	January 2013
A quantitative indicator diagram for lytic polysaccharide monooxygenases reveals the role of aromatic surface residues in HjLPMO9A regioselectivity Danneels, Barbara; Tanghe, Magali; Joosten, Henk-Jan PLOS ONE, Vol. 12, Issue 5 https://doi.org/10.1371/journal.pone.0178446	journal	May 2017
The Vibrio cholerae Colonization Factor GbpA Possesses a Modular Structure that Governs Binding to Different Host Surfaces Wong, Edmond; Vaaje-Kolstad, Gustav; Ghosh, Avishek PLoS Pathogens, Vol. 8, Issue 1 https://doi.org/10.1371/journal.ppat.1002373	journal	January 2012
Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor: Investigation of Yields, Kinetics, and Enzymatic Digestibilities of Solids Schell, Daniel J.; Farmer, Jody; Newman, Millie Applied Biochemistry and Biotechnology, Vol. 105, Issue 1-3, p. 69-86 https://doi.org/10.1385/ABAB:105:1-3:69	journal	January 2003
R: A Language for Data Analysis and Graphics Ihaka, Ross; Gentleman, Robert Journal of Computational and Graphical Statistics, Vol. 5, Issue 3 https://doi.org/10.2307/1390807	journal	September 1996
Identification of a Genomic Region Containing a Novel Promoter Resistant to Glucose Repression and Over-Expression of β-Glucosidase Gene in Hypocrea orientalis EU7-22 Long, Chuannan; Cheng, Yijin; Gan, Lihui International Journal of Molecular Sciences, Vol. 14, Issue 4, p. 8479-8490 https://doi.org/10.3390/ijms14048479	journal	April 2013
A Structural Overview of gh61 Proteins – Fungal Cellulose Degrading Polysaccharide Monooxygenases Leggio, Leila Lo; Welner, Ditte; De Maria, Leonardo Computational and Structural Biotechnology Journal, Vol. 2, Issue 3 https://doi.org/10.5936/csbj.201209019	journal	September 2012

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