Genome-centric view of carbon processing in thawing permafrost

Woodcroft, Ben J.; Singleton, Caitlin M.; Boyd, Joel A.; Evans, Paul N.; Emerson, Joanne B.; Zayed, Ahmed A.  F.; Hoelzle, Robert D.; Lamberton, Timothy O.; McCalley, Carmody K.; Hodgkins, Suzanne B.; Wilson, Rachel M.; Purvine, Samuel O.; Nicora, Carrie D.; Li, Changsheng; Frolking, Steve; Chanton, Jeffrey P.; Crill, Patrick M.; Saleska, Scott R.; Rich, Virginia I.; Tyson, Gene W.

doi:10.1038/s41586-018-0338-1

Title: Genome-centric view of carbon processing in thawing permafrost

Journal Article · Mon Jul 16 00:00:00 EDT 2018 · Nature (London)

DOI:https://doi.org/10.1038/s41586-018-0338-1· OSTI ID:1481643

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^[1]; Boyd, Joel A. ^[1]; Evans, Paul N. ^[1];

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^[3];

^[1]; Lamberton, Timothy O. ^[1];

^[4];

^[5]; Wilson, Rachel M. ^[5];

^[6];

^[6]; Li, Changsheng ^[7]; Frolking, Steve ^[7]; Chanton, Jeffrey P. ^[5]; Crill, Patrick M. ^[8];

^[9];

^[3];

^[1]

Univ. of Queensland, Brisbane, QLD (Australia)
The Ohio State Univ., Columbus, OH (United States); Univ. of California, Davis, CA (United States)
The Ohio State Univ., Columbus, OH (United States)
Rochester Institute of Technology, Rochester, NY (United States)
Florida State Univ., Tallahassee, FL (United States)
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Univ. of New Hampshire, Durham, NH (United States)
Stockholm Univ., Stockholm (Sweden)
Univ. of Arizona, Tucson, AZ (United States)

As global temperatures rise, large amounts of carbon sequestered in permafrost are becoming available for microbial degradation. Accurate prediction of carbon gas emissions from thawing permafrost is limited by our understanding of these microbial communities. Here we use metagenomic sequencing of 214 samples from a permafrost thaw gradient to recover 1,529 metagenome-assembled genomes, including many from phyla with poor genomic representation. These genomes reflect the diversity of this complex ecosystem, with genus-level representatives for more than sixty per cent of the community. Meta-omic analysis revealed key populations involved in the degradation of organic matter, including bacteria whose genomes encode a previously undescribed fungal pathway for xylose degradation. Microbial and geochemical data highlight lineages that correlate with the production of greenhouse gases and indicate novel syntrophic relationships. Lastly, our findings link changing biogeochemistry to specific microbial lineages involved in carbon processing, and provide key information for predicting the effects of climate change on permafrost systems.

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Research Organization:: Univ. of Arizona, Tucson, AZ (United States); The Ohio State Univ., Columbus, OH (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Contributing Organization:: Univ. of Queensland, Brisbane, QLD (Australia). Australian Centre for Ecogenomics, School of Chemistry and Molecular Biosciences

Grant/Contract Number:: SC0010580; SC0016440

OSTI ID:: 1481643

Journal Information:: Nature (London), Vol. 560, Issue 7716; Related Information: Raw metagenome and metatranscriptomes from the study: https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA386568; ISSN 0028-0836

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 207 works

Citation information provided by
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Title: Genome-centric view of carbon processing in thawing permafrost

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